[13C9,15N]-phenylalanine | 878339-23-2

• 物质功能分类: 生物化工 - 氨基酸及其衍生物
• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 英文名称: [13C9,15N]-phenylalanine
• 英文别名: [U-(13)C9,(15)N]-α-phenylalanine；L-Phenylalanine-13C9,15N；(2S)-2-(15N)azanyl-3-((1,2,3,4,5,6-13C6)cyclohexatrienyl)(1,2,3-13C3)propanoic acid
• CAS: 878339-23-2
• 化学式: C₉H₁₁NO₂
• 分子量: 175.086
• InChiKey: COLNVLDHVKWLRT-CMLFETTRSA-N

物化性质

• 熔点：
  270-275°C (dec.) (lit.)
• 溶解度：
  超声处理可轻微溶于甲醇和水

计算性质

• 辛醇/水分配系数(LogP)：
  -1.5
• 重原子数：
  12
• 可旋转键数：
  3
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.22
• 拓扑面积：
  63.3
• 氢给体数：
  2
• 氢受体数：
  3

反应信息

• 作为反应物：
  描述：

  [13C9,15N]-phenylalanine 以 乙腈 为溶剂， 反应 18.17h， 生成
  参考文献：
  名称：

  衍生，消旋和分析-对映选择性代谢组学的手性氨基酸标准品制备的简便方法。

  摘要：

  开发了一种简单，可控制和可重现的立体异构化方法（消旋和差向异构化），用于从立体异构纯标准品制备规模化α-氨基酸混合物。只需使用消旋标签衍生化您的氨基酸即可，该消旋标签在目标氨基酸的N-末端掺有尿素键，并在高达95°C的高温下孵育一段确定的时间，直至获得目标d-氨基酸水平。研究的外消旋标记是6-氨基喹啉基-N-羟基琥珀酰亚胺基氨基甲酸酯（AQC），氨基苯基-N-羟基琥珀酰亚胺基氨基甲酸酯（AC）和3-氨基吡啶基-N氨基甲酸-羟基琥珀酰亚胺基酯（APC）。采用这种方法，可以在数分钟内或在几分钟内，制备出可立即使用的，量身定制的，手性均匀的13 C和15 N标记的[U - 13 C 15 N]-氨基酸标准品，并具有所需的d-氨基酸百分比。小时不进行样品清理。在95°C下消旋30分钟的消旋时间将导致d-氨基酸水平为1-5％，而在95°C下6 h消旋时间将提供15-30％的d-氨基酸。外消旋化是由于

  DOI：

  10.1021/acs.analchem.9b00666

文献信息

• Stereochemistry and Mechanism of a Microbial Phenylalanine Aminomutase
  作者：Nishanka Dilini Ratnayake、Udayanga Wanninayake、James H. Geiger、Kevin D. Walker

  DOI：10.1021/ja2030728

  日期：2011.6.8

  The stereochemistry of a phenylalanine aminomutase (PAM) on the andrimid biosynthetic pathway in Pantoea agglomerans (Pa) is reported. PaPAM is a member of the 4-methylidene-1H-imidazol-5(4H)-one (MIO)-dependent family of catalysts and isomerizes (2S)-alpha-phenylalanine to (3S)-beta-phenylalanine, which is the enantiomer of the product made by the mechanistically similar aminomutase TcPAM from Taxus plants. The NH2 and pro-(3S) hydrogen groups at C-alpha and C-beta, respectively, of the substrate are removed and interchanged completely intramolecularly with inversion of configuration at the migration centers to form P-phenylalanine. This is a contrast to the retention of configuration mechanism followed by TcPAM.
• METHOD FOR DIAGNOSING DISEASES
  申请人：Becton, Dickinson and Company

  公开号：EP2208071B1

  公开（公告）日：2012-08-01
• System and method for diagnosing diseases
  申请人：Gelfand Craig A.

  公开号：US20090208923A1

  公开（公告）日：2009-08-20

  One aspect of the invention provides a method of diagnosing a disease condition, comprising measuring presence or amount of a targeted protein or a degradation product of said protein in a collected biological sample as a marker for the disease condition. The targeted protein or degradation product is selected for measurement based on a prior identification of a measurable half-life at a predetermined time period, including the time at which said method is conducted, and correlating said measuring with the presence or absence of the disease condition. The targeted protein or degradation product may be identified by selecting a protein known or suspected to be a diagnostic marker for the disease condition, analyzing degradation of the protein in the collected biological sample, and selecting a protein or degradation product that exhibits a measurable half-life at a predetermined period of time. The analyzing may include identifying degradation product(s) of the protein as a function of time, and half-life of the protein and the degradation product(s).
• US8735085B2
  申请人：——

  公开号：US8735085B2

  公开（公告）日：2014-05-27
• US9673030B2
  申请人：——

  公开号：US9673030B2

  公开（公告）日：2017-06-06