α-D-Galp(1->6)-α-D-Galp(1->6)-D-Galp | 1062-56-2

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: α-D-Galp(1->6)-α-D-Galp(1->6)-D-Galp
• 英文别名: (3R,4S,5R,6R)-6-[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-[[(2S,3R,4S,5R,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxymethyl]oxan-2-yl]oxymethyl]oxane-2,3,4,5-tetrol
• CAS: 1062-56-2
• 化学式: C₁₈H₃₂O₁₆
• 分子量: 504.442
• InChiKey: FBJQEBRMDXPWNX-MEGYGSNGSA-N

物化性质

• 沸点：
  856.6±65.0 °C(Predicted)
• 密度：
  1.80±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -6.9
• 重原子数：
  34
• 可旋转键数：
  7
• 环数：
  3.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  269
• 氢给体数：
  11
• 氢受体数：
  16

反应信息

• 作为产物：
  描述：

  口服葡萄糖 在 Aspergillus niger APC-9319 α-galactosidase 作用下， 以 acetate buffer 为溶剂， 反应 40.0h， 生成 swietenose 、 3-O-α-D-galactopyranosyl-D-galactose 、 α-D-Galp(1->6)-α-D-Galp(1->6)-D-Galp 、 2-O-α-D-galactopyranosyl-D-galactopyranose
  参考文献：
  名称：

  Reverse Reaction ofAspergillus nigerAPC-9319 α-Galactosidase in a Supersaturated Substrate Solution: Production of α-Linked Galactooligosaccharide (α-GOS)

  摘要：

  从含有α-半乳糖苷酶活性的食品加工工业酶制剂中筛选出能有效催化半乳糖逆反应的Aspergillus niger APC-9319 α-半乳糖苷酶。使用过饱和溶液（90%半乳糖[w/v]）进行A. niger APC-9319 α-半乳糖苷酶的逆反应。即使在高的底物浓度下，A. niger APC-9319 α-半乳糖苷酶也没有被抑制，并且能有效催化逆反应。反应产物α-半乳糖寡糖（α-GOS）的产率随着半乳糖初始浓度的增加而大幅增加，达到90%（w/v）时超过50%。此外，酶活性的半衰期约为使用60%半乳糖（w/v）时的三倍。通过A. niger APC-9319 α-半乳糖苷酶的逆反应，从3.0 g半乳糖制备了1.4 g的α-GOS。α-GOS含有58%的α-半乳二糖（α-Gal2），28%的α-半乳三糖，以及14%的大于α-半乳三糖的寡糖。α-Gal2中的主要位置异构体是α-1,6Gal2。

  DOI：

  10.1271/bbb.69.1381

文献信息

• Reverse Reaction of<i>Aspergillus niger</i>APC-9319 α-Galactosidase in a Supersaturated Substrate Solution: Production of α-Linked Galactooligosaccharide (α-GOS)
  作者：Akiko YAMASHITA、Hiroyuki HASHIMOTO、Koki FUJITA、Masamichi OKADA、Shigeharu MORI、Sumio KITAHATA

  DOI：10.1271/bbb.69.1381

  日期：2005.1

  The α-galactosidase that effectively catalyzes a reverse reaction of galactose, Aspergillus niger APC-9319 α-galactosidase, was screened from industrial enzyme preparations for food processing containing α-galactosidase activity. Reverse reaction of A. niger APC-9319 α-galactosidase was performed using a supersaturated solution (90% galactose [w/v]). A. niger APC-9319 α-galactosidase was not inhibited even in high substrate concentration, and effectively catalyzed the reverse reaction. The yield of the reaction product, α-linked galactooligosaccharide (α-GOS), increased greatly as the initial concentration of galactose increased to 90% (w/v), and was more than 50%. Furthermore, the half life of enzyme activity was about three times as long as that using 60% galactose (w/v). α-GOS (1.4 g) was prepared from galactose (3.0 g) by reverse reaction of A. niger APC-9319 α-galactosidase. The α-GOS contained 58% α-galactobiose (α-Gal2), 28% α-galactotriose, and 14% oligosaccharides larger than α-galactotriose. The main component of positional isomers in α-Gal2 was α-1,6Gal2.

  从含有α-半乳糖苷酶活性的食品加工工业酶制剂中筛选出能有效催化半乳糖逆反应的Aspergillus niger APC-9319 α-半乳糖苷酶。使用过饱和溶液（90%半乳糖[w/v]）进行A. niger APC-9319 α-半乳糖苷酶的逆反应。即使在高的底物浓度下，A. niger APC-9319 α-半乳糖苷酶也没有被抑制，并且能有效催化逆反应。反应产物α-半乳糖寡糖（α-GOS）的产率随着半乳糖初始浓度的增加而大幅增加，达到90%（w/v）时超过50%。此外，酶活性的半衰期约为使用60%半乳糖（w/v）时的三倍。通过A. niger APC-9319 α-半乳糖苷酶的逆反应，从3.0 g半乳糖制备了1.4 g的α-GOS。α-GOS含有58%的α-半乳二糖（α-Gal2），28%的α-半乳三糖，以及14%的大于α-半乳三糖的寡糖。α-Gal2中的主要位置异构体是α-1,6Gal2。
• Preparation of α-galactooligoglycosides by cell walls from Cryptococcus laurentii using a novel α-galactosyl donor
  作者：Vladimír Mastihuba、Mária Mastihubová、Miroslav Belák、Jana Dudíková、Elena Karnišová Potocká、Ladislav Petruš

  DOI：10.1016/j.tetasy.2017.07.007

  日期：2017.8

  The cell walls of an acapsular strain of the yeast Cryptococcus laurentii catalyze the regioselective formation of alpha-galactooligosaccharides through self-condensation of 4-nitrophenyl alpha-D-galactopyranoside and of a novel activated ot-galactosyl donor 2,2,2-trifluoroethyl of-o-galactopyranoside. The latter substance can be easily prepared by several methods and is highly soluble in water and therefore can be used in higher initial concentrations suppressing secondary product hydrolysis. The preparative reaction catalyzed by cell walls provided 17.4% and 2% of corresponding 2,2,2-trifluoroethyl galactobioside and galactotrioside, respectively, while the reaction with 4-nitrophenyl alpha-D-galactopyranoside provided the corresponding 4-nitrophenyl galactobioside and galactotrioside in 6.6 and 2.5% yields, respectively. The reactions proceeded with strict alpha-(1 -> 6)-regioselectivity. (C) 2017 Elsevier Ltd. All rights reserved.
• The acid-labile, peripheral chains of the mucilage of Opuntia ficus-indica
  作者：Donald McGarvie、Haralambos Parolis

  DOI：10.1016/s0008-6215(00)85595-0

  日期：1981.7