色酚AS-BS磷酸盐 | 10019-03-1

• 物质功能分类: 有机原料 - 羧酸类化合物及衍生物
• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 中文名称: 色酚AS-BS磷酸盐
• 中文别名: 磷酸萘酚AS-BS,萘酚AS-BS磷酸酯；磷酸萘酚AS-BS；萘酚AS-BS磷酸酯
• 英文名称: N-(3-nitrophenyl)-3-(phosphonooxy)naphthalene-2-carboxamide
• 英文别名: naphthol AS-BS-phosphate；2-Phosphonooxy-naphthalin-3-carbonsaeure-<3-nitro-anilid>；[3-[(3-nitrophenyl)carbamoyl]naphthalen-2-yl] dihydrogen phosphate
• CAS: 10019-03-1
• 化学式: C₁₇H₁₃N₂O₇P
• 分子量: 388.273
• InChiKey: JELNBKZALJBEFQ-UHFFFAOYSA-N

物化性质

• 密度：
  1.611±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.2
• 重原子数：
  27
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  142
• 氢给体数：
  3
• 氢受体数：
  7

安全信息

• 危险品标志：
  Xi
• 危险类别码：
  R36/37/38
• 海关编码：
  2924299090
• 安全说明：
  S26,S36

反应信息

• 作为产物：
  描述：

  2-羟基-3-萘甲酸 在 五氯化磷 、 三氯化磷 作用下， 以 1,4-二氧六环 为溶剂， 反应 3.17h， 生成 色酚AS-BS磷酸盐
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022

文献信息

• REAGENT SYSTEM AND METHOD
  申请人：Conn Costa

  公开号：US20120208287A1

  公开（公告）日：2012-08-16

  The present invention relates generally to a chromogenic compositions and reagents systems and methods therefor.
• IMMUNOASSAY REAGENTS AND METHODS OF USE THEREOF
  申请人：BIOCARE MEDICAL, LLC.

  公开号：US20140057803A1

  公开（公告）日：2014-02-27

  The present invention provide reagents and methods of using the reagents, for example, on automated staining devices, that facilitate detection of two or more antigens in a sample simply and efficiently.
• [EN] REAGENT SYSTEM AND METHOD<br/>[FR] SYSTÈME DE RÉACTIF ET PROCÉDÉ
  申请人：LEICA BIOSYSTEMS MELBOURNE PTY

  公开号：WO2011011826A1

  公开（公告）日：2011-02-03

  The present invention relates generally to a chromogenic compositions and reagents systems and methods therefor.
• Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids
  作者：Satoshi. Fujita、Masayoshi. Momiyama、Yasumitsu. Kondo、Naoto. Kagiyama、Samuel H. Hori、Takeshi. Toru

  DOI：10.1021/ac00080a022

  日期：1994.4.15

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.