色酚AS-D磷酸盐 | 27580-13-8

• 物质功能分类: 有机原料 - 羧酸类化合物及衍生物
• 分子结构分类: 有机化合物 - 苯类化合物 - 萘
• 中文名称: 色酚AS-D磷酸盐
• 英文名称: 3-(phosphonooxy)-N-(o-tolyl)naphthalene-2-carboxamide
• 英文别名: naphthol AS-D-phosphate；2-Phosphonooxy-naphthalin-3-carbonsaeure-o-toluidid；[3-[(2-methylphenyl)carbamoyl]naphthalen-2-yl] dihydrogen phosphate
• CAS: 27580-13-8
• 化学式: C₁₈H₁₆NO₅P
• 分子量: 357.303
• InChiKey: NQMNDWPDYBPJNO-UHFFFAOYSA-N

物化性质

• 密度：
  1.456±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  2.7
• 重原子数：
  25
• 可旋转键数：
  4
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.055
• 拓扑面积：
  95.9
• 氢给体数：
  3
• 氢受体数：
  5

安全信息

• 海关编码：
  2924299090

反应信息

• 作为产物：
  描述：

  2-羟基-3-萘甲酸 在 五氯化磷 、 三氯化磷 作用下， 以 1,4-二氧六环 为溶剂， 反应 3.17h， 生成 色酚AS-D磷酸盐
  参考文献：
  名称：

  Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids

  摘要：

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.

  DOI：

  10.1021/ac00080a022

文献信息

• Method of detecting phosphatase
  申请人：AISIN SEIKI KABUSHIKI KAISHA

  公开号：EP0599270A2

  公开（公告）日：1994-06-01

  An easy operable method of detecting phosphatase which comprises a step for the production of a dye to obtain an azo dye by the reaction of phosphatase in a tissue or cell or on chromosome with a 2'-naphthol AS phosphate, e.g. 3-hydroxy- N-2-biphenyl-2-naphthalenecarboxamide phosphate ester, followed by the reaction between the 2'-naphthol AS phosphate with a diazonium salt; an excitation step for the irradiation of excited light to the azo dye; and a detection step for the detection of fluorescence which is emitted upon irradiation of the excited light. The fluorescence is intense and lasts for a long time.

  一种易于操作的检测磷酸酶的方法，它包括一个生产染料的步骤，通过组织或细胞或 染色体上的磷酸酶与 2'-萘酚 AS 磷酸酯（如 3-羟基-N-2-联苯-2-萘甲酰胺磷酸酯）反 应，得到偶氮染料；一个激发步骤，用于激发组织或细胞或染色体上的磷酸酶。3-hydroxy- N-2-biphenyl-2-naphthalenecarboxamide phosphate ester，然后 2'-naphthol AS phosphate 与重氮盐反应；激发步骤，将激发光照射到偶氮染料上；检测步骤，检测激发光照射后发出的荧光。荧光强度高，持续时间长。
• US5480791A
  申请人：——

  公开号：US5480791A

  公开（公告）日：1996-01-02
• Fluorescent Substrates for Potential Use in Enzyme-Linked Immunosorbent Assay of Membrane-Bound Nucleic Acids
  作者：Satoshi. Fujita、Masayoshi. Momiyama、Yasumitsu. Kondo、Naoto. Kagiyama、Samuel H. Hori、Takeshi. Toru

  DOI：10.1021/ac00080a022

  日期：1994.4.15

  A number of phosphorylated fluorochromes were synthesized and tested for the alkaline phosphatase-linked fluorescence assay of membrane-bound nucleic acids. 3-Hydroxy-N-2'-biphenyl-2-naphthalenecarboxamide phosphate ester (HBNP) was found to be the most suitable for the assay. Nonfluorescent HBNP is hydrolyzed by phospholipase bound to the probe DNA to the highly fluorescent 3-hydroxy-N,2'-biphenyl-2-naphthalenecarboxamide (HBN). HBN is insoluble enough in aqueous medium to precipitate on the nylon membrane. Spots containing as little as 5 fg of lambda DNA can be successfully detected on the membrane with HBNP.