Regioselective Glycosylation of Unprotected Phenyl 1-Thioglycopyranosides with Phenylboronic Acid as a Transient Masking Group
作者:Thomas Hauch Fenger、Robert Madsen
DOI:10.1002/ejoc.201300723
日期:2013.9
A useful protocol is described for the regioselectiveglycosylation of the secondary alcohols in unprotected glycosyl acceptors. Phenyl1-thioglycopyranosides derived from D-glucose, D-galactose, D-glucosamine, L-rhamnose, and L-fucose were treated with phenylboronicacid to install a temporary boronic ester, and then submitted to a Koenigs–Knorr glycosylation with perbenzoylated glucopyranosyl or galactopyranosyl
An exo-β-(1→3)-d-galactanase from Streptomyces sp. provides insights into type II arabinogalactan structure
作者:Naomi X.-Y. Ling、Joanne Lee、Miriam Ellis、Ming-Long Liao、Shaio-Lim Mau、David Guest、Peter H. Janssen、Pavol Kováč、Antony Bacic、Filomena A. Pettolino
DOI:10.1016/j.carres.2012.02.033
日期:2012.5
An exo-beta-(1 -> 3)-D-galactanase (SGalase1) that specifically cleaves the beta-(1 -> 3)-D-galactan backbone of arabinogalactan-proteins (AGPs) was isolated from culture filtrates of a soil Streptomyces sp. Internal peptide sequence information was used to clone and recombinantly express the gene in E. coli. The molecular mass of the isolated enzyme was similar to 45 kDa, similar to the 48.2 kDa mass predicted from the amino acid sequence. The pI, pH and temperature optima for the enzyme were similar to 7.45, 3.8 and 48 degrees C, respectively. The native and recombinant enzymes specifically hydrolysed beta-(1 -> 3)-D-galacto-oligo-or poly-saccharides from the upstream (non-reducing) end, typical of an exo-acting enzyme. A second homologous Streptomyces gene (SGalase2) was also cloned and expressed. SGalase2 was similar in size (47.9 kDa) and enzyme activity to SGalase1 but differed in its pH optimum (pH 5). Both SGalase1 and SGalase2 are predicted to belong to the CAZy glycosyl hydrolase family GH 43 based on activity, sequence homology and phylogenetic analysis. The K-m and V-max of the native exo-beta-(1 -> 3)-D-galactanase for de-arabinosylated gum arabic (dGA) were 19 mg/ml and 9.7 mu mol D-Gal/ min/mg protein, respectively. The activity of these enzymes is well suited for the study of type II galactan structures and provides an important tool for the investigation of the biological role of AGPs in plants. De-arabinosylated gum arabic (dGA) was used as a model to investigate the use of these enzymes in defining type II galactan structure. Exhaustive hydrolysis of dGA resulted in a limited number of oligosaccharide products with a trisaccharide of Gal(2)GlcA(1) predominating. (C) 2012 Elsevier Ltd. All rights reserved.
General synthesis of (1 .fwdarw. 3)-.beta.-D-galacto oligosaccharides and their methyl .beta.-glycosides by a stepwise or a blockwise approach
作者:Pavol Kovac、Richard B. Taylor、Cornelis P. J. Glaudemans