C5H10COOH and C9H18COOH were synthesized carrying a methylated C7-OH. Another racemic C4'-OH hapten (CH2COOH) was prepared starting from naringenin. The haptens elicited variable antibody titers dependent on linker lengths, with short linkers giving the best results. Three antisera were characterized in detail: anti-C7-carboxy-propyloxy-2S-(-)-8-PN (anti-H-11), anti-C7-carboxy-propyloxy-2R-(+)-8-PN (anti-H-10)
合成了7个8-
戊烯基
柚皮素(8-
PN)的
羧酸半抗原,与阳离子化的
牛血清白蛋白偶联,并用于提高兔的抗血清特异性。将两个不同长度的接头(C3H6COOH和C6H12COOH)偶联至C7-OH基团,并分离成各自的对映体,产生前四个半抗原。合成具有C4'-OH偶联的接头C5H10COOH和C9H18COOH的外消旋衍
生物,其带有甲基化的C7-OH。从
柚皮素开始制备另一种外消旋的C4'-OH半抗原(CH2COOH)。半抗原引起的
抗体效价取决于接头的长度,短接头给出的结果最佳。详细描述了三种抗血清:抗C7-羧基丙氧基-2S-(-)-8-
PN(抗H-11),抗C7-羧基丙氧基-2R-(+)-8-
PN(抗H-10)和抗C4' -羧基-甲氧基-rac-8-
PN(抗-H-25)。抗H-10和抗H-11显示约9%的对映异构体交叉反应性,而抗H-11不能区分异
黄腐酚(IX)和8-
PN(84%交叉反应性)。对于抗H