Characterisation of the oligosaccharides produced on hydrolysis of galactomannan with β-d-mannase
作者:Barry V. McCleary、Elizabeth Nurthen、François R. Taravel、Jean-Paul Joseleau
DOI:10.1016/0008-6215(83)88038-0
日期:1983.7
Treatment of hot-water-soluble carob galactomannan with β- d -mannanases from A. niger or lucerne seed affords an array of d -galactose-containing β- d -mannosaccharides as well as β- d -manno-biose, -triose, and -tetraose (lucerne-seed enzyme only). The d -galactose-containing β- d -mannosaccharides of d.p. 3–9 produced by A. niger β- d -mannanase have been characterised, using enzymic, n.m.r., and chemical
摘要用黑曲霉或卢塞恩种子中的β-d-甘露聚糖酶处理热水可溶性角豆半乳甘露聚糖,可得到一系列含d-半乳糖的β-d-甘露糖糖以及β-d-甘露糖二糖,三糖,和-四糖(仅限于琉森种子酶)。黑曲霉β-d-甘露聚糖酶产生的dp 3-9的含d-半乳糖的β-d-甘露糖已通过酶,核磁共振和化学技术表征为6 1-α-d-半乳糖基-β -d-甘露二糖,6 1-α-d-半乳糖基-β-d-甘露三糖,6 3,6 4-di-α-d-半乳糖基-β-d-甘露糖半乳糖(唯一的七糖)和6 3,6 4-di-α-d-半乳糖基-β-d-甘露糖己糖,6 4,6 5-di-α-d-半乳糖基-β-d-甘露糖己糖和6 1,6 3,6 4-tri-α- d-半乳糖基-β-d-甘露半乳糖(唯一的八糖)。还鉴定了四种九糖。没有五糖和六糖。卢塞恩种子β-d-甘露聚糖酶产生相同的支链三糖,四糖和七糖,以及五糖和六糖,其特征为6 1-α-d-半乳糖基-β-d-甘露糖,6