作者:Nicolas Bensel、Martine T. Reymond、Jean-Louis Reymond
DOI:10.1002/1521-3765(20011105)7:21<4604::aid-chem4604>3.0.co;2-z
日期:2001.11.5
libraries generated against the tert-butyl phosphonate hapten 2 and the chloromethyl phosphonate hapten 3 with pivaloyloxymethyl-umbelliferone 1 as a fluorogenic substrate led to the isolation of eleven catalytic antibodies with rate accelerations around kcat/ kuncat = 10(3). The antibodies are not inhibited by the product and accept different acyloxymethyl derivatives of acidic phenols as substrates. The
以新戊酰氧基甲基伞形酮1为荧光底物筛选针对叔丁基膦酸半抗原2和氯甲基膦酸半抗原3产生的单克隆抗体文库,导致分离出11种催化抗体,速率加速约kcat / kuncat = 10(3) 。抗体不受产物抑制,并接受酸性酚的不同酰氧基甲基衍生物作为底物。对于体积大,化学反应性较低的新戊酰氧基甲基,发现活性最高:乙酰氧基甲基或乙酰基酯没有活性。这种差异可能反映了免疫系统对结合和催化中疏水相互作用的偏好。新戊酸酯酶催化抗体可用于活化口服可用的新戊酰氧基甲基前药。