3-amino-8-azido-5-ethyl-6-phenylphenanthridinium chloride | 65282-35-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: 3-amino-8-azido-5-ethyl-6-phenylphenanthridinium chloride
• 英文别名: 8-Azido-5-ethyl-6-phenylphenanthridin-3(5H)-imine--hydrogen chloride (1/1)；8-azido-5-ethyl-6-phenylphenanthridin-3-imine;hydrochloride
• CAS: 65282-35-1
• 化学式: C₂₁H₁₈N₅*Cl
• 分子量: 375.86
• InChiKey: FELYPLLXBBAQQW-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  2.5
• 重原子数：
  27
• 可旋转键数：
  3
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.1
• 拓扑面积：
  44.3
• 氢给体数：
  1
• 氢受体数：
  4

反应信息

• 作为产物：
  描述：

  溴化乙啶 在 sodium nitrite 、 sodium azide 作用下， 以 水 为溶剂， 反应 0.05h， 以14%的产率得到3-amino-8-azido-5-ethyl-6-phenylphenanthridinium chloride
  参考文献：
  名称：

  Pedersen, Steen E., Molecular Pharmacology, 1995, vol. 47, # 1, p. 1 - 9

  摘要：

  DOI：

文献信息

• METHOD FOR DETECTION OF MICROORGANISM AND KIT FOR DETECTION OF MICROORGANISM
  申请人：MORINAGA MILK INDUSTRY CO., LTD.

  公开号：EP1918385A1

  公开（公告）日：2008-05-07

  According to the following steps, live cells, injured cells, VNC cells and dead cells of a microorganism in a test sample are detected by flow cytometry: a) the step of treating the test sample with an enzyme having an activity of decomposing cells other than those of the microorganism, colloidal particles of proteins or lipids existing in the test sample, b) the step of treating the test sample with a topoisomerase poison and/or a DNA gyrase poison. c) the step of treating the test sample treated in the steps a) and b) with a nuclear stain agent, and d) the step of detecting the microorganism in the test sample treated with the nuclear stain agent by flow cytometry.

  根据以下步骤，用流式细胞仪检测测试样品中微生物的活细胞、受伤细胞、VNC 细胞和死细胞： a) 用一种酶处理测试样品，该酶具有分解测试样品中微生物细胞、蛋白质胶体颗粒或脂质以外的细胞的活性、 b) 用拓扑异构酶毒物和/或 DNA 回旋酶毒物处理测试样品。 c) 处理经上述步骤处理过的试验样品的步骤 c) 用核染色剂处理在 a) 和 b) 步骤中处理过的检测样本，以及 d) 用流式细胞仪检测经核染色剂处理的测试样本中的微生物。
• NUCLEIC ACID-DEGRADING AGENT AND METHOD FOR DEGRADATION OF NUCLEIC ACID
  申请人：Morinaga Milk Industry Co., Ltd.

  公开号：EP2090169A1

  公开（公告）日：2009-08-19

  The present invention provides an agent for degrading a nucleic acid, which includes ethidium monoazide as an active ingredient, and is useful as an antibacterial agent such as a bactericide or a disinfectant.

  本发明提供了一种降解核酸的制剂，其活性成分包括单氮化乙啶，可用作杀菌剂或消毒剂等抗菌剂。
• Use of phenanthridium derivatives for distinguishing between intact and membrane-compromised cells using molecular nucleic acid-based techniques
  申请人：Montana State University

  公开号：EP2495338A1

  公开（公告）日：2012-09-05

  The present invention provides novel chemicals and methods for selectively excluding DNA of dead cells from a mixture containing live and dead cells from molecular detection.

  本发明提供了从含有活细胞和死细胞的混合物中选择性地排除死细胞 DNA 以进行分子检测的新型化学物质和方法。
• Firth, William J.; Watkins, Charles L.; Graves, David E., Journal of Heterocyclic Chemistry, 1983, vol. 20, p. 759 - 765
  作者：Firth, William J.、Watkins, Charles L.、Graves, David E.、Yielding, Lerena W.

  DOI：——

  日期：——
• FIRTH, W. J. III;WATKINS, CH. L.;GRAVES, D. E.;YIELDING, L. W., J. HETEROCYCL. CHEM., 1983, 20, N 3, 759-765
  作者：FIRTH, W. J. III、WATKINS, CH. L.、GRAVES, D. E.、YIELDING, L. W.

  DOI：——

  日期：——