8-bromo-N2-phenoxyacetyl 2'-deoxy-3',5'-di-O-acetylguanosine | 1035864-73-3

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: 8-bromo-N2-phenoxyacetyl 2'-deoxy-3',5'-di-O-acetylguanosine
• CAS: 1035864-73-3
• 化学式: C₂₂H₂₂BrN₅O₈
• 分子量: 564.349
• InChiKey: NUBSUYZXZBQRCE-ZMSDIMECSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.68
• 重原子数：
  36.0
• 可旋转键数：
  8.0
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.36
• 拓扑面积：
  163.73
• 氢给体数：
  2.0
• 氢受体数：
  11.0

反应信息

• 作为反应物：
  描述：

  8-bromo-N2-phenoxyacetyl 2'-deoxy-3',5'-di-O-acetylguanosine 、 beta-甲基-2-硝基-苯乙醇 在 三苯基膦 、 偶氮二甲酸二乙酯 作用下， 以 四氢呋喃 为溶剂， 反应 12.0h， 以41%的产率得到
  参考文献：
  名称：

  Trapping and Structural Elucidation of a Very Advanced Intermediate in the Lesion-Extrusion Pathway of hOGG1

  摘要：

  Here we present the first structure of a very advanced intermediate in the lesion-extrusion pathway of a DNA glycosylase, human 8-oxoguanine DNA glycosylase (hOGG1), and a substrate DNA containing a mutagenic lesion, 8-oxoguanine (oxoG). The structure was obtained by irradiation and flash-freezing of a disulfide-cross-linked (DXLed) complex of hOgg1 bound to DNA containing a novel photocaged derivative of oxoG. The X-ray structure reveals that upon irradiation, the oxoG lesion has transited from the exosite to the active site pocket, but has not undergone cleavage by the enzyme. Furthermore, all but one of the specificity-determining interactions between the lesion and the enzyme are unformed in the flashed complex (FC), because active site functionality and elements of the DNA backbone are mispositioned. This structure thus provides a first glimpse into the structure of a very late-stage intermediate in the lesion-extrusion pathway-the latest observed to date for any glycosylase-in which the oxoG has undergone insertion into the enzyme active site following photodeprotection, but the enzyme and DNA have not yet completed the slower process of adjusting to the presence of the lesion in the active site.

  DOI：

  10.1021/ja800821t
• 作为产物：
  描述：

  3'-,5'-bis-(O-acetyl)-N²-(phenoxyacetyl)-2'-deoxyguanosine 在 吡啶 、 N-溴代丁二酰亚胺(NBS) 作用下， 以 乙腈 为溶剂， 反应 15.0h， 以2.59 g的产率得到8-bromo-N2-phenoxyacetyl 2'-deoxy-3',5'-di-O-acetylguanosine
  参考文献：
  名称：

  Trapping and Structural Elucidation of a Very Advanced Intermediate in the Lesion-Extrusion Pathway of hOGG1

  摘要：

  Here we present the first structure of a very advanced intermediate in the lesion-extrusion pathway of a DNA glycosylase, human 8-oxoguanine DNA glycosylase (hOGG1), and a substrate DNA containing a mutagenic lesion, 8-oxoguanine (oxoG). The structure was obtained by irradiation and flash-freezing of a disulfide-cross-linked (DXLed) complex of hOgg1 bound to DNA containing a novel photocaged derivative of oxoG. The X-ray structure reveals that upon irradiation, the oxoG lesion has transited from the exosite to the active site pocket, but has not undergone cleavage by the enzyme. Furthermore, all but one of the specificity-determining interactions between the lesion and the enzyme are unformed in the flashed complex (FC), because active site functionality and elements of the DNA backbone are mispositioned. This structure thus provides a first glimpse into the structure of a very late-stage intermediate in the lesion-extrusion pathway-the latest observed to date for any glycosylase-in which the oxoG has undergone insertion into the enzyme active site following photodeprotection, but the enzyme and DNA have not yet completed the slower process of adjusting to the presence of the lesion in the active site.

  DOI：

  10.1021/ja800821t