Identification of a new carbohydrate-binding site of influenza virus
摘要:
It has recently been shown that the influenza virus can specifically bind the residue of a nonsialylated sulfated oligosaccharide Gal(6SO(3)H)beta 1-4GlcNAc beta (6 ' SLacNAc). To identify by photoaffinity labeling the virion component that binds 6 ' SLacNAc, we synthesized a carbohydrate probe containing a I-125 labeled diazocyclopentadien-2-yl carbonyl group as an aglycone. According to the electrophoretic data, the labeled areas corresponded to a large hemagglutinin subunit, a nucleocapsid protein, and neuraminidase (NA). Probing in the presence of an excess of 6 ' SLacNAc beta-OCH2CH2NHAc glycoside resulted in redistribution of the labeling intensity, with the maximum inhibition being observed for NA. The data obtained indicate that NA is a viral 6 ' SLacNAc-binding protein.