4-(2-琥珀酰亚胺基氧羰基乙基)苯基-10-甲基吖啶鎓-9-羧酸酯 | 87198-89-8

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 中文名称: 4-(2-琥珀酰亚胺基氧羰基乙基)苯基-10-甲基吖啶鎓-9-羧酸酯
• 英文名称: 4-(2-Succinimidyloxycarbonylethyl)phenyl-10-methylacridinium-9-carboxylate
• CAS: 87198-89-8
• 化学式: C28H23FN2O9S
• 分子量: 582.6
• InChiKey: IIEBYJYWJZASMK-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  2.99
• 重原子数：
  41
• 可旋转键数：
  8
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.18
• 拓扑面积：
  159
• 氢给体数：
  0
• 氢受体数：
  10

反应信息

• 作为反应物：
  描述：

  4-(2-琥珀酰亚胺基氧羰基乙基)苯基-10-甲基吖啶鎓-9-羧酸酯 生成
  参考文献：
  名称：

  HAGE, DAVID S.;KAO, PAI C., ANAL. CHEM., 63,(1991) N, C. 586-595

  摘要：

  DOI：

文献信息

• METHOD OF JUDGING TYPE OF HUMAN LEUKOCYTE ANTIGEN
  申请人：Chugai Seiyaku Kabushiki Kaisha

  公开号：EP0519070A1

  公开（公告）日：1992-12-23

  A method of judging the type of a human leukocyte antigen (HLA) which is a human histocompatible antigen playing an important role in tissue transplantation and diagnosis of autoimmune diseases and drug sensitivity, said method comprising hybridizing a probe labeled with an acridinium ester through a linker arm under hybridization conditions to the product of amplification of either cDNA prepared from mRNA contained in the specimen or genomic DNA contained in the specimen; decomposing selectively the labeled portion of the unhybridized probe and that of the probe which is hybridized but has mismatching of one or more bases in the crossing; and determining the residual chemiluminescence. This method makes it possible to judge the subtype of the class II gene of HLA, which has been serologically impossible heretofore.

  一种判断人类白细胞抗原（HLA）类型的方法，HLA是一种人类组织相容性抗原，在组织移植、自身免疫性疾病诊断和药物敏感性方面起着重要作用，所述方法包括在杂交条件下将用吖啶酯标记的探针通过连接臂与标本中含有的mRNA制备的cDNA或标本中含有的基因组DNA的扩增产物杂交；选择性地分解未杂交探针的标记部分和已杂交但有一个或多个碱基不匹配的探针的标记部分；并测定残余的化学发光。这种方法可以判断 HLA 第二类基因的亚型，而这在以前的血清学上是不可能实现的。
• Method of measuring concentration of immunoreactant using electrochemiluminescence
  申请人：TDK Corporation

  公开号：EP0522677A1

  公开（公告）日：1993-01-13

  A method of measuring the concentration of an immunoreactant, such as antigen or antibody, in a liquid sample by use of an electrode on which is immobilized a complementary immunoreactant, such as antibody or antigen, capable of specifically reacting with the immunoreactant. An immunoreactant or complementary immunoreactant labeled with a chemiluminescent acridinium compound is bound to an electrode by an antibody-antigen reaction. By use of the electrode with the labeled immunoreactant bound thereon, electrochemical reduction of dissolved oxygen in an electrolytic solution is carried out to generate hydrogen peroxide on the electrode. The hydrogen peroxide triggers chemiluminescence, and the amount of luminescence is measured. According to the method, the amount of luminescence changes correspondingly to minute change in the concentration of the immunoreactant to be measured in the liquid sample and, therefore, it is possible to measure the concentration of the immunoreactant extremely accurately.

  一种测量液体样品中免疫反应物（如抗原或抗体）浓度的方法，使用的电极上固定有能与免疫反应物发生特异性反应的互补免疫反应物（如抗体或抗原）。用化学发光吖啶化合物标记的免疫反应物或互补免疫反应物通过抗体-抗原反应与电极结合。通过使用结合了标记免疫反应物的电极，电解溶液中的溶解氧被电化学还原，在电极上生成过氧化氢。过氧化氢引发化学发光，发光量被测量。根据该方法，发光量随液体样品中待测免疫反应物浓度的微小变化而相应变化，因此可以极其精确地测量免疫反应物的浓度。
• MEASUREMENT SYSTEM USING WHOLE BLOOD
  申请人：IATRON LABORATORIES, INC.

  公开号：EP0722087A1

  公开（公告）日：1996-07-17

  A method for measuring a substance to be examined, characterized in that a sufficient amount of a whole blood sample is brought into contact with an insoluble carrier coated with a first partner specifically bindable to said substance to be examined for a short period of time; a complex of said substance to be examined and said first partner is brought into contact with a second partner which is labeled and capable of specifically binding to said first partner, said complex being carried on said insoluble carrier; a resulting complex of said insoluble carrier-said first partner-said substance to be examined-said labeled second partner, and a portion without said resulting complex are separated from eath other; and a signal originating from said label contained in one of said separated portions is detected is disclosed. According to the present invention, the substance to be examined in the whole blood sample can be quickly measured, using the whole blood as a sample without pretreatment.

  一种测量待检物质的方法，其特征在于：将足量的全血样品与涂有可与所述待检物质特异性结合的第一伴侣的不溶性载体短时间接触；将所述待检物质和所述第一伴侣的复合物与第二伴侣接触，所述第二伴侣被标记并可与所述第一伴侣特异性结合，所述复合物被携带在所述不溶性载体上；将所述不溶性载体-所述第一伴侣-所述待检物质-所述标记的第二伴侣所形成的复合物以及不含所述复合物的部分彼此分离；检测来自所述分离部分之一所含的所述标记的信号。根据本发明，使用全血作为样本，无需预处理，即可快速测量全血样本中的待检物质。
• Micelle protection assay
  申请人：GEN-PROBE INCORPORATED

  公开号：EP0747701A1

  公开（公告）日：1996-12-11

  The present invention provides methods and compositions for selectively detecting analytes in a homogeneous assay, a heterogeneous assay, or a mixture of the two by contacting a labelled probe:analyte complex with one or more amphiphiles. The invention is also useful for increasing the signal to noise ratio when used in conjunction with other assay systems. In preferred embodiments, the analyte and probe are nucleic acids or proteins.

  本发明提供了通过将标记的探针：分析物复合物与一种或多种双亲化合物接触，在均相检测、异相检测或二者混合物中选择性检测分析物的方法和组合物。本发明与其他检测系统结合使用时，还可提高信噪比。在优选的实施方案中，分析物和探针都是核酸或蛋白质。
• Compositions, methods and kits for determining the presence of Trichomonas Vaginalis in a test sample
  申请人：Gen-Probe Incorporated

  公开号：EP2292792A1

  公开（公告）日：2011-03-09

  An oligonucleotide for use in amplifying a target region of nucleic acid derived from Trichomonas vaginalis, the oligonucleotide having a base sequence consisting of a target binding region at least 10 contiguous bases in length and, optionally, a 5' sequence recognized by an RNA polymerase or which enhances initiation or elongation by an RNA polymerase, wherein the base sequence of the target binding region is perfectly complementary to a sequence contained within the base sequence of SEQ ID NO:53, SEQ ID NO:54, SEQ ID NO:55 or SEQ ID NO:56.

  一种用于扩增来自阴道毛滴虫的核酸的靶区的寡核苷酸，该寡核苷酸的碱基序列由长度至少为 10 个连续碱基的靶结合区和可选的 RNA 聚合酶识别的或增强 RNA 聚合酶的启动或延伸的 5'序列组成，其中靶结合区的碱基序列与 SEQ ID NO：53、SEQ ID NO:54、SEQ ID NO:55 或 SEQ ID NO:56 的碱基序列完全互补。