9-(2,6-dibromophenoxycarbonyl)-10-(3-succinimidyloxycarbonylpropyl)acridinium iodide | 846568-80-7

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: 9-(2,6-dibromophenoxycarbonyl)-10-(3-succinimidyloxycarbonylpropyl)acridinium iodide
• 英文别名: (2,6-Dibromophenyl) 10-[4-(2,5-dioxopyrrolidin-1-yl)oxy-4-oxobutyl]acridin-10-ium-9-carboxylate;iodide
• CAS: 846568-80-7
• 化学式: C₂₈H₂₁Br₂N₂O₆*I
• 分子量: 768.197
• InChiKey: ZBWKJNUVXAOKLR-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  2.42
• 重原子数：
  39
• 可旋转键数：
  9
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.18
• 拓扑面积：
  93.9
• 氢给体数：
  0
• 氢受体数：
  7

反应信息

• 作为产物：
  描述：

  2,6-dibromophenyl acridine-9-carboxylate 、 4-iodo(2,5-dioxopyrrolidin-1-yl) butyrate 以 硝基苯 为溶剂， 反应 0.5h， 以3%的产率得到9-(2,6-dibromophenoxycarbonyl)-10-(3-succinimidyloxycarbonylpropyl)acridinium iodide
  参考文献：
  名称：

  Development and application of a novel acridinium ester for use as a chemiluminescent emitter in nucleic acid hybridisation assays using chemiluminescence quenching

  摘要：

  化学发光吖啶鎓酯（AE）具有高强度发光和极低背景的特点，因此可以开发高灵敏度的配体结合检测方法。在这里，我们将利用这些优势，使用淬灭化学发光法进行均相核酸杂交检测。AE 化学发光通常在高碱性 pH 下启动。我们设计了新型 "活性 "AE，可在与维持核酸杂交相适应的条件下（即 pH 值小于 9）启动。研究发现，甲基红是一种能在这种 pH 值下发挥作用的暗色淬灭剂。化学发光淬灭测定系统的实际应用已通过两种基于分子内和分子间发射器/淬灭器对的核酸杂交测定模型得到了验证。

  DOI：

  10.1039/b811947c

文献信息

• CHEMILUMINESCENT PROBES FOR MULTIPLEX MOLECULAR QUANTIFICATION AND USES THEREOF
  申请人：BROWNE Kenneth A.

  公开号：US20120231459A1

  公开（公告）日：2012-09-13

  A novel method is disclosed for simultaneous detection and quantification of two or more nucleic acid targets, without need for amplification. The method depends on spectral-temporal resolution of chemiluminescence emitted from independent hybridization-induced chemiluminescent signal (HICS) probes. The utility of this method has been demonstrated by use of resolvable N-linked acridinium and 2,7-dimethoxyacridinium ester labeled probes in a homogeneous assay for sensitive and simultaneous independent quantification of several bacterial and fungal target sequences. Compositions and kits for practicing the method of the present invention are also disclosed.

  本文揭示了一种新的方法，用于同时检测和定量两个或更多核酸靶标，无需扩增。该方法依赖于独立杂交诱导化学发光信号（HICS）探针发出的化学发光的光谱-时间分辨率。通过在灵敏和同时独立定量多个细菌和真菌靶序列的均相分析中使用可分辨的N-连接丙二酰胺和2,7-二甲氧基丙二酰胺酯标记的探针，证明了该方法的实用性。还公开了用于实施本发明方法的组合物和试剂盒。
• ESTIMATION OF ACTIVITY OR INHIBITION OF PROCESSES INVOLVED IN NUCLEIC ACID MODIFICATION USING CHEMILUMINESCENCE QUENCHING
  申请人：Molecular Light Technology Research Limited

  公开号：EP1658381A2

  公开（公告）日：2006-05-24
• Estimation of activity or inhibition of processes involved in nucleic acid modification using chemiluminescence quenching
  申请人：Weeks Ian

  公开号：US20070077639A1

  公开（公告）日：2007-04-05

  A method for determining the activity of a substance capable of altering the structure of a nucleic acid molecule from a first to a second state which is based upon the use of labelled nucleic acid molecules and/or complementary oligonucleotides. The label is a chemiluminescent molecule and a corresponding quencher molecule whose optical properties are different depending upon whether the nucleic acid molecule exist in said first or second state.
• [EN] ESTIMATION OF ACTIVITY OR INHIBITION OF PROCESSES INVOLVED IN NUCLEIC ACID MODIFICATION USING CHEMILUMINESCENCE QUENCHING<br/>[FR] ESTIMATION DE L'ACTIVITE OU DE L'INHIBITION DE PROCESSUS INTERVENANT DANS LA MODIFICATION DE L'ACIDE NUCLEIQUE AU MOYEN DE L'EXTINCTION DE LA CHIMIOLUMINESCENCE
  申请人：MOLECULAR LIGHT TECH RES LTD

  公开号：WO2005021784A2

  公开（公告）日：2005-03-10

  A method for determining the activity of a substance capable of altering the structure of a nucleic acid molecule from a first to a second state which is based upon the use of labelled nucleic acid molecules and/or complementary oligonucleotides. The label is a chemiluminescent molecule and a corresponding quencher molecule whose optical properties are different depending upon whether the nucleic acid molecule exist in said first or second state.