Peptide and/or protein quantitation methods, kits, and compositions, particularly useful for mass spectrometry, are provided herein based on a bathocuproine-based composition complex such as bathocuproinedisulfonic acid disodium salt hydrate complex. The methods are one-step rapid absorbance methods using small sample volumes. They produce a robust signal with high signal to background ratio and accurately quantitate even complex peptide mixtures with low variability and high sensitivity.
本文提供的肽和/或蛋白质定量方法、试剂盒和组合物,特别适用于质谱分析,其基础是浴己新基组合物复合物,如浴己新二
磺酸二钠盐
水合物复合物。这些方法是使用少量样品的一步快速吸光法。它们能产生稳定的信号,信噪比高,即使是复杂的肽混合物也能准确定量,而且变异性小,灵敏度高。