Process for producing dipeptide,l-amino acid amide hydrolase to be used therein and process for producing l-amino acid amide hydrolase
申请人:Nozaki Hiroyuki
公开号:US20050054067A1
公开(公告)日:2005-03-10
A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus
erwinia,
genus
Rhodococcus,
genus
Chryseobacterium,
genus
Micrococcus,
genus
Cryptococcus,
genus
Trichosporion,
genus
Rhodosporidium,
genus
Sporobolomyces,
genus
Tremela,
genus
Torulaspora,
genus
Sterigmatomyces
or genus
Rhodotorula.
The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
提供一种工业上优势的方法,通过一个便捷的途径,使用成本较低且易得的材料开始,生产二肽。通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物,从培养物中分离的微生物细胞或经过处理的微生物细胞产物来制备二肽。从属于Erwinia属,Rhodococcus属,Chryseobacterium属,Micrococcus属,Cryptococcus属,Trichosporion属,Rhodosporidium属,Sporobolomyces属,Tremela属,Torulaspora属,Sterigmatomyces属或Rhodotorula属的微生物中获得L-氨基酸酰胺水解酶。该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。