8-OHdG

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: 8-OHdG
• 英文别名: 8-Hydroxy-2'-deoxyguanosine；8-hydroxydeoxyguanosine；8-hydroxy-2′-deoxyguanosine；8-oxo-dG；8-Hydroxy-2-desoxyguanosine；2-amino-9-[4-hydroxy-5-(hydroxymethyl)oxolan-2-yl]-1,7-dihydropurine-6,8-dione
• 化学式: C₁₀H₁₃N₅O₅
• 分子量: 283.244
• InChiKey: HCAJQHYUCKICQH-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -2.6
• 重原子数：
  20
• 可旋转键数：
  2
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  150
• 氢给体数：
  5
• 氢受体数：
  5

反应信息

• 作为反应物：
  描述：

  8-OHdG 在 potassium hexacyanoferrate(III) 作用下， 以 phosphate buffer 为溶剂， 反应 24.0h， 生成 spiroiminodihydantoin
  参考文献：
  名称：

  三重激发苯乙酮和α-氧取代衍生物通过Norrish I型裂解产生的自由基对核苷氧化（dG和8-oxodG）的比较光力学研究（自旋捕获、EPR光谱、瞬态动力学、光产物）

  摘要：

  研究了一系列苯乙酮 (AP-X) 和二苯甲酮 (BP) 对 2'-脱氧鸟苷 (dG) 及其衍生物 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) 的光氧化. 苯甲酰基发色团的良好吸收特性使三线态酮的时间分辨光谱能够通过 dG 和 8-oxodG 评估其淬灭动力学。虽然苯乙酮 (AP)、2-乙酰氧基苯乙酮 (AP-OAc) 和二苯甲酮 (BP) 的光解不产生自由基（A 组酮），但羟甲基取代的衍生物 2-羟基苯乙酮 (AP-OH) 和 2-叔-丁氧基苯乙酮 (AP-O(t)Bu) 通过 α 裂解（B 组酮）产生以碳为中心的自由基。对于后一种酮，这被自旋陷阱 5 的 EPR 研究证实，5-二甲基吡咯啉 N-氧化物 (DMPO) 及其三线态寿命比未取代的苯乙酮短。两组酮均光氧化 dG 和 8-oxodG；在 dG 和 AP-OH 的情况下，氧化产物是螺亚氨基二乙内酰脲和胍释放产物

  DOI：

  10.1021/ja017600y

文献信息

• METHODS AND COMPOSITIONS RELATED TO MODIFIED GUANINE BASES FOR CONTROLLING OFF-TARGET EFFECTS IN RNA INTERFERENCE
  申请人：Burrows Cynthia J.

  公开号：US20120095077A1

  公开（公告）日：2012-04-19

  Disclosed are compositions and methods related to modified nucleobases. Also disclosed are compositions and methods related to modified interfering RNAs. Also disclosed are compositions and methods related to modified guanine bases for controlling off-target effects in RNA interference.

  本发明涉及修饰核苷酸的组合物和方法。还涉及修饰干扰RNA的组合物和方法。还涉及修饰鸟嘌呤碱基以控制RNA干扰中的非特异性效应的组合物和方法。
• Polymer
  申请人：CANON KABUSHIKI KAISHA

  公开号：US10577481B2

  公开（公告）日：2020-03-03

  Provided is a polymer that can detect a low-molecular weight compound, in particular, 8-OHdG with high sensitivity and can be repeatedly used. Specifically, provided is a polymer including repeating units each represented by the following formula (1). In the formula (1), R1 and R2 are each independent for each repeating unit, R1 represents a hydrogen atom or an alkyl group, and R2 represents a group represented by the following formula (2) in at least one repeating unit, and represents a phenyl group, a hydroxyl group, an amino group, or a carboxyl group in any other repeating unit, and the group may be substituted with an alkyl group. In the formula (2), R3 represents a hydrogen atom, an alkyl group, or an aryl group, L represents a divalent linking group, and * represents a site to be bonded to the carbon atom bonding to R1 in formula (1).

  本发明提供了一种聚合物，它可以高灵敏度地检测低分子量化合物，特别是 8-OHdG，并且可以重复使用。具体地说，本发明提供了一种聚合物，包括由下式（1）表示的重复单元。在式 (1) 中，R1 和 R2 对于每个重复单元都是独立的，R1 代表氢原子或烷基，R2 在至少一个重复单元中代表下式 (2) 所代表的基团，在任何其他重复单元中代表苯基、羟基、氨基或羧基，该基团可被烷基取代。在式（2）中，R3 代表氢原子、烷基或芳基，L 代表二价连接基团，* 代表与式（1）中与 R1 键合的碳原子键合的位点。
• MAPPING THE LOCATION, TYPE AND STRAND OF DAMAGED AND/OR MISMATCHED NUCLEOTIDES IN DOUBLE-STRANDED DNA
  申请人：New England Biolabs, Inc.

  公开号：EP3710596A1

  公开（公告）日：2020-09-23
• Mapping the Location, Type and Strand of Damaged and/or Mismatched Nucleotides in Double-Stranded DNA
  申请人：New England Biolabs, Inc.

  公开号：US20200325533A1

  公开（公告）日：2020-10-15

  Providing herein, among other things, is a method comprising incubating a double-stranded nucleic acid having a nick with a nick translating activity, a ligase, and a nucleotide mix comprising at least one modified nucleotide, to generate a product comprising a patch of a newly synthesized strand of a duplex nucleic acid containing a plurality of modified nucleoside monophosphates that are at or adjacent to the site of the nick. In some embodiments, the method may be used to map damaged nucleoside monophosphates in a nucleic acid. Compositions and kits for use in performing the method are also provided.
• [EN] METHODS AND COMPOSITIONS RELATED TO MODIFIED GUANINE BASES FOR CONTROLLING OFF-TARGET EFFECTS IN RNA INTERFERENCE<br/>[FR] PROCÉDÉS ET COMPOSITIONS ASSOCIÉS À DES BASES GUANINE MODIFIÉES DESTINÉS À CONTRÔLER LES EFFETS DE FAUX-POSITIFS DANS L'INTERFÉRENCE PAR L'ARN
  申请人：UNIV UTAH RES FOUND

  公开号：WO2010111290A1

  公开（公告）日：2010-09-30

  Disclosed are compositions and methods related to modified nucleobases. Also disclosed are compositions and methods related to modified interfering RNAs. Also disclosed are compositions and methods related to modified guanine bases for controlling off-target effects in RNA interference.