L-精氨酸丁酯 | 142382-85-2

• 分子结构分类: 有机化合物 - 有机酸及其衍生物 - 羧酸及其衍生物
• 中文名称: L-精氨酸丁酯
• 英文名称: L-Arginin-butylester
• 英文别名: L-Argininbutylester；butyl (2S)-2-amino-5-(diaminomethylideneamino)pentanoate
• CAS: 142382-85-2
• 化学式: C₁₀H₂₂N₄O₂
• 分子量: 230.31
• InChiKey: UQTGZDUYNOOQRP-QMMMGPOBSA-N

物化性质

• 沸点：
  343.7±52.0 °C(Predicted)
• 密度：
  1.20±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -0.3
• 重原子数：
  16
• 可旋转键数：
  9
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.8
• 拓扑面积：
  117
• 氢给体数：
  3
• 氢受体数：
  4

反应信息

• 作为反应物：
  描述：

  L-精氨酸丁酯 、 三氟乙酸酐 生成 butyl (2S)-5-[[N,N'-bis(2,2,2-trifluoroacetyl)carbamimidoyl]-(2,2,2-trifluoroacetyl)amino]-2-[(2,2,2-trifluoroacetyl)amino]pentanoate
  参考文献：
  名称：

  ZUMWALT R. W.; DESGRES J.; KUO K. C.; PAUTZ J. E.; GEHRKE C. W., J. ASSOC. OFFIC.-ANAL. CHEM., 70,(1987) N 2, 253-262

  摘要：

  DOI：
• 作为产物：
  描述：

  L-精氨酸 、 正丁醇 在 盐酸 作用下， 生成 L-精氨酸丁酯
  参考文献：
  名称：

  Weitzel; Renner; Guglielmi, Hoppe-Seyler's Zeitschrift fur Physiologische Chemie, 1971, vol. 352, # 12, p. 1617 - 1630

  摘要：

  DOI：

文献信息

• PROTEIN REFORMING METHOD
  申请人：Ajinomoto Co., Inc.

  公开号：EP2281827A1

  公开（公告）日：2011-02-09

  The present invention provides a method for producing a protein which has restored a higher-order structure of a native state thereof: by bringing the protein which has lost a higher-order structure thereof into contact at pH 6.5 to 9.0 to a 1 to 3% aqueous solution of a specific surfactant, such as lauroylglutamic acid to obtain a solubilized solution of the protein; and adding the obtained solubilized solution to a buffer with pH 6.5 to 9.0 containing arginine or an arginine derivative at a level of 0.1 to 1.2 M to lower the concentration of the specific surfactant, such as lauroylglutamic acid, in an obtained mixture solution down to 0.02 to 0.275%. According to the present invention, it is possible to easily restore a higher-order structure of a native state of a protein while smoothly detaching a surfactant from the protein.

  本发明提供了一种生产恢复了原生态高阶结构的蛋白质的方法：将失去高阶结构的蛋白质在pH6.5至9.0的特定表面活性剂（如月桂酰谷氨酸）的1%至3%的水溶液接触，以获得该蛋白质的增溶溶液；并将所获得的增溶溶液加入pH值为6.5至9.0的缓冲液中，该缓冲液中含有0.1至1.2M的精氨酸或精氨酸衍生物，以将所获得的混合物溶液中的特定表面活性剂（如月桂酰谷氨酸）的浓度降至0.02%至0.275%。根据本发明，可以在使表面活性剂与蛋白质顺利分离的同时，轻松恢复蛋白质原生状态的高阶结构。
• Beneficial effects of increasing local blood flow
  申请人：Strategic Science & Technologies, LLC

  公开号：EP2380579A1

  公开（公告）日：2011-10-26

  The present invention generally relates to the improvement of tissue health by increasing local blood flow. In some aspects of the invention, increased local blood flow is effected by the transdermal delivery of the nitric oxide precursor L-arginine and/or its derivatives alone, or optionally in conjunction with an adjunct such as theophylline. The transdermal delivery is effected, in certain embodiments through the means of a hostile biophysical environment, such as that created by a high ionic strength environment. Various pathological states caused by, or occurring in conjunction with, insufficient blood flow, can be treated using the systems and methods of the invention as described herein. In other embodiments, increased blood flow using the systems and methods of the invention may result in enhanced healing, for example, through greater availability of the constituents of the blood. Examples of conditions which may benefit from increased blood flow include, but are not limited to, erectile dysfunction, hair loss, female sexual dissatisfaction, sagging facial or other body tissue, peripheral vascular disease including claudication, neuropathy, skin ulcers, bone healing, wound healing, viral and bacterial infection, and skin grafting.

  本发明一般涉及通过增加局部血流量来改善组织健康。在本发明的某些方面，一氧化氮前体 L-精氨酸和/或其衍生物单独或与茶碱等辅助剂一起透皮给药可增加局部血流量。在某些实施方案中，透皮给药是通过恶劣的生物物理环境（如高离子强度环境）来实现的。使用本文所述的本发明系统和方法，可以治疗由血流不足引起或与之同时发生的各种病理状态。在其他实施例中，使用本发明的系统和方法增加血流量可提高愈合能力，例如，通过提高血液成分的可用性。可从增加血流量中获益的情况包括但不限于勃起功能障碍、脱发、女性性不满、面部或其他身体组织下垂、外周血管疾病（包括跛行）、神经病、皮肤溃疡、骨愈合、伤口愈合、病毒和细菌感染以及植皮。
• METHOD FOR PREPARING POLYMERIC PROTEIN COMPOSED OF MONOMERIC PROTEIN PRODUCED BY FUSING PROTEIN HAVING IMMUNOGLOBULIN FOLD STRUCTURE TO PROTEIN CAPABLE OF SERVING AS SUBUNIT STRUCTURE
  申请人：Ajinomoto Co., Inc.

  公开号：EP2725104A1

  公开（公告）日：2014-04-30

  The present invention provides a method for producing a multimeric protein consisting of a monomeric protein obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.

  本发明提供了一种生产多聚体蛋白质的方法，该多聚体蛋白质由具有免疫球蛋白折叠结构的蛋白质与可作为亚单位结构的蛋白质融合得到的单体蛋白质组成，该方法包括以下步骤： (A) 在微生物细胞中制备具有不溶性颗粒状的单体蛋白； (B) 用含有月桂酰-L-Glu 的水溶液增溶步骤(A)中制备的单体蛋白； (C) 在含有盐酸精氨酸的缓冲液中稀释步骤(B)中得到的溶液，以降低月桂酰-L-谷氨酰的浓度；以及 (D) 使用凝胶过滤色谱法或类似方法将步骤(C)中得到的溶液的溶剂替换为缓冲液。
• Method for preparing polymeric protein composed of monomeric protein produced by fusing protein having immunoglobulin fold structure to protein capable of serving as subunit structure
  申请人：AJINOMOTO CO., INC.

  公开号：US10308969B2

  公开（公告）日：2019-06-04

  The present invention provides a method for producing a multimeric protein composed of a monomeric protein, wherein the monomeric protein is obtained by fusing a protein having an immunoglobulin fold structure to a protein that can serve as a subunit structure, the method including the steps of: (A) preparing the monomeric protein having an insoluble granular form in cells of a microorganism; (B) solubilizing the monomeric protein prepared in step (A) with an aqueous solution containing lauroyl-L-Glu; (C) diluting a solution obtained in step (B) in a buffer containing arginine hydrochloride to lower a concentration of lauroyl-L-Glu; and (D) replacing a solvent of a solution obtained in step (C) with a buffer using gel filtration chromatography or the like.

  本发明提供了一种生产由单体蛋白质组成的多聚体蛋白质的方法，其中单体蛋白质是通过将具有免疫球蛋白折叠结构的蛋白质与可作为亚单位结构的蛋白质融合而得到的，该方法包括以下步骤： (A) 在微生物细胞中制备具有不溶性颗粒状的单体蛋白； (B) 用含有月桂酰-L-Glu 的水溶液增溶步骤(A)中制备的单体蛋白； (C) 在含有盐酸精氨酸的缓冲液中稀释步骤(B)中得到的溶液，以降低月桂酰-L-谷氨酰的浓度；以及 (D) 使用凝胶过滤色谱法或类似方法将步骤(C)中得到的溶液的溶剂替换为缓冲液。
• Topical and oral delivery of arginine to cause beneficial effects
  申请人：——

  公开号：US20010002405A1

  公开（公告）日：2001-05-31

  The use of orally administered L-arginine in conjunction with a topical preparation for producing enhanced blood flow in tissue thus causing beneficial effects such as warming cold tissue of the hands and feet, promoting hair growth on bald scalp tissue, promoting healing of superficial ulcers such as leg ulcers in persons with diabetes, and overcoming male erectile failure (impotence) is disclosed. Specifically, use of orally administered L-arginine in conjunction with this is topical preparation provides local delivery of the amino acid L-arginine, an important biological precursor to the main substance which is responsible for relaxation of blood vessels permitting enhancement of blood flow. In the preferred embodiments, the L-arginine is provided so that it can be topically applied to the cold tissue. The preparation also contains an agent which aids in the transfer of L-arginine into the tissue. In the preferred embodiments this agent overcomes the resistance to transfer caused by the high charge density of L-arginine. In the preferred embodiments this means is high ionic strength created by addition of sodium chloride. This preparation, when topically applied to cold tissue, warming begins within 10 to 45 minutes and is sustained for periods as long as 2 to 18 hours. Further this preparation when applied nightly to bald scalp tissue for a period of time causes substantial growth of hair on the bald scalp, causes the healing of superficial ulcers such as leg ulcers and overcomes impotence.

  本发明公开了口服 L-精氨酸与外用制剂配合使用的方法，口服 L-精氨酸与外用制剂配合使用可增强组织中的血流量，从而产生有益的效果，如温暖手脚冰冷的组织、促进秃头组织的毛发生长、促进浅表溃疡（如糖尿病患者的腿部溃疡）愈合以及克服男性勃起功能障碍（阳痿）。具体来说，将口服 L-精氨酸与本局部制剂结合使用，可在局部提供氨基酸 L-精氨酸，这是一种重要的生物前体物质，其主要作用是松弛血管，促进血液流动。在优选的实施方案中，提供的 L-精氨酸可局部应用于冷组织。制剂中还含有一种有助于将 L-精氨酸转移到组织中的制剂。在优选的实施方案中，这种制剂可以克服 L-精氨酸的高电荷密度所造成的转移阻力。在优选的实施方案中，这种手段是通过添加氯化钠产生高离子强度。这种制剂局部用于冷组织时，可在 10 至 45 分钟内开始升温，持续时间长达 2 至 18 小时。此外，这种制剂每晚涂抹在秃头组织上一段时间后，可使秃头上的头发大量生长，使浅表溃疡（如腿部溃疡）愈合，并克服阳痿。