FMOC-(S)-3-氨基-4-(3-吡啶基)丁酸 | 270063-60-0

• 物质功能分类: 生物化工 - 氨基酸及其衍生物 - FMOC-氨基酸
• 分子结构分类: 有机化合物 - 苯类化合物 - 芴
• 中文名称: FMOC-(S)-3-氨基-4-(3-吡啶基)丁酸
• 英文名称: (S)-3-((((9H-fluoren-9-yl)methoxy)carbonyl)amino)-4-(pyridin-3-yl)butanoic acid
• 英文别名: (3S)-3-(9H-fluoren-9-ylmethoxycarbonylamino)-4-pyridin-3-ylbutanoic acid
• CAS: 270063-60-0
• 化学式: C₂₀H₂₂NO₅Pol
• mdl: MFCD01861057
• 分子量: 402.4
• InChiKey: QBMDHZYUTBFELW-KRWDZBQOSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.5
• 重原子数：
  30
• 可旋转键数：
  8
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.208
• 拓扑面积：
  88.5
• 氢给体数：
  2
• 氢受体数：
  5

安全信息

• 危险等级：
  IRRITANT
• 危险性防范说明：
  P264,P280,P302+P352,P337+P313,P305+P351+P338,P362+P364,P332+P313
• 危险性描述：
  H315,H319

反应信息

• 作为反应物：
  描述：

  FMOC-(S)-3-氨基-4-(3-吡啶基)丁酸 、 FMOC-(S)-3-氨基-4-(3-吡啶基)丁酸 、 、 、 Fmoc-L-beta-高缬氨酸 、 Fmoc-L-beta-高异亮氨酸 、 (S)-6-Amino-3-(9H-fluoren-9-ylmethoxycarbonylamino)-hexanoic acid 在 哌啶 、 1,8-二氮杂双环[5.4.0]十一碳-7-烯 、 benzotriazol-1-yloxyl-tris-(pyrrolidino)-phosphonium hexafluorophosphate 、 1-羟基苯并三唑 、 N,N-二异丙基乙胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 0.23h， 生成
  参考文献：
  名称：

  Identification of a β3-peptide HIV fusion inhibitor with improved potency in live cells

  摘要：

  We recently reported a beta(3)-decapeptide, beta WWI-1, that binds a validated gp41 model in vitro and inhibits gp41-mediated fusion in cell culture. Here we report six analogs of beta WWI-1 containing a variety of non-natural side chains in place of the central tryptophan of the WWI-epitope. These analogs were compared on the basis of both gp41 affinity in vitro and fusion inibition in live, HIV-infected cells. One new beta(3)-peptide, beta WXI-a, offers a significantly improved CC50/EC50 ratio in the live cell assay. (C) 2009 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.bmcl.2009.05.032

文献信息

• Substrates for detection of botulinum neurotoxin
  申请人：CITY OF HOPE

  公开号：US10775366B2

  公开（公告）日：2020-09-15

  Provided herein is a large immuno-sorbent surface area assay (ALISSA) for the rapid and sensitive detection of botulinum neurotoxins (BoNTs) and anthrax toxin. This assay is designed to capture a low number of toxin molecules and to measure their intrinsic protease activity via conversion of a fluorogenic or luminescent substrate. Also provided herein are novel peptides that can be specifically cleaved by BoNT and novel peptides that are resistant to cleavage by BoNT. The combination of these cleavable and control peptides can be used for implementation of an exemplary ALISSA used to specifically detect BoNT enzymatic activity. Furthermore, the ALISSA as described herein may also be used in a column based format for use in a high-throughput system for testing large quantities of samples.

  本文提供了一种大型免疫吸附表面积测定法（ALISSA），用于快速灵敏地检测肉毒杆菌神经毒素（BoNTs）和炭疽毒素。这种检测方法设计用于捕获少量毒素分子，并通过荧光或发光底物的转化来测量其内在蛋白酶活性。本文还提供了可被 BoNT 特异性裂解的新型多肽和抗 BoNT 裂解的新型多肽。这些可裂解肽和对照肽的组合可用于实施一种用于特异性检测 BoNT 酶活性的 ALISSA 示例。此外，本文所述的 ALISSA 还可采用柱式格式，用于检测大量样品的高通量系统。
• METHODS FOR DETECTION OF BOTULINUM NEUROTOXIN
  申请人：Kalkum Markus

  公开号：US20120322081A1

  公开（公告）日：2012-12-20

  Provided herein is a large immuno-sorbent surface area assay (ALISSA) for rapid and sensitive detection of toxin or enzyme activity. This assay is designed to capture a low number of toxin or enzyme molecules and to measure their intrinsic protease activity via conversion of a fluorigenic or luminescent substrate. The ALISSA is significantly faster and more sensitive than methods routinely utilized in the art. This assay is applicable for use for detection of a variety of toxins or enzymes having proteolytic activity, such as botulinum neurotoxin, bacillus anthracis lethal factor, human chitinases, and aspergillus fumigatus proteases. Also provided are methods for constructing and identifying novel luminescent or fluorescent substrates suitable for use with the ALISSA method.