photoproduct repair at a thymine–cytosine sequence. A model compound formed by photocycloaddition between thymine and 6‐azauracil has been designed to mimic the elusive azetidine intermediate. The photoinduced electron transfer process has been investigated by means of steady‐state and time‐resolved fluorescence using photosensitizers with oxidation potentials in the singlet excited state ranging from −3.3
光解酶是一种有趣的酶,可以利用阳光来恢复病变,例如
环丁烷嘧啶二聚体或(6-4)光产物。这项工作的重点是负责在胸腺
嘧啶-
胞嘧啶序列的(6-4)光产物修复过程中发生的氮杂
环丁烷环分裂的光还原过程。在胸腺
嘧啶和6-氮杂尿
嘧啶之间通过光环加成反应形成的模型化合物已被设计为模仿难以捉摸的氮杂
环丁烷中间体。已通过使用光敏剂对稳态和时间分辨荧光进行了光诱导电子转移过程的研究,光敏剂的单重激发态氧化电位相对于SCE为-3.3至-2.1V。氮杂
环丁烷环的分裂和“修复”碱基的回收已通过HPLC分析证实。