N-[2-[2-(2-(2-methylacryloylamino)ethoxy)ethoxy]ethyl]-2-methylacrylamide | 1005456-54-1

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: N-[2-[2-(2-(2-methylacryloylamino)ethoxy)ethoxy]ethyl]-2-methylacrylamide
• 英文别名: (ethylenedioxy)bis(ethylamine)；Methyl 4-[2-[2-[2-(2-methylprop-2-enoylamino)ethoxy]ethoxy]ethylamino]-4-oxobutanoate
• CAS: 1005456-54-1
• 化学式: C₁₅H₂₆N₂O₆
• 分子量: 330.381
• InChiKey: IVVFOSZSAGWYKG-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.7
• 重原子数：
  23
• 可旋转键数：
  14
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  103
• 氢给体数：
  2
• 氢受体数：
  6

反应信息

• 作为产物：
  描述：

  1,8-二氨基-3,6-二氧杂辛烷 在 盐酸-N-乙基-Nˊ-(3-二甲氨基丙基)碳二亚胺 作用下， 以 氯仿 为溶剂， 反应 16.0h， 生成 N-[2-[2-(2-(2-methylacryloylamino)ethoxy)ethoxy]ethyl]-2-methylacrylamide
  参考文献：
  名称：

  Sugar chain-capturing substance and use thereof

  摘要：

  本发明提供了一种制备样品的方法，其特征在于将含有肼基的物质A与糖链和/或糖衍生物通过肼酮化反应结合，从而使得能够通过简单操作从含有糖链和/或糖衍生物的生物样品中分离和纯化用于分析的糖链和/或糖衍生物。

  公开号：

  US09340651B2

文献信息

• Comprehensive Approach to Structural and Functional Glycomics Based on Chemoselective Glycoblotting and Sequential Tag Conversion
  作者：Jun-ichi Furukawa、Yasuro Shinohara、Hiromitsu Kuramoto、Yoshiaki Miura、Hideyuki Shimaoka、Masaki Kurogochi、Mika Nakano、Shin-Ichiro Nishimura

  DOI：10.1021/ac702124d

  日期：2008.2.1

  Changes in protein glycosylation profoundly affect protein function. To understand these effects of altered protein glycosylation, we urgently need high-throughput technologies to analyze glycan expression and glycan−protein interactions. Methods are not available for amplification of glycans; therefore, highly efficient sample preparation is a major issue. Here we present a novel strategy that allows flexible and sequential incorporation of various functional tags into oligosaccharides derived from biological samples in a practical manner. When combined with a chemoselective glycoblotting platform, our analysis enables us to complete sample preparation (from serum to released, purified, methyl-esterified, and labeled glycans) in 8 h from multiple serum samples (up to 96 samples) using a 96-well microplate format and a standard de-N-glycosylation protocol that requires reductive alkylation and tryptic digestion prior to PNGase F digestion to ensure maximal de-N-glycosylation efficiency. Using this technique, we quantitatively detected more than 120 glycans on human carcinoembryonic antigens for the first time. This approach was further developed to include a streamlined method of purification, chromatographic fractionation, and immobilization onto a solid support for interaction analysis. Since our approach enables rapid, flexible, and highly efficient tag conversion, it will contribute greatly to a variety of glycomic studies.

  蛋白质糖基化的变化会深刻影响蛋白质的功能。为了了解蛋白质糖基化变化的这些影响，我们迫切需要高通量技术来分析聚糖表达和聚糖-蛋白质相互作用。目前还没有扩增聚糖的方法，因此高效的样品制备是一个主要问题。在这里，我们提出了一种新颖的策略，能以实用的方式灵活、有序地将各种功能标签整合到从生物样本中提取的寡糖中。当与化学选择性糖印迹平台结合使用时，我们的分析能让我们在 8 小时内完成多个血清样本（多达 96 个样本）的样本制备（从血清到释放、纯化、甲基酯化和标记的糖），使用的是 96 孔微孔板格式和标准的去 N-糖基化方案，该方案要求在 PNGase F 消化之前进行还原烷基化和胰蛋白酶消化，以确保最大的去 N-糖基化效率。利用这种技术，我们首次定量检测了人类癌胚抗原上的 120 多个聚糖。我们进一步开发了这种方法，包括简化的纯化、色谱分馏和固定到固体支持物上进行相互作用分析的方法。由于我们的方法能够实现快速、灵活和高效的标签转换，它将极大地促进各种糖化学研究。
• SUGAR CHAIN-CAPTURING SUBSTANCE AND USE THEREOF
  申请人：Sumitomo Bakelite Company, Ltd.

  公开号：EP2056106A1

  公开（公告）日：2009-05-06

  The present invention provides a method for preparing a sample characterized by binding a substance A containing a hydrazide group to a sugar chain and/or a sugar derivative via hydrazone formation between the hydrazide group of the substance A and the reducing end of the sugar chain and/or the sugar derivative thereby to enable the separation and purification of the sugar chain and/or the sugar derivative for an analytical sample from a biological sample containing the sugar chain and/or the sugar derivative by a simple operation.

  本发明提供了一种制备样品的方法，其特征在于通过在物质 A 的酰肼基团与糖链和/或糖衍生物的还原端之间形成腙，将含有酰肼基团的物质 A 与糖链和/或糖衍生物结合，从而能够通过简单的操作从含有糖链和/或糖衍生物的生物样品中分离和纯化用于分析样品的糖链和/或糖衍生物。
• Sugar chain- capturing substance and use thereof
  申请人：Sumitomo Bakelite Company, Ltd.

  公开号：EP2518494A2

  公开（公告）日：2012-10-31

  The present invention provides a method for preparing a sample characterized by binding a substance A containing a hydrazide group to a sugar chain and/or a sugar derivative via hydrazone formation between the hydrazide group of the substance A and the reducing end of the sugar chain and/or the sugar derivative thereby to enable the separation and purification of the sugar chain and/or the sugar derivative for an analytical sample from a biological sample containing the sugar chain and/or the sugar derivative by a simple operation.

  本发明提供了一种制备样品的方法，其特征在于通过在物质 A 的酰肼基团与糖链和/或糖衍生物的还原端之间形成腙，将含有酰肼基团的物质 A 与糖链和/或糖衍生物结合，从而能够通过简单的操作从含有糖链和/或糖衍生物的生物样品中分离和纯化用于分析样品的糖链和/或糖衍生物。
• COMPOSITION, METHOD FOR PREPARING SUGAR CHAIN SAMPLE, AND METHOD FOR ANALYZING SUGAR CHAIN
  申请人：Sumitomo Bakelite Co.,Ltd.

  公开号：EP3096136A1

  公开（公告）日：2016-11-23

  A composition is provided, including: polymer particles having a functional group for capturing a sugar chain, wherein the rate of change of mass calculated by Equation (F1) below is more than 90% and less than 112%. Rate of change%=A/B×100 [In Equation (F1), A represents the mass of the composition after incubation for 4 hours under an environment of a relative humidity of 50% and a temperature of 25°C, and B represents the mass of the composition before the incubation.

  本发明提供了一种组合物，包括：具有用于捕获糖链的官能团的聚合物颗粒，其中按以下公式（F1）计算的质量变化率大于 90%，小于 112%。变化率%=A/B×100 [在公式（F1）中，A 表示在相对湿度为 50%、温度为 25°C 的环境下培养 4 小时后组合物的质量，B 表示培养前组合物的质量。
• SUGAR CHAIN ARRAY
  申请人：Sumitomo Bakelite Co., Ltd.

  公开号：EP2600152B1

  公开（公告）日：2017-01-25