申请人:McConnell Ivan Robert
公开号:US20060223132A1
公开(公告)日:2006-10-05
The present invention relates to a method for preparing haptens of formulae I and II that are useful in the preparation of immunogens, antibodies and conjugates, for use in competitive immunoassays for the detection of ractopamine, isoxsuprine and ritodrine. The haptens are prepared by reacting a phenylethanolamine derivative of formula D with a phenylalkylcarbonyl derivative of formula E:
in which, in formula I, Z
1
is a crosslinker and Z
2
is H and, in formula II, Z
1
is H and Z
2
is a crosslinker.
本发明涉及一种制备公式I和II的半抗原的方法,该半抗原在制备免疫原、抗体和结合物中有用,用于竞争性免疫测定检测莱克多巴胺、异丝氨酮和利托腈。半抗原通过将公式D的苯乙醇胺衍生物与公式E的苯基烷基羧酰衍生物反应制备而成。在公式I中,Z1是交联剂,Z2是氢,在公式II中,Z1是氢,Z2是交联剂。