Composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid and their derivatives and method of preparation thereof
申请人:MALAYSIAN PALM OIL BOARD
公开号:US10603348B2
公开(公告)日:2020-03-31
A composition comprising caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives extracted from any part of oil palm including but not confined to the vegetation liquor of palm oil milling and palm oil mill effluent, and a method for use in the preparation of a composition containing caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives. The method includes the steps of pre-concentrating an extract containing the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, caffeoylshikimic acids and their derivatives and isolating the caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, said caffeoylshikimic acids and their derivatives from said extract by liquid chromatography, wherein the elution activity of said caffeoylshikimic acids, protocatechuic acid, hydroxytyrosol, hydroxybenzoic acid, with the caffeoylshikimic acids and their derivatives varying depending on the stationary phase and the composition of the mobile phase.
一种包含咖啡酰
莽草酸、
原儿茶酸、
羟基酪醇、羟基
苯甲酸的组合物,所述咖啡酰
莽草酸及其衍
生物提取自油棕的任何部分,包括但不限于棕榈油碾磨的植液和棕榈油厂的污
水、以及一种用于制备含有咖啡酰
莽草酸、
原儿茶酸、
羟基酪醇、羟基
苯甲酸、所述咖啡酰
莽草酸及其衍
生物的组合物的方法。该方法包括以下步骤:预浓缩含有咖啡
酰亚胺酸、
原儿茶酸、
羟基酪醇、羟基
苯甲酸、咖啡
酰亚胺酸及其衍
生物的
提取物,并分离咖啡
酰亚胺酸、
原儿茶酸、
羟基酪醇、羟基
苯甲酸、用
液相色谱法从所述
提取物中分离出所述咖啡酰
莽草酸及其衍
生物,其中所述咖啡酰
莽草酸、
原儿茶酸、
羟基酪醇、羟基
苯甲酸与咖啡酰
莽草酸及其衍
生物的洗脱活性随固定相和流动相组成的不同而变化。