申请人:Verenium Corporation
公开号:EP2404930A1
公开(公告)日:2012-01-11
The invention relates to polypeptides having glucanase, eg endoglucanase, mannanase, xylanase activity or a combination of these activities, and polynucleotides encoding them. In one aspect, the glucanase activity is an endoglucanase activity (eg, endo-1,4-beta-D-glucan 4-glucano hydrolase activity) and comprises hydrolysis of 1,4-beta-D-glycosidic linkages in cellulose, cellulose derivatives (eg carboxy methyl cellulose and hydroxy ethyl cellulose) lichenin, beta-1,4-bonds in mixed beta-1,3-glucans, such as cereal beta-D-glucans or xyloglucans and other plant material containing cellulosic parts. In addition, methods of designing new enzymes and methods of use thereof are also provided. In alternative aspects, the new glucanases eg, endoglucanases, mannanases, xylanases have increased activity and stability at increased pH and temperature.
本发明涉及具有葡聚糖酶(如内切葡聚糖酶、甘露聚糖酶、木聚糖酶)活性或这些活性组合的多肽,以及编码它们的多核苷酸。在一个方面,葡聚糖酶活性是内切葡聚糖酶活性(例如,内切-1,4-beta-D-葡聚糖 4-葡聚糖水解酶活性),包括水解纤维素中的 1,4-beta-D-糖苷键、纤维素衍生物(如羧甲基纤维素和羟乙基纤维素)、地衣素、混合 β-1,3-葡聚糖中的β-1,4-键(如谷物中的β-D-葡聚糖或木糖)以及其他含纤维素 部分的植物材料中的 1,4-β-D-糖苷键。此外,还提供了设计新酶的方法及其使用方法。在其他方面,新的葡聚糖酶,例如内切葡聚糖酶、甘露聚糖酶、木聚糖酶在pH值和温度升高的情况下具有更高的活性和稳定性。