Β-羟基丁酸脱氢酶 | 9028-38-0

• 物质功能分类: 食品添加剂 - 酶制剂
• 分子结构分类: 氧化还原酶 - 作用于CH-OH供体组 - 以NAD+或NADP+为受体
• 中文名称: Β-羟基丁酸脱氢酶
• 中文别名: Β-羟丁酸脱氢酶；D-3-羟基丁酸酯,脱氢酶；3-羟基丁酸脱氢酶；β-羟丁酸脱氢酶来源于球形红假单胞菌；D-3-羟丁酸脱氢酶；3-羟基丁酸脱氢酶(红假单胞球菌)；Β-羟丁酸脱氢酶/D-3-羟丁酸脱氢酶/3-羟基丁酸脱氢酶/Β-羟基丁酸脱氢酶/D-3-羟基丁酸酯脱氢酶；Β-羟丁酸脱氢酶,400单位/毫克
• 英文名称: 3-hydroxybutyrate dehydrogenase
• 英文别名: NAD-beta-hydroxybutyrate dehydrogenase；hydroxybutyrate oxidoreductase；beta-hydroxybutyrate dehydrogenase；D-beta-hydroxybutyrate dehydrogenase；D-3-hydroxybutyrate dehydrogenase；D-(-)-3-hydroxybutyrate dehydrogenase；beta-hydroxybutyric acid dehydrogenase；3-D-hydroxybutyrate dehydrogenase；beta-hydroxybutyric dehydrogenase
• CAS: 9028-38-0

物化性质

• 稳定性/保质期：

  Application Suitable for the determination of acetoacetate and D(-)-3-hydroxybutyrate by the method of Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974).

  Biochem/physiol Actions In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Pseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is essential for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in converting ketone bodies into citric acid, which is further metabolized via the tricarboxylic acid cycle (Krebs cycle).

  Unit Definition One unit will oxidize 1.0 μmole of D-β-hydroxybutyrate to acetoacetate per minute at pH 7.8 and 37 °C.

  Physical form Lyophilized powder containing sucrose and Tris buffer salts.

安全信息

• 危险品标志：
  Xi
• 安全说明：
  S24/25,S26,S36
• 危险类别码：
  R36/37/38
• WGK Germany：
  3
• 海关编码：
  35079090

制备方法与用途

简介

3-羟基丁酸脱氢酶也称为“D-3-羟基丁酸脱氢酶”或“D-3-羟丁酸脱氢酶”，是一种以NAD+或NADP+为受体、作用于供体CH-OH基团上的氧化还原酶。这种酶能催化以下酶促反应：

(R)-3-羟基丁酸 + NAD+ → 乙酰乙酸 + NADH + H+

应用

3-羟基丁酸脱氢酶主要参与酮类物质的合成与降解以及丁酸的代谢过程。此外，这种酶还能氧化3-羟基单羧酸。

用途

该酶适用于通过Williamson, D.H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍的方法测定乙酰乙酸酯和D(-)-3-羟基丁酸酯。