乙醇脱氢酶(NADP) | 9028-12-0

• 分子结构分类: 氧化还原酶 - 作用于CH-OH供体组 - 以NAD+或NADP+为受体
• 中文名称: 乙醇脱氢酶(NADP)
• 中文别名: 乙醇脱氢酶
• 英文名称: alcohol dehydrogenase (NADP+)
• 英文别名: aldehyde reductase (NADPH2)；NADP-alcohol dehydrogenase；NADP+-aldehyde reductase；NADP+-dependent aldehyde reductase；NADPH-aldehyde reductase；NADPH-dependent aldehyde reductase；nonspecific succinic semialdehyde reductase；ALR 1；low-Km aldehyde reductase；high-Km aldehyde reductase；alcohol dehydrogenase (NADP)；recombinant ketoreductase
• CAS: 9028-12-0

反应信息

• 作为试剂：
  描述：

  乙酰乙酸乙酯 在 还原型辅酶II(NADPH)四钠盐 葡萄糖 、 D-glucose dehydrogenase 、 乙醇脱氢酶(NADP) 作用下， 以 phosphate buffer 为溶剂， 生成 (R)-(-)-3-羟基丁酸乙酯 、 3-羟基丁酸乙酯
  参考文献：
  名称：

  A recombinant ketoreductase tool-box. Assessing the substrate selectivity and stereoselectivity toward the reduction of β-ketoesters

  摘要：

  The substrate selectivity and stereoselectivity of a series of ketoreductases were evaluated toward the reduction of two sets of beta-ketoesters. Both the structural variety at beta-position and the substituent at alpha-position greatly affected the activity and stereoselectivity of these ketoreductases. For the first set of beta-ketoesters, at least one ketoreductase was found that catalyzed the formation of either (D) or (L) enantiomer of beta-hydroxyesters from each substrate with high optical purity, with the only exception of ethyl (D)-3-hydroxy3-phenylpropionate. For the second set of beta-ketoesters with alpha-substituents, the situation is more complex. More commonly, a ketoreductase was found that formed one of the four diastereomers in optically pure form, with only a few cases in which enzymes could be found that formed two or more of the diastereomers in high optical purity. The continued development of new, more diverse ketoreductases will create the capability to produce a wider range of single diastereomers of 2-substituted-3-hydroxy acids and their derivatives. (c) 2005 Elsevier Ltd. All rights reserved.

  DOI：

  10.1016/j.tet.2005.10.044