cis-3-Ethyl-3,5-cyclohexadiene-1,2-diol

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: cis-3-Ethyl-3,5-cyclohexadiene-1,2-diol
• 英文别名: (1R,2S)-3-ethylcyclohexa-3,5-diene-1,2-diol
• 化学式: C₈H₁₂O₂
• 分子量: 140.182
• InChiKey: BACDCBUEYBFLFV-SFYZADRCSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  0.3
• 重原子数：
  10
• 可旋转键数：
  1
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  40.5
• 氢给体数：
  2
• 氢受体数：
  2

反应信息

• 作为反应物：
  描述：

  cis-3-Ethyl-3,5-cyclohexadiene-1,2-diol 在 高氯酸 作用下， 生成 乙基苯酚
  参考文献：
  名称：

  Acid-catalysed aromatisation of benzene cis-1,2-dihydrodiols: a carbocation transition state poorly stablised by resonance

  摘要：

  酸催化下3-取代苯顺-1,2-二氢二醇的脱水反应呈现出ρ=–8.2的哈米特图，这与通过类似苯镝离子中间体的反应途径一致；然而，通过σp而非σ+常数关联甲基和甲氧基等+M共振取代基，表明过渡态的共振与诱导稳定之间存在明显失衡。

  DOI：

  10.1039/c39940000313

文献信息

• Probe compound for detecting and isolating enzymes and means and methods using the same
  申请人：Helmholtz-Zentrum für Infektionsforschung GmbH

  公开号：EP2230312A1

  公开（公告）日：2010-09-22

  The present invention relates to a probe compound that can comprise any substrate or metabolite of an enzymatic reaction in addition to an indicator component, such as, for example, a fluorescence dye, or the like. Moreover, the present invention relates to means for detecting enzymes in form of an array, which comprises any number of probe compounds of the invention which each comprise a different metabolite of interconnected metabolites representing the central pathways in all forms of life. Moreover, the present invention relates to a method for detecting enzymes involving the application of cell extracts or the like to the array of the invention which leads to reproducible enzymatic reactions with the substrates. These specific enzymatic reactions trigger the indicator (e.g. a fluorescence signal) and bind the enzymes to the respective cognate substrates. Moreover, the invention relates to means for isolating enzymes in form of nanoparticles coated with the probe compound of the invention. The immobilisation of the cognate substrates or metabolites on the surface of nanoparticles by means of the probe compounds allows capturing and isolating the respective enzyme, e.g. for subsequent sequencing.

  本发明涉及一种探针化合物，它可以包括酶反应的任何底物或代谢物，此外还包括指示成分，例如荧光染料或类似物。此外，本发明还涉及以阵列形式检测酶的方法，该阵列由任意数量的本发明探针化合物组成，每种探针化合物由代表所有生命形式中中心途径的相互关联的代谢物中的不同代谢物组成。此外，本发明还涉及一种检测酶的方法，该方法涉及将细胞提取物或类似物应用于本发明的阵列，从而导致与底物发生可重复的酶反应。这些特定的酶反应会触发指示剂（如荧光信号），并将酶与各自的同源底物结合。此外，本发明还涉及以涂覆有本发明探针化合物的纳米颗粒形式分离酶的方法。通过探针化合物将同源底物或代谢物固定在纳米颗粒表面，可以捕获和分离相应的酶，例如用于后续测序。
• Methods for conducting assays for enzyme activity on protein microarrays
  申请人：Zhou X. Fang

  公开号：US20050118665A1

  公开（公告）日：2005-06-02

  The present invention relates to methods of conducting assays for enzymatic activity on microarrays useful for the large-scale study of protein function, screening assays, and high-throughput analysis of enzymatic reactions. The invention relates to methods of using protein chips to assay the presence, amount, activity and/or function of enzymes present in a protein sample on a protein chip. In particular, the methods of the invention relate to conducting enzymatic assays using a microarray wherein a protein and a substance are immobilized on the surface of a solid support and wherein the protein and the substance are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the substance and the protein. The invention also relates to microarrays that have an enzyme and a substrate immobilized on their surface wherein the enzyme and the substrate are in proximity to each other sufficient for the occurrence of an enzymatic reaction between the enzyme and the substrate.

  本发明涉及在微阵列上进行酶活性测定的方法，这种方法可用于蛋白质功能的大规模研究、筛选测定和酶反应的高通量分析。本发明涉及使用蛋白质芯片检测蛋白质芯片上蛋白质样品中酶的存在、数量、活性和/或功能的方法。特别是，本发明的方法涉及使用芯片进行酶测定，其中蛋白质和物质被固定在固体支持物表面，蛋白质和物质相互靠近，足以使物质和蛋白质之间发生酶反应。本发明还涉及在其表面固定有酶和底物的微阵列，其中酶和底物相互靠近，足以使酶和底物之间发生酶反应。
• Nucleic Acids and Proteins and Methods for Making and Using Them
  申请人：Mathur Eric J.

  公开号：US20100011456A1

  公开（公告）日：2010-01-14

  The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs.
• NUCLEIC ACIDS AND PROTEINS AND METHODS FOR MAKING AND USING THEM
  申请人：Chang Hwai

  公开号：US20110296543A1

  公开（公告）日：2011-12-01

  The invention provides polypeptides, including enzymes, structural proteins and binding proteins, polynucleotides encoding these polypeptides, and methods of making and using these polynucleotides and polypeptides. Polypeptides, including enzymes and antibodies, and nucleic acids of the invention can be used in industrial, experimental, food and feed processing, nutritional and pharmaceutical applications, e.g., for food and feed supplements, colorants, neutraceuticals, cosmetic and pharmaceutical needs. Polypeptides of the invention can be used in food processing, brewing, bath additives, alcohol production, peptide synthesis, enantioselectivity, hide preparation in the leather industry, waste management and animal degradation, silver recovery in the photographic industry, medical treatment, silk degumming, biofilm degradation, biomass conversion to a biofuel (e.g., a bioethanol, biomethanol, biopropanol or biobutanol, a biodiesel, etc.), biodefense, antimicrobial agents and disinfectants, personal care and cosmetics, biotech reagents, in corn wet milling and pharmaceuticals such as digestive aids and anti-inflammatory (anti-phlogistic) agents.
• US5093514A
  申请人：——

  公开号：US5093514A

  公开（公告）日：1992-03-03