Cobalt containing reagents and methods for the determination of analytes in aqueous fluids
申请人:EASTMAN KODAK COMPANY
(a New Jersey corporation)
公开号:EP0255089A2
公开(公告)日:1988-02-03
Reagents and methods for the determination of analytes are disclosed, using water soluble cobalt(III) complexes and metallizable dyes to form cobalt(III) complexes of the metallizable dyes. Analytes which can be determined include enzymes, cells and biological reductants, such as NADH, NADPH and ascorbate. Amplification is provided since one equivalent of the analyte provides more than one equivalent of the detectable species.
公开了测定分析物的试剂和方法,使用水溶性钴(III)络合物和可金属化染料,形成可金属化染料的钴(III)络合物。可测定的分析物包括酶、细胞和生物还原剂,如 NADH、NADPH 和抗坏血酸。由于一个当量的分析物可以提供一个以上当量的可检测物种,因此可以提供放大作用。