2-(2-苯并噻唑基)-3,5-二苯基溴化四氮唑 | 55699-64-4

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 唑类
• 中文名称: 2-(2-苯并噻唑基)-3,5-二苯基溴化四氮唑
• 中文别名: 2-(2-苯并噻唑)-3,5-二苯基溴化四氮唑；2-(2-苯并噻唑) -3,5-二苯基溴化四氮唑
• 英文名称: 3-(benzo[d]thiazol-2-yl)-2,5-diphenyltetrazol-3-ium bromide
• 英文别名: 2-(Benzthiazolyl-(2))-3,5-diphenyl-tetrazoliumbromid；2-(2-Benzothiazolyl)-3,5-diphenyltetrazolium Bromide；2-(3,5-diphenyltetrazol-2-ium-2-yl)-1,3-benzothiazole;bromide
• CAS: 55699-64-4
• 化学式: Br*C₂₀H₁₄N₅S
• 分子量: 436.335
• InChiKey: BSDDIVAXZYQIKN-UHFFFAOYSA-M

计算性质

• 辛醇/水分配系数(LogP)：
  0.82
• 重原子数：
  27
• 可旋转键数：
  3
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  75.7
• 氢给体数：
  0
• 氢受体数：
  5

反应信息

• 作为产物：
  描述：

  Benzothiazole, 2-[[phenyl(phenylazo)methylene]hydrazino]-, (Z,E)- 在 N-溴代丁二酰亚胺(NBS) 作用下， 以 乙酸乙酯 为溶剂， 20.0 ℃ 、101.33 kPa 条件下， 以48 %的产率得到2-(2-苯并噻唑基)-3,5-二苯基溴化四氮唑
  参考文献：
  名称：

  用于在哺乳动物细胞中释放抗增殖甲臜螯合剂的四唑阳离子的设计

  摘要：

  癌细胞通常对铁有较高的需求，铁在肿瘤进展和转移中起着至关重要的作用。这种铁成瘾为开发针对铁代谢的广谱抗癌药物提供了机会。在这种情况下，研究了预螯合方法，以在特定条件下释放金属结合化合物，从而限制脱靶毒性。在这里，我们展示了一种受广泛用于评估哺乳动物细胞活力的四唑阳离子生物还原启发的预螯合策略。我们设计了一系列基于四唑的化合物，用于细胞内释放金属结合甲臜配体。适合细胞内还原的还原电位和甲臜支架上的N-吡啶基供体的组合产生了两种有效的前螯合剂。还原的甲臜作为三齿配体结合，并稳定配体与金属化学计量比为 2:1 的复合物中的低自旋 Fe(II) 中心。四唑盐在血清中稳定超过 24 小时，并且在一组癌细胞系中记录了微摩尔水平的抗增殖活性。其他测定证实了前螯合剂的细胞内激活及其影响细胞周期进程、诱导细胞凋亡和干扰铁可用性的能力。前螯合剂影响了关键铁调节因子（即转铁蛋白受体 1 和铁蛋白）的表达水平，并

  DOI：

  10.1021/jacs.3c02033

文献信息

• Method of colorimetry and reagent for use therein
  申请人：——

  公开号：US20040142406A1

  公开（公告）日：2004-07-22

  The present invention provides a calorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

  本发明提供了一种热量计法，可以在短时间内进行简单可靠的分析。该方法包括使用氧化还原酶将电子从待分析物转移至染色试剂，通过介质还原产生颜色，并通过测量染色试剂中产生的颜色进行待分析物的定性或定量分析。该方法的酶反应是单阶段反应，颜色产生反应通过介质进行。因此，可在短时间内进行测量。由于该反应既不需要过氧化氢也不需要氧气，因此测量结果非常可靠。
• METHOD OF COLORIMETRY AND REAGENT FOR USE THEREIN
  申请人：ARKRAY, Inc.

  公开号：EP1457572A1

  公开（公告）日：2004-09-15

  The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

  本发明提供了一种比色法，可在短时间内进行简单可靠的分析。该方法包括利用氧化还原酶将电子从被分析物转移到通过介质还原产生颜色的显色试剂中；以及通过测量显色试剂中产生的颜色对被分析物进行定性或定量分析。该方法中的酶反应是一个单级反应，而颜色生成反应是通过介质发生的。因此，测量可在短时间内完成。由于该反应既不需要过氧化氢，也不需要氧气，因此测量值非常可靠。
• Colorimetric method and reagent used for the same
  申请人：Arkray, Inc.

  公开号：EP1942195A1

  公开（公告）日：2008-07-09

  The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.

  本发明提供了一种比色法，可在短时间内进行简单可靠的分析。该方法包括利用氧化还原酶将电子从被分析物转移到通过介质还原产生颜色的显色试剂中；以及通过测量显色试剂中产生的颜色对被分析物进行定性或定量分析。该方法中的酶反应是一个单级反应，而颜色生成反应是通过介质发生的。因此，测量可在短时间内完成。由于该反应既不需要过氧化氢，也不需要氧气，因此测量值非常可靠。
• ELECTROPHORESIS CHIP, ELECTROPHORESIS DEVICE, AND SAMPLE ANALYZING METHOD BY CAPILLARY ELECTROPHORESIS METHOD
  申请人：National Institute of Advanced Industrial Science and Technology

  公开号：EP2148193A1

  公开（公告）日：2010-01-27

  An electrophoresis chip that can be small and simple and that can analyze a sample with high accuracy is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, an introduction reservoir 2a, a recovery reservoir 2b and a capillary channel for sample analysis 3x. The introduction reservoir 2a and the recovery reservoir 2b are formed in the lower substrate 1. The introduction reservoir 2a and the recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x. The introduction reservoir 2a receives a sample to be measured. The sample is electrophoretically introduced directly into the capillary channel for sample analysis 3x by creating a potential difference between the introduction reservoir 2a and the recovery reservoir 2b, and is also analyzed in the capillary channel for sample analysis 3x during the separation of the sample while the sample is being continuously supplied.

  本发明提供了一种体积小、结构简单并能对样品进行高精度分析的电泳芯片。电泳芯片包括上基板 4、下基板 1、导入槽 2a、回收槽 2b 和用于样品分析的毛细管通道 3x。 导入槽 2a 和回收槽 2b 形成于下基板 1 中。导入贮液器 2a 和回收贮液器 2b 通过用于样品分析的毛细管通道 3x 相互连通。导入槽 2a 接收待测样品。 通过在引入贮液器 2a 和回收贮液器 2b 之间产生电位差，以电泳方式将样品直接引入用于样品分析的毛细管通道 3x，并在连续供应样品的过程中，在用于样品分析的毛细管通道 3x 中对样品进行分析。
• ANALYSIS CHIP AND ANALYSIS APPARATUS
  申请人：Arkray, Inc.

  公开号：EP2144057A1

  公开（公告）日：2010-01-13

  An analysis chip that enables an apparatus to be small, analysis to be simple, analysis time to be short and analysis of both glycosylated hemoglobin and glucose to be highly accurate is provided. The electrophoresis chip includes an upper substrate 4, a lower substrate 1, a first introduction reservoir 2a, a first recovery reservoir 2b and a capillary channel for sample analysis 3x; the first introduction reservoir 2a and the first recovery reservoir 2b are formed in the lower substrate 1; and the first introduction reservoir 2a and the first recovery reservoir 2b are in communication with each other via the capillary channel for sample analysis 3x.

  本发明提供了一种能使仪器体积小、分析简单、分析时间短、糖化血红蛋白和葡萄糖分析精度高的分析芯片。该电泳芯片包括上基板4、下基板1、第一引入槽2a、第一回收槽2b和用于样品分析的毛细管通道3x；第一引入槽2a和第一回收槽2b形成于下基板1中；第一引入槽2a和第一回收槽2b通过用于样品分析的毛细管通道3x相互连通。

表征谱图