dGTP(α-S) | 82337-56-2

• 分子结构分类: 有机化合物 - 核苷、核苷酸和类似物 - 嘌呤核苷
• 英文名称: dGTP(α-S)
• 英文别名: [[(2R,3S,5R)-5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-sulfanylphosphoryl] phosphono hydrogen phosphate
• CAS: 82337-56-2
• 化学式: C₁₀H₁₆N₅O₁₂P₃S
• 分子量: 523.251
• InChiKey: IOCRYHATDKHWPM-UMURPWKOSA-N

物化性质

• 密度：
  2.56±0.1 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -3.9
• 重原子数：
  31
• 可旋转键数：
  8
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  290
• 氢给体数：
  7
• 氢受体数：
  15

反应信息

• 作为产物：
  描述：

  3'-O-acetyl-N2-isobutyryl-2'-deoxyguanosine 在 吡啶 、 氨 、 tris(tetra-n-butylammonium) hydrogen pyrophosphate 、 sulfur 、 1,8-二氮杂双环[5.4.0]十一碳-7-烯 作用下， 以 乙腈 为溶剂， 反应 24.0h， 生成 dGTP(α-S)
  参考文献：
  名称：

  Synthesis of Nucleoside α-Thiotriphosphates via an Oxathiaphospholane Approach^†

  摘要：

  Nucleoside 5 '-O-(alpha-thiotriphosphates) were obtained in reactions of the appropriate nucleoside 5 '-O-(2-thio-1,3,2-oxathiaphospholanes) with pyrophosphate in the presence of DBU. The presented method allows also for preparation of alpha-seleno congeners and corresponding a-modified diphosphates.

  DOI：

  10.1021/ol050617r

文献信息

• Nucleic acid sequencing methods and systems
  申请人：Omniome, Inc.

  公开号：US10077470B2

  公开（公告）日：2018-09-18

  The present disclosure provides compositions, methods and systems for sequencing a template nucleic acid using a polymerase based, nucleic acid binding reaction involving examination of the interaction between a polymerase and template nucleic acid in the presence of one or more unlabeled nucleotides. The methods rely, in part, on identifying a base of a template nucleic acid during nucleic acid synthesis by controlling the sequencing reaction conditions. Template nucleic acid bases may be identified during an examination step followed by an optional incorporation step.

  本公开提供了使用基于聚合酶的核酸结合反应对模板核酸进行测序的组合物、方法和系统，该反应涉及在存在一种或多种未标记核苷酸的情况下检查聚合酶与模板核酸之间的相互作用。这些方法部分依赖于在核酸合成过程中通过控制测序反应条件来识别模板核酸的碱基。模板核酸碱基可在检查步骤中识别，然后进行可选的结合步骤。
• Method and system for sequencing nucleic acids
  申请人：OMNIOME, INC.

  公开号：US10246744B2

  公开（公告）日：2019-04-02

  Provided are compositions, methods and systems for determining the sequence of a template nucleic acid using a polymerase-based, sequencing-by-binding procedure. An examination step involves monitoring the interaction between a polymerase and template nucleic acid in the presence of one or more nucleotides. Identity of the next correct nucleotide in the sequence is determined without incorporation of any nucleotide into the structure of the primer by formation of a phosphodiester bond. An optional incorporation step can be used after the examination step to extend the primer by one or more nucleotides, thereby incrementing the template nucleotides that can be examined in a subsequent examination step. The sequencing-by-binding procedure does not require the use of labeled nucleotides or polymerases, but optionally can employ these reagents.

  本文提供了利用基于聚合酶的结合测序程序确定模板核酸序列的组合物、方法和系统。检查步骤包括在存在一个或多个核苷酸的情况下监测聚合酶与模板核酸之间的相互作用。在序列中确定下一个正确核苷酸的身份时，无需通过形成磷酸二酯键将任何核苷酸掺入引物结构中。在检查步骤之后，可以使用一个可选的结合步骤，将引物延长一个或多个核苷酸，从而增加可在后续检查步骤中检查的模板核苷酸。结合测序程序不需要使用标记核苷酸或聚合酶，但也可选择使用这些试剂。
• Isolation of target nucleic acids
  申请人：GENETICS RESEARCH, LLC

  公开号：US11142788B2

  公开（公告）日：2021-10-12

  The invention provides methods of isolating a target nucleic acid in a sample. A primer is hybridized to the target. A polymerase and modified nucleotide resistant to nuclease degradation are used to extend the primer to create a modified polynucleotide. The sample is exposed to a nuclease, thereby isolating the modified polynucleotide. Optionally, the target nucleic acid may be further protected by binding a protein in a sequence specific manner to one end of the target nucleic acid to create a protected target nucleic acid resistant to nuclease degradation. Thus, after exposing the sample to a nuclease, the modified polynucleotide and protected target nucleic acid are isolated.

  本发明提供了在样品中分离目标核酸的方法。引物与目标核酸杂交。使用聚合酶和耐核酸酶降解的修饰核苷酸扩展引物，形成修饰多核苷酸。将样本暴露于核酸酶，从而分离出修饰的多核苷酸。可以选择将蛋白质以序列特异的方式与目标核酸的一端结合，进一步保护目标核酸，以产生抗核酸酶降解的受保护目标核酸。这样，将样品暴露于核酸酶后，就能分离出修饰的多核苷酸和受保护的靶核酸。
• Systems and methods for data storage in nucleic acids
  申请人：Bishop Bryan

  公开号：US11339423B2

  公开（公告）日：2022-05-24

  Provided are methods and systems for encoding data into nucleic acid molecules. Methods and systems disclosed can include the use of promiscuous template nucleic acid molecules which enables data encoding using environmental modifications to yield encoded nucleic acid molecules.

  本文提供了将数据编码到核酸分子中的方法和系统。所公开的方法和系统可包括使用杂乱的模板核酸分子，从而利用环境修饰进行数据编码，产生编码的核酸分子。
• Exponential nucleic acid amplification using nicking endonucleases
  申请人：Keck Graduate Institute

  公开号：US20030082590A1

  公开（公告）日：2003-05-01

  The present invention provides methods and composition for exponential nucleic acid amplification using nicking agents. The invention is useful in many areas such as disease diagnosis, genetic variation detection and pre-mRNA alternative splicing analysis.

  本发明提供了使用裂解剂进行指数核酸扩增的方法和组合物。本发明可用于疾病诊断、基因变异检测和前 mRNA 替代剪接分析等多个领域。