galactose dialdehyde | 65207-56-9

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: galactose dialdehyde
• 英文别名: galactohexodialdose；D-galacto-hexodialdose；(2S,3R,4S,5R)-2,3,4,5-tetrahydroxyhexanedial
• CAS: 65207-56-9
• 化学式: C₆H₁₀O₆
• 分子量: 178.142
• InChiKey: VYPPEYAOCURAAE-GUCUJZIJSA-N

物化性质

• 沸点：
  543.3±50.0 °C(Predicted)
• 密度：
  1.566±0.06 g/cm3(Predicted)

计算性质

• 辛醇/水分配系数(LogP)：
  -3.2
• 重原子数：
  12
• 可旋转键数：
  5
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.67
• 拓扑面积：
  115
• 氢给体数：
  4
• 氢受体数：
  6

反应信息

• 作为反应物：
  描述：

  galactose dialdehyde 以 水 为溶剂， 以62%的产率得到(1S,3S,4R,5R,8R)-2,6-dioxabicyclo[3.2.1]octane-1,3,4,8-tetrol
  参考文献：
  名称：

  Galactose dialdehyde: the forgotten candidate for a protein cross-linker?

  摘要：

  Oxidation of D-galactose mediated by D-galactose oxidase [EC 1.1.3.9] gave a dialdehyde that occurs as two bicyclic structures in aqueous solution as established by C-13 labeling and NMR spectroscopy. The dialdehyde is very reactive at basic pH leading to complex reaction mixtures. A comparison was made with glutaraldehyde to assess its potential as protein cross-linker. (C) 2001 Published by Elsevier Science Ltd.

  DOI：

  10.1016/s0008-6215(01)00166-5
• 作为产物：
  描述：

  D-吡喃葡萄糖 在 galactose oxidase 、 氧气 作用下， 生成 galactose dialdehyde
  参考文献：
  名称：

  Preparation and Utilization of a Biosensor Based on Galactose Oxidase

  摘要：

  一种用于D-半乳糖测定的酶电极，通过将固定有固定化半乳糖氧化酶（E.C. 1.1.3.9）或共固定半乳糖氧化酶和过氧化氢酶（E.C. 1.11.1.6）的载体固定到Clark型氧传感器上制备而成。酶可以通过使用环己基异氰酸酯和戊二醛的Ugi反应在部分水解的尼龙网格或天然胶原膜上固定。生物传感器通过固定化半乳糖氧化酶的特异性活性、表观米氏常数K_M(app.)和稳定性（通过时间和执行分析的数量来表达）进行表征。还测试了生物传感器的底物特异性和反应混合物的pH和温度对响应幅度的影响。制备的生物传感器被用于测定疑似半乳糖尿病患者的血浆和血清中的D-半乳糖含量。

  DOI：

  10.1135/cccc19922287

文献信息

• [EN] USE OF FUCOSYLATION INHIBITOR FOR PRODUCING AFUCOSYLATED ANTIBODY<br/>[FR] UTILISATION D'UN INHIBITEUR DE FUCOSYLATION POUR PRODUIRE UN ANTICORPS AFUCOSYLÉ
  申请人：BRISTOL MYERS SQUIBB CO

  公开号：WO2021127414A1

  公开（公告）日：2021-06-24

  The present invention provides inhibitors of fucosylation during protein expression from mammalian cells. The inhibitors are derived from rhamnose and act by inhibition of GDP-mannose 4,6-dehydratase (GMD). The invention further provides methods of making proteins with reduced level of fucosylation, such as antibodies and antibodies made by the methods of the present invention. Such hypofucosylated or nonfucosylated antibodies may find use, for example, in treatment of human disease in which is it therapeutically beneficial to direct antibody dependent cellular cytotoxicity (ADCC) mediated killing of cells expressing the antibody target on their surface, for example in depletion of Tregs in cancer patients using a hypofucosylated or nonfucosylated anti-CTLA-4 antibody.

  本发明提供了在哺乳动物细胞中蛋白质表达过程中抑制富糖基化的抑制剂。这些抑制剂源自鼠李糖，并通过抑制 GDP-甘露糖4,6-脱水酶（GMD）来发挥作用。该发明还提供了制备具有降低富糖基化水平的蛋白质的方法，例如通过本发明的方法制备的抗体和抗体。这种低富糖化或无富糖化的抗体可以在治疗人类疾病中发挥作用，例如在治疗中对表达抗体靶点的细胞进行抗体依赖性细胞毒作用（ADCC）介导的杀伤，例如在癌症患者中使用低富糖化或无富糖化的抗CTLA-4抗体去除Treg细胞。
• CROSS-LINKED COMPOSITIONS
  申请人：Preiss-Bloom Orahn

  公开号：US20110110882A1

  公开（公告）日：2011-05-12

  Improved compositions comprising a cross-linkable protein or polypeptide, and a non-toxic material which induces cross-linking of the cross-linkable protein. The compositions are optionally and preferably prepared in a non-phosphate buffer solvent. Optionally and preferably, the cross-linkable protein includes gelatin and any gelatin variant or variant protein as described herein. Optionally and preferably, the non-toxic material comprises transglutaminase (TG), which may optionally comprise any type of calcium dependent or independent transglutaminase, which may for example optionally be a microbial transglutaminase (mTG).

  改进的组合物包括可交联的蛋白质或多肽，以及诱导可交联蛋白质交联的非毒性材料。该组合物可以选择性地和最好地在非磷酸盐缓冲溶剂中制备。可选地和最好地，可交联蛋白质包括明胶和本说明书中所述的任何明胶变体或变体蛋白质。可选地和最好地，非毒性材料包括谷氨酰胺转移酶（TG），该谷氨酰胺转移酶可以选择包括任何类型的钙依赖性或独立性谷氨酰胺转移酶，例如可以是微生物谷氨酰胺转移酶（mTG）。
• Novel electrochemical sensing of galactose using GalOxNPs/CHIT modified pencil graphite electrode
  作者：Mamta Sharma、Pooja Yadav、Minakshi Sharma

  DOI：10.1016/j.carres.2019.107749

  日期：2019.9

  For the construction of galactose biosensor, chitosan was electropolymerised onto the pencil graphite electrode. This chitosan modified pencil graphite electrode acts as good matrix for immobilization of enzyme nanoparticles of galactose oxidase. Development of this nanocomposite was further confirmed by Fourier transform infrared spectroscopy and scanning electron microscopy. The presence of chitosan

  为了构建半乳糖生物传感器，将壳聚糖电聚合到笔形石墨电极上。这种壳聚糖修饰的铅笔状石墨电极可作为固定半乳糖氧化酶酶纳米颗粒的良好基质。通过傅立叶变换红外光谱和扫描电子显微镜进一步证实了该纳米复合材料的发展。壳聚糖的存在使得本发明的半乳糖生物传感器更加有效，可再现和稳定。灵敏度为7×10-3 mA / mM / cm2，线性范围为0.05至25 mM，更好的检出限为0.05 mM。当半乳糖溶液中加入0.5 mM和1 mM的样品时，分析回收率分别为98.6％和97.6％。与早期报道的生物传感器相比，具有更好的存储稳定性（90天）。
• Di-enzymatic dentifrice
  申请人：Laclede Professional Products, Inc.

  公开号：US04578265A1

  公开（公告）日：1986-03-25

  A di-enzymatic dentifrice is provided which contains an oxidizable substrate and an oxidoreductase enzyme specific to such substrate for producing hydrogen peroxide upon oral application of the dentifrice and further contains a thiocyanate salt and lactoperoxidase for interacting with hydrogen peroxide to produce a hypothiocyanate bacterial inhibitor. An illustrative enzymatic system for this purpose contains glucose, glucose oxidase, potassium thiocyanate and lactoperoxidase.

  提供了一种双酶牙膏，其中包含可氧化的底物和特异于该底物的氧化还原酶酶，用于在口腔应用牙膏时产生过氧化氢，并进一步包含硫氰酸盐和乳过氧化物酶，以与过氧化氢相互作用产生一种假硫氰酸盐细菌抑制剂。这种用途的示例酶系统包括葡萄糖、葡萄糖氧化酶、硫氰酸钾和乳过氧化物酶。
• Nucleic acids useful for enzymatic conversion of GDP-mannose to GDP-fucose
  申请人：Cytel Corporation

  公开号：US20030134403A1

  公开（公告）日：2003-07-17

  This invention provides methods for practical enzymatic conversion of GDP-mannose to GDP-fucose. These methods are useful for efficient synthesis of reactants used in the synthesis of fucosylated oligosaccharides.

  本发明提供了一种实用的酶促转化GDP-甘露糖为GDP-岩藻糖的方法。这些方法对于高效合成富含岩藻糖基的寡糖反应物非常有用。