6-(3,4-Dihydroxyphenyl)-2,4-dioxohex-5-enoic acid | 89296-49-1

• 分子结构分类: 有机化合物 - 苯丙烷和聚酮 - 肉桂酸及其衍生物
• 英文名称: 6-(3,4-Dihydroxyphenyl)-2,4-dioxohex-5-enoic acid
• 英文别名: 6-(3,4-dihydroxyphenyl)-2,4-dioxohex-5-enoic acid
• CAS: 89296-49-1
• 化学式: C₁₂H₁₀O₆
• 分子量: 250.2
• InChiKey: VDADJCUBZJQTKA-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  1.4
• 重原子数：
  18
• 可旋转键数：
  5
• 环数：
  1.0
• sp3杂化的碳原子比例：
  0.08
• 拓扑面积：
  112
• 氢给体数：
  3
• 氢受体数：
  6

文献信息

• ENZYMES OF LUCIFERIN BIOSYNTHESIS AND USE THEREOF
  申请人：Light Bio, Inc

  公开号：EP3816282A1

  公开（公告）日：2021-05-05

  Present invention is aimed at identification of new fungal luciferin biosynthesis enzymes, nucleic acids able to encode these enzymes, and proteins able to catalyze certain stages of the fungal luciferin biosynthesis. The invention also provides for application of nucleic acids for producing said enzymes in a cell or organism. Methods for in vitro or in vivo preparation of chemical compounds identical to fungal luciferins and preluciferins are also provided. Vectors comprising nucleic acid described in the present invention are also provided. In addition, the present invention provides expression cassettes comprising the nucleic acid of the present invention and regulatory elements necessary for nucleic acid expression in a selected host cell. Besides, cells, stable cell lines, transgenic organisms (e.g. plants, animals, fungi, or microorganisms) including nucleic acids, vectors, or expression cassettes of the present invention are also provided. Present invention also provides combinations of nucleic acids to obtain autonomously luminous cells, cell lines, or transgenic organisms. In preferred embodiments, cells or transgenic organisms are capable to produce fungal luciferin from precursors. In some embodiments, cells or transgenic organisms are capable to produce fungal preluciferin from precursors. In some embodiments, cells or transgenic organisms are capable of bioluminescence in the presence of a fungal luciferin precursor. In some embodiments, cells or transgenic organisms are capable of autonomous bioluminescence. Combinations of proteins for producing luciferin or its precursors from more simple chemical compounds are also provided. A kit containing nucleic acids, vectors, or expression cassettes of the present invention for producing luminous cells, cell lines, or transgenic organisms is also provided.

  本发明旨在鉴定新的真菌荧光素生物合成酶、能够编码这些酶的核酸以及能够催化真菌荧光素生物合成某些阶段的蛋白质。本发明还提供了核酸在细胞或生物体内产生上述酶的应用。本发明还提供了体外或体内制备与真菌荧光素和预荧光素相同的化合物的方法。还提供了包含本发明所述核酸的载体。此外，本发明还提供了包含本发明核酸和在所选宿主细胞中表达核酸所需的调控元件的表达盒。此外，本发明还提供了包括本发明核酸、载体或表达盒的细胞、稳定细胞系、转基因生物（如植物、动物、真菌或微生物）。本发明还提供了核酸组合，以获得自主发光的细胞、细胞系或转基因生物。在优选的实施方案中，细胞或转基因生物能够从前体产生真菌荧光素。在某些实施方案中，细胞或转基因生物能够从前体产生真菌前荧光素。在某些实施方案中，细胞或转基因生物能够在真菌荧光素前体存在的情况下发出生物荧光。在某些实施方案中，细胞或转基因生物能够自主发出生物荧光。此外，还提供了从更简单的化合物中生产荧光素或其前体的蛋白质组合。还提供了含有本发明核酸、载体或表达盒的试剂盒，用于生产发光细胞、细胞系或转基因生物。
• [EN] METHOD FOR PRODUCING BIOLUMINESCENT PLANTS<br/>[FR] MÉTHODE DE FABRICATION DE PLANTES BIOLUMINESCENTES
  申请人：WOODLIGHT

  公开号：WO2021140108A1

  公开（公告）日：2021-07-15

  La présente invention se rapporte à une méthode de fabrication de plantes bioluminescentes par introduction des gènes de la luciférase et ceux de la biosynthèse de luciférine dans les chloroplastes de plantes.