TETRAPEPTIDIC TRYPTASE SUBSTRATES AND ASSAY FOR TRYPTASE ACTIVITY USING SAME
申请人:PROMEGA CORPORATION
公开号:EP1358205A2
公开(公告)日:2003-11-05
NOVEL FERULATE ESTERASE ISOLATED FROM LACTOBACCILLUS FERMENTUM
申请人:The United States of America, as represented by the Secretary of Agriculture
公开号:US20160244734A1
公开(公告)日:2016-08-25
The cDNA and amino acid sequences of a ferulate esterase obtained from
Lactobacillus fermentum
NRRL B-1932 is determined. An expression vector for expression of the ferulate esterase gene is generated. The recombinate ferulate esterase gene and transcribed protein contains a linker sequence and 6× HIS tag for purification. Enzymatic activity of the recombinant protein is determined.
US9416354B1
申请人:——
公开号:US9416354B1
公开(公告)日:2016-08-16
[EN] TRYPTASE SUBSTRATES AND ASSAY FOR TRYPTASE ACTIVITY USING SAME<br/>[FR] SUBSTRATS DE TRYPTASES ET DOSAGE DESTINE A MESURER L'ACTIVITE DES TRYPTASE AU MOYEN DESDITS SUBSTRATS
申请人:PROMEGA CORP
公开号:WO2002034765A2
公开(公告)日:2002-05-02
The invention is directed to synthetic polypeptide substrates for tryptase enzymes and assays for tryptase activity that utilize the synthetic polypeptide substrates. The preferred synthetic polypeptide substrates are tetramers of the formula P4-P3-P2-P1, wherein the substrate is selected from the group consisting of P-R-N-K (SEQ.ID.NO:2), P-K-N-K (SEQ.ID.NO:3), P-R-N-R (SEQ.ID.NO:4), P-K-N-R (SEQ.ID.NO:5), P-A-N-K (SEQ.ID.NO:6), and P-R-T-K (SEQ.ID:NO:7).