4,4′-[（8,16-二氢-8,16-二氧二苯并[a，j]苝-2,10-二基）二氧基]二丁酸 | 243670-14-6

• 分子结构分类: 有机化合物 - 苯类化合物 - 菲及其衍生物
• 中文名称: 4,4′-[（8,16-二氢-8,16-二氧二苯并[a，j]苝-2,10-二基）二氧基]二丁酸
• 英文名称: 4,4'-[2,10-(8,16-dihydro-8,16-dioxodibenzo[a,j]perylene)bis(oxy)]bisbutanoic acid
• 英文别名: 4,4'-[(8,16-Dihydro-8,16-dioxodibenzo[a,j]perylene-2,10-diyl)dioxy]dibutyric acid；4-[[19-(3-carboxypropoxy)-9,22-dioxo-6-heptacyclo[13.11.1.12,10.03,8.016,21.023,27.014,28]octacosa-1,3(8),4,6,10,12,14(28),15(27),16(21),17,19,23,25-tridecaenyl]oxy]butanoic acid
• CAS: 243670-14-6
• 化学式: C₃₆H₂₆O₈
• 分子量: 586.598
• InChiKey: XZWIRUDAKQRKFW-UHFFFAOYSA-N

物化性质

• 熔点：
  231-235 °C

计算性质

• 辛醇/水分配系数(LogP)：
  6.3
• 重原子数：
  44
• 可旋转键数：
  10
• 环数：
  7.0
• sp3杂化的碳原子比例：
  0.17
• 拓扑面积：
  127
• 氢给体数：
  2
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  N-羟基丁二酰亚胺 、 4,4′-[（8,16-二氢-8,16-二氧二苯并[a，j]苝-2,10-二基）二氧基]二丁酸 在 N,N'-二环己基碳二亚胺 作用下， 以 N,N-二甲基甲酰胺 为溶剂， 反应 20.0h， 以75%的产率得到4,4′-[（8,16-二氢-8,16-二氧二苯并[a，j]苝-2,10-二基）二氧基]二丁酸二（N-琥珀酰亚胺酯）
  参考文献：
  名称：

  Far-Red Dibenzo[a,j]Perylene-8,16-Dione Dyes as Fluorescent Enzyme Substrates and Tags for Biomolecules

  摘要：

  The synthesis of fluorescent 2,10-derivatives of the title chromophore are described. The substituents contain amino-reactive N-hydroxysuccinimde eater and thiol-reactive iodoacetamide groups for attachment to biomolecules. A phosphate derivative was also prepared as an enzyme substrate.

  DOI：

  10.1080/00397910008087092
• 作为产物：
  描述：

  dimethyl 4,4'-[2,10-(8,16-dihydro-8,16-dioxodibenzo[a,j]perylene)bis(oxy)]bisbutanoate 在 sodium hydroxide 作用下， 以90%的产率得到4,4′-[（8,16-二氢-8,16-二氧二苯并[a，j]苝-2,10-二基）二氧基]二丁酸
  参考文献：
  名称：

  Far-Red Dibenzo[a,j]Perylene-8,16-Dione Dyes as Fluorescent Enzyme Substrates and Tags for Biomolecules

  摘要：

  The synthesis of fluorescent 2,10-derivatives of the title chromophore are described. The substituents contain amino-reactive N-hydroxysuccinimde eater and thiol-reactive iodoacetamide groups for attachment to biomolecules. A phosphate derivative was also prepared as an enzyme substrate.

  DOI：

  10.1080/00397910008087092

文献信息

• Biological substance detection method
  申请人：Konica Minolta, Inc.

  公开号：EP2757378A2

  公开（公告）日：2014-07-23

  The present invention provides a biological substance detection method for specifically detecting a biological substance from a pathological specimen, by which method, when immunostaining using a fluorescent label and staining for morphological observation using a staining agent for morphological observation are simultaneously performed, the results of fluorescence observation and immunostaining can be assessed properly even if the fluorescent label and/or the staining agent is/are deteriorated by irradiation with an excitation light. The biological substance detection method according to the present invention is characterized in that the brightness retention rate of an immunostained part is in a range of 80% to 120% in relation to the brightness retention rate of a part stained for morphological observation when the fluorescent label used for the immunostaining is observed.

  本发明提供了一种从病理标本中特异性检测生物物质的生物物质检测方法，通过该方法，当使用荧光标签进行免疫染色和使用形态观察用染色剂进行形态观察染色同时进行时，即使荧光标签和/或染色剂因激发光照射而变质，也能正确评估荧光观察和免疫染色的结果。根据本发明的生物物质检测方法的特点是，在观察用于免疫染色的荧光标签时，免疫染色部分的亮度保持率与用于形态观察的染色部分的亮度保持率相比在 80% 至 120% 的范围内。
• METHOD FOR DETECTING BIOLOGICAL MATERIAL
  申请人：Konica Minolta, Inc.

  公开号：EP2833140A1

  公开（公告）日：2015-02-04

  The present invention provides a staining method in which the fluorescent staining properties in a fluorescently-immunostained specimen are not reduced even when an oil-based mounting medium is used. The present invention also provides a method of preventing deterioration of a fluorescent label caused by irradiation with excitation light and improving the light resistance in a fluorescently-immunostained specimen obtained by the staining method. The biological substance detection method according to the present invention is a biological substance detection method for specifically detecting a biological substance from a pathological specimen, which comprises the steps of: immunostaining the specimen with a fluorescent label; immobilizing the thus stained specimen; and mounting the thus immobilized specimen using a mounting medium comprising an organic solvent not freely miscible with water. In the biological substance detection method, the above-described mounting medium further comprises a discoloration inhibitor.

  本发明提供了一种染色方法，在这种方法中，即使使用油基装载介质，也不会降低荧光免疫染色标本的荧光染色特性。本发明还提供了一种方法，可防止荧光标签因激发光照射而变质，并提高通过染色方法获得的荧光免疫染色标本的耐光性。根据本发明的生物物质检测方法是一种从病理标本中特异性检测生物物质的生物物质检测方法，它包括以下步骤：用荧光标签对标本进行免疫染色；将这样染色的标本固定；以及使用由与水不能自由混溶的有机溶剂组成的装载介质装载这样固定的标本。在生物物质检测方法中，上述安装介质还包括变色抑制剂。
• STAINING AGENT FOR STAINING TISSUE, PRODUCTION METHOD FOR STAINING AGENT FOR STAINING TISSUE, AND TISSUE STAINING KIT INCLUDING STAINING AGENT FOR STAINING TISSUE
  申请人：Konica Minolta, Inc.

  公开号：EP2966445A1

  公开（公告）日：2016-01-13

  An object of the present invention is to provide: a staining agent for tissue staining which has an improved fluorescence signal evaluation accuracy; and a tissue staining kit comprising the staining agent. The staining agent for tissue staining contains, as a staining component, dye-resin particles comprising thermosetting resin particles and a fluorescent dye immobilized on the resin particles, wherein the resin particles contains a substituent having an electric charge opposite to that of the fluorescent dye and forms an ionic bond or a covalent bond with the fluorescent dye, and the dye-resin particles have a particle size variation coefficient of 15% or less.

  本发明的目的是提供：一种用于组织染色的染色剂，它具有更高的荧光信号评估精度；以及一种包含该染色剂的组织染色试剂盒。用于组织染色的染色剂含有作为染色成分的染料-树脂颗粒，染料-树脂颗粒包括热固性树脂颗粒和固定在树脂颗粒上的荧光染料，其中树脂颗粒含有与荧光染料电荷相反的取代基，并与荧光染料形成离子键或共价键，染料-树脂颗粒的粒度变化系数为 15%或更小。
• RESIN PARTICLES FOR FLUORESCENT LABELS
  申请人：Konica Minolta, Inc.

  公开号：EP2966435A1

  公开（公告）日：2016-01-13

  An object of the present invention is to provide a fluorophore having excellent initial emission intensity and light resistance, which is not easily discolored and does not affect the evaluation of the number of bright spots even when it is slightly discolored. The resin particle for fluorescence labeling is characterized by comprising a fluorescent dye immobilized in a resin particle, the fluorescent dye satisfying both of the following conditions (1) and (2): (1) the fluorescent dye alone in an aqueous solution has a concentration-dependent peak emission intensity in a range of 30 to 80 µM; and (2) the emission intensity at a concentration of 100 µM is not less than 80% of the peak emission intensity. It is preferred that the Stoke's shift of the fluorescent dye in an aqueous solution is not less than 25 nm. The fluorescent dye may be subjected to a solubilization treatment (such as an acid treatment). It is preferred that the fluorescent dye be encapsulated in a resin particle comprising a thermosetting resin.

  本发明的目的是提供一种荧光团，该荧光团具有优异的初始发射强度和耐光性，不易褪色，即使轻微褪色也不影响对亮点数量的评估。荧光标记用树脂颗粒的特点是由固定在树脂颗粒中的荧光染料组成，该荧光染料满足以下两个条件（1）和（2）：(1) 水溶液中的单独荧光染料的峰值发射强度随浓度变化，范围在 30 至 80 µM；以及 (2) 浓度为 100 µM 时的发射强度不小于峰值发射强度的 80%。荧光染料在水溶液中的斯托克偏移最好不小于 25 nm。可对荧光染料进行增溶处理（如酸处理）。荧光染料最好封装在由热固性树脂组成的树脂颗粒中。
• FLUORESCENT NANOPARTICLES FOR BIOMOLECULAR STAINING AND MANUFACTURING METHOD FOR SAME
  申请人：Konica Minolta, Inc.

  公开号：EP3012632A1

  公开（公告）日：2016-04-27

  For fluorescent nanoparticles having a zeta potential of -10 mV to -60 mV at pH 7.0 or a zeta potential of 0 mV to -10 mV in a buffer of pH 6.0 to 8.0, an appropriate electrical repulsive force can be generated between biomolecules that are generally negatively charged and the fluorescent nanoparticles. As a result, non-specific binding between the fluorescent nanoparticles and the biomolecules is surppressed and the fluorescent nanoparticles are specifically bound to a biomolecule to be stained through interaction stronger than the electrical repulsive force, so that the visibility of the specific biomolecule to be stained can be improved. Further, since an appropriate electrical repulsive force is also generated between the fluorescent nanoparticles themselves, aggregation of the fluorescent nanoparticles can be inhibited and the dispersibility in a staining solution can thereby be maintained.

  对于在 pH 值为 7.0 时 zeta 电位为 -10 mV 至 -60 mV 或在 pH 值为 6.0 至 8.0 的缓冲液中 zeta 电位为 0 mV 至 -10 mV 的荧光纳米粒子，可在通常带负电的生物大分子与荧光纳米粒子之间产生适当的电排斥力。因此，荧光纳米粒子与生物大分子之间的非特异性结合被抑制，荧光纳米粒子通过强于电排斥力的相互作用与待染色的生物大分子特异性结合，从而提高了待染色的特定生物大分子的可见度。此外，由于荧光纳米粒子本身之间也会产生适当的电排斥力，因此可以抑制荧光纳米粒子的聚集，从而保持其在染色溶液中的分散性。