| 144491-72-5

• 分子结构分类: 有机化合物 - 有机氧化合物
• CAS: 144491-72-5
• 化学式: C₃₄H₄₈N₄O₂₁S₂*2Na
• 分子量: 958.881
• InChiKey: GXUVZZNHOOEBQZ-YMPOQWKNSA-L

计算性质

• 辛醇/水分配系数(LogP)：
  -9.42
• 重原子数：
  62.0
• 可旋转键数：
  18.0
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.62
• 拓扑面积：
  412.49
• 氢给体数：
  12.0
• 氢受体数：
  22.0

反应信息

• 作为产物：
  描述：

  chitotriose 、 2-naphthylamino-6,8-disulfonic acid monopotassium salt 在 sodium hydroxide 、 sodium cyanoborohydride 作用下， 生成
  参考文献：
  名称：

  A new method for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates

  摘要：

  A new method is described for sequencing linear oligosaccharides on gels using charged, fluorescent conjugates. The reducing ends of various mono-, di-, tri-, and tetra-saccharides were conjugated with monopotassium 7-amino-1,3-naphthalenedisulfonate (a fluorescent and negatively charged compound) by reductive amination using sodium cyanoborohydride. The sugar conjugates were purified by preparative gradient polyacrylamide gel electrophoresis followed by a newly developed technique involving their semi-dry transfer to positively charged nylon membranes and elution with sodium chloride. The structures of a monosaccharide- and trisaccharide-conjugate were established by f.a.b.-m.s. and 2D n.m.r. Seven linear oligosaccharide-fluorescent conjugates were treated sequentially with exoglycosidases and with endoglycosidases. Analysis of the products by gel electrophoresis provided sequence information. These methods may be useful for sequencing oligosaccharides that are chemically or enzymically (endoglycosidase) released from glycoproteins, glycolipids, and proteoglycans.

  DOI：

  10.1016/s0008-6215(00)90538-x