4,7-二(3-氯-2-磺基苯基)-1,10-菲咯啉-2,9-二甲酸 | 112076-76-3

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 中文名称: 4,7-二(3-氯-2-磺基苯基)-1,10-菲咯啉-2,9-二甲酸
• 英文名称: 4,7-Bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid
• 英文别名: 4,7-bis(3-chloro-2-sulfophenyl)-1,10-phenanthroline-2,9-dicarboxylic acid
• CAS: 112076-76-3
• 化学式: C₂₆H₁₄Cl₂N₂O₁₀S₂
• 分子量: 649.4
• InChiKey: VEJHUGGPLQWGPR-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.2
• 重原子数：
  42
• 可旋转键数：
  6
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  226
• 氢给体数：
  4
• 氢受体数：
  12

文献信息

• Dual resonance energy transfer nucleic acid probes
  申请人：——

  公开号：US20030129611A1

  公开（公告）日：2003-07-10

  Dual nucleic acid probes with resonance energy transfer moieties are provided. In particular, fluorescent or luminescent resonance energy transfer moieties are provided on hairpin stem-loop molecular beacon probes that hybridize sufficiently near each other on a subject nucleic acid, e.g. mRNA, to generate an observable interaction. The invention also provides lanthanide chelate luminescent resonance energy transfer moieties on linear and stem-loop probes that hybridize sufficiently near each other on a subject nucleic acid to generate an observable interaction. The invention thereby provides detectable signals for rapid, specific and sensitive hybridization determination in vivo. The probes are used in methods of detection of nucleic acid target hybridization for the identification and quantification of tissue and cell-specific gene expression levels, including response to external stimuli, such as drug candidates, and genetic variations associated with disease, such as cancer.

  提供带有谐振能量转移基团的双核酸探针。具体来说，在发明中，在发生足够接近的发夹式干环分子信标探针上提供了荧光或发光谐振能量转移基团，这些探针在主体核酸（如mRNA）上发生足够接近的杂交以产生可观察的相互作用。该发明还提供了在线性和发夹式探针上的镧系螯合物发光谐振能量转移基团，这些探针在主体核酸上发生足够接近的杂交以产生可观察的相互作用。因此，该发明提供了用于体内快速、特异和敏感的杂交测定的可检测信号。这些探针用于检测核酸靶标的杂交的方法，以确定和量化组织和细胞特异基因表达水平，包括对外部刺激（如药物候选物）的反应，以及与疾病（如癌症）相关的遗传变异。
• Activatable probes and methods for in vivo gene detection
  申请人：Bao Gang

  公开号：US20050287548A1

  公开（公告）日：2005-12-29

  Probes for detecting a target polynucleotide are provided. One aspect provides a molecular beacon probe set wherein the donor molecular beacon comprises a quantum dot and an acceptor molecular beacon comprises at least one reporter. The probes optionally comprise a protein transduction domain, targeting signal, or a combination thereof. Methods for detecting target polynucleotides using the disclosed probes are also provided.

  提供用于检测靶向多核苷酸的探针。一个方面提供了一种分子信标探针集合，其中供体分子信标包括量子点，受体分子信标包括至少一个报告基团。这些探针可以选择性地包括蛋白质转导域、靶向信号或二者的组合。还提供了使用所述探针检测靶向多核苷酸的方法。
• Methods for the preparation of chemically misaminoacylated tRNA via protective groups
  申请人：AMBERGEN, INC.

  公开号：US20030219780A1

  公开（公告）日：2003-11-27

  The present invention relates to methods for the preparation of chemically aminoacylated tRNAs for the purpose of introduction of markers into nascent proteins. The present invention also relates to methods for the non-radioactive labeling, detection, quantitation and isolation of nascent proteins translated in a cellular or cell-free translation system utilizing chemically aminoacylated tRNAs. tRNA molecules are misaminoacylated with non-radioactive markers which may be non-native amino acids, amino acid analogs or derivatives. Markers may comprise cleavable moieties, detectable labels, reporter properties wherein markers incorporated into protein can be distinguished from unincorporated markers, or coupling agents which facilitate the detection and isolation of nascent protein from other components of the translation system.

  本发明涉及用于制备化学氨酰化tRNA的方法，目的是将标记物引入新生蛋白质中。本发明还涉及用于在细胞或无细胞翻译系统中翻译的新生蛋白质的非放射性标记、检测、定量和分离的方法，利用化学氨酰化的tRNA。tRNA分子被非放射性标记物误氨酰化，这些标记物可以是非天然氨基酸、氨基酸类似物或衍生物。标记物可能包括可切割的基团、可检测的标签、报告性质，其中蛋白质中包含的标记物可以与未包含的标记物区分开来，或者促进检测和从翻译系统的其他组分中分离新生蛋白质的耦合剂。
• Immunoassay methods and reagents and methods for producing the latter
  申请人：CYBERFLUOR INC.

  公开号：EP0290269A2

  公开（公告）日：1988-11-09

  Immunoassay and methods for using fluorescent chelates of lanthanide metal ions in conjunction with immunoreactive bodies to permit fluorescent assay in liquid or dry samples. The assay reagent comprises a residue of an immunoreactive body linked to a residue of a protein or polypeptide. The protein or polypeptide is labelled by substitution with a ligand forming a fluorescent chelate with lanthanide metal ion such as europium. The reagent when used in an immunoassay, binds to the immobilized immunoreactive body and excess is washed away. The sample can be dried for later analysis by a suitable fluoremeter.

  将镧系金属离子的荧光螯合物与免疫活性体结合使用以允许在液体或干燥样品中进行荧光测定的免疫测定和方法。 检测试剂包括与蛋白质或多肽残基相连的免疫活性体残基。 蛋白质或多肽通过与镧系金属离子（如铕）形成荧光螯合物的配体取代而被标记。 用于免疫测定时，试剂与固定的免疫反应体结合，多余的试剂被洗去。 样品可以烘干，以便以后用合适的荧光测定仪进行分析。
• Multiple fluorescence labelling with europium chelators
  申请人：CYBERFLUOR INC.

  公开号：EP0354847A2

  公开（公告）日：1990-02-14

  A conjugate for use in a labelling system comprises avidin or streptavidin linked to a carrier particle, having more than fifteen amino groups on its surface, which are individually capable of being labelled with an operable label. The carrier particle is capable of being linked to the avidin or streptavidin to form the conjugate. The conjugate is particularly useful in forming a fluorescent reagent system. The carrier particle is labelled with a plurality of ligand moieties forming with a lanthanide metal ion, fluorescent chelates attached to the plurality of amino groups to provide at least fifteen labels. The fluorescent reagent system, when excited to fluoresce, emits fluorescent radiation with an intensity amplified compared to intensity of fluorescent radiation emitted by the plurality of fluorescent lanthanide metal ion chelate moieties in a solution whereby the fluorescent reagent is void of any quenching effects due to multiple labelling in the fluorescent reagent system. The fluorescent labelling system is particularly useful in binding assays.

  一种用于标记系统的共轭物由与载体颗粒相连的阿维丁或链霉亲和素组成，载体颗粒表面有 15 个以上的氨基基团，这些氨基基团可被单独标记为可操作的标签。载体颗粒可与阿维丁或链霉亲和素连接形成共轭物。这种共轭物特别适用于形成荧光试剂系统。载体颗粒被多个配体分子标记，这些配体分子与镧系金属离子、荧光螯合物连接到多个氨基上，以提供至少 15 个标记。当荧光试剂系统被激发发出荧光时，与溶液中多个荧光镧系金属离子螯合物分子发出的荧光辐射强度相比，荧光试剂系统发出的荧光辐射强度被放大，从而使荧光试剂没有任何因荧光试剂系统中多重标记而产生的淬灭效应。荧光标记系统尤其适用于结合试验。