The present invention provides a colorimetric method that can perform a simple and reliable analysis in a short time. The method includes transferring an electron from an analyte to a coloring reagent that produces color by reduction via a mediator by using an oxidoreductase; and performing qualitative or quantitative analysis of the analyte by measuring color produced in the coloring reagent. The enzyme reaction of this method is a single stage reaction, and the color production reaction occurs via the mediator. Therefore, the measurement can be performed in a short time. Since this reaction requires neither hydrogen peroxide nor oxygen, the measured values are highly reliable.
本发明提供了一种比色法,可在短时间内进行简单可靠的分析。该方法包括利用氧化还原酶将电子从被分析物转移到通过介质还原产生颜色的显色试剂中;以及通过测量显色试剂中产生的颜色对被分析物进行定性或定量分析。该方法中的酶反应是一个单级反应,而颜色生成反应是通过介质发生的。因此,测量可在短时间内完成。由于该反应既不需要
过氧化氢,也不需要
氧气,因此测量值非常可靠。