申请人:ARKRAY, Inc.
公开号:EP1515144A1
公开(公告)日:2005-03-16
A method for measuring an analyte in a sample by using a redox reaction is provided, which gives values with excellent reliability. Prior to the redox reaction, at least one of a sulfonic acid compound and a nitro compound is added to the sample to eliminate the influence of hemoglobin and any hemoglobin degradation products as reducing substances contained in the sample. Subsequently, a reducing or oxidizing substance derived from the analyte is caused to generate, and the amount thereof is measured by the redox reaction. The amount of the analyte is determined from the measurement value. The sulfonic acid compound may be sodium lauryl sulfate, and the nitro compound may be 4-nitrophenol, etc.
本发明提供了一种利用氧化还原反应测量样品中分析物的方法,其测量值非常可靠。在进行氧化还原反应之前,先将磺酸化合物和硝基化合物中的至少一种添加到样品中,以消除样品中含有的血红蛋白和任何血红蛋白降解产物作为还原性物质的影响。随后,使分析物产生还原性或氧化性物质,并通过氧化还原反应测量其含量。根据测量值确定被分析物的量。磺酸化合物可以是十二烷基硫酸钠,硝基化合物可以是 4-硝基苯酚等。