Acetic acid (2R,3S,4R,5R,6R)-3-acetoxy-2-acetoxymethyl-5-(4-nitro-benzyloxycarbonylamino)-6-((2R,3S,4S,5R,6S)-3,4,5-triacetoxy-6-octyloxy-tetrahydro-pyran-2-ylmethoxy)-tetrahydro-pyran-4-yl ester | 304466-83-9

• 分子结构分类: 有机化合物 - 脂质和类脂质分子 - 脂肪酰基
• 英文名称: Acetic acid (2R,3S,4R,5R,6R)-3-acetoxy-2-acetoxymethyl-5-(4-nitro-benzyloxycarbonylamino)-6-((2R,3S,4S,5R,6S)-3,4,5-triacetoxy-6-octyloxy-tetrahydro-pyran-2-ylmethoxy)-tetrahydro-pyran-4-yl ester
• CAS: 304466-83-9
• 化学式: C₄₀H₅₆N₂O₂₀
• 分子量: 884.886
• InChiKey: HKTRCURTPDIUPR-ZTFBNPIXSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  3.25
• 重原子数：
  62.0
• 可旋转键数：
  22.0
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.68
• 拓扑面积：
  276.19
• 氢给体数：
  1.0
• 氢受体数：
  20.0

反应信息

• 作为反应物：
  描述：

  Acetic acid (2R,3S,4R,5R,6R)-3-acetoxy-2-acetoxymethyl-5-(4-nitro-benzyloxycarbonylamino)-6-((2R,3S,4S,5R,6S)-3,4,5-triacetoxy-6-octyloxy-tetrahydro-pyran-2-ylmethoxy)-tetrahydro-pyran-4-yl ester 在 10percent Pd/C 氢气 、 sodium methylate 作用下， 以 甲醇 为溶剂， 反应 27.0h， 生成 N-[(2R,3R,4R,5S,6R)-4,5-Dihydroxy-6-hydroxymethyl-2-((2R,3R,4S,5R,6S)-3,4,5-trihydroxy-6-octyloxy-tetrahydro-pyran-2-ylmethoxy)-tetrahydro-pyran-3-yl]-acetamide
  参考文献：
  名称：

  Differential Carbohydrate Recognition of Two GlcNAc-6-sulfotransferases with Possible Roles in L-Selectin Ligand Biosynthesis

  摘要：

  Two human GlcNAc-6-sulfotransferases, CHST2 and HEC-GlcNAc6ST, have been recently identified as possible contributors to the inflammatory response by virtue of their participation in L-selectin ligand biosynthesis. Selective inhibitors would facilitate their functional elucidation and might provide leads for antiinflammatory therapy. Here we investigate the critical elements of a disaccharide substrate that are required for recognition by CHST2 and HEC-GlcNAc6ST. A panel of disaccharide analogues, bearing modifications to the pyranose rings and aglycon substituents, were synthesized and screened for substrate activity with each enzyme. Both GlcNAc-6-sulfotransferases required the 2-N-acetamido and 4-hydroxyl groups of a terminal GlcNAc residue for conversion to product. Both enzymes tolerated modifications to the reducing terminal pyranose. Key differences in recognition of an amide group in the aglycon substituent were observed, providing the basis for future glycomimetic inhibitor design.

  DOI：

  10.1021/ja001224k
• 作为产物：
  描述：

  2-N-p-nitrobenzyloxycarbonyl-1,3,4,6-tetra-O-acetyl-D-glucosamine 在 三氟化硼乙醚 、 potassium carbonate 、 苄胺 作用下， 以 四氢呋喃 、 二氯甲烷 为溶剂， 反应 19.0h， 生成 Acetic acid (2R,3S,4R,5R,6R)-3-acetoxy-2-acetoxymethyl-5-(4-nitro-benzyloxycarbonylamino)-6-((2R,3S,4S,5R,6S)-3,4,5-triacetoxy-6-octyloxy-tetrahydro-pyran-2-ylmethoxy)-tetrahydro-pyran-4-yl ester
  参考文献：
  名称：

  Differential Carbohydrate Recognition of Two GlcNAc-6-sulfotransferases with Possible Roles in L-Selectin Ligand Biosynthesis

  摘要：

  Two human GlcNAc-6-sulfotransferases, CHST2 and HEC-GlcNAc6ST, have been recently identified as possible contributors to the inflammatory response by virtue of their participation in L-selectin ligand biosynthesis. Selective inhibitors would facilitate their functional elucidation and might provide leads for antiinflammatory therapy. Here we investigate the critical elements of a disaccharide substrate that are required for recognition by CHST2 and HEC-GlcNAc6ST. A panel of disaccharide analogues, bearing modifications to the pyranose rings and aglycon substituents, were synthesized and screened for substrate activity with each enzyme. Both GlcNAc-6-sulfotransferases required the 2-N-acetamido and 4-hydroxyl groups of a terminal GlcNAc residue for conversion to product. Both enzymes tolerated modifications to the reducing terminal pyranose. Key differences in recognition of an amide group in the aglycon substituent were observed, providing the basis for future glycomimetic inhibitor design.

  DOI：

  10.1021/ja001224k