thiazole orange pentanoic acid succinimidyl ester | 1056651-26-3

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: thiazole orange pentanoic acid succinimidyl ester
• 英文别名: (2,5-Dioxopyrrolidin-1-yl) 5-[2-[(1-methylquinolin-1-ium-4-yl)methylidene]-1,3-benzothiazol-3-yl]pentanoate
• CAS: 1056651-26-3
• 化学式: C₂₇H₂₆N₃O₄S
• 分子量: 488.587
• InChiKey: OMCBCUXZGCKSDF-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.4
• 重原子数：
  35
• 可旋转键数：
  8
• 环数：
  5.0
• sp3杂化的碳原子比例：
  0.26
• 拓扑面积：
  96.1
• 氢给体数：
  0
• 氢受体数：
  6

文献信息

• COMPOUND, NUCLEIC ACID, LABELING SUBSTANCE, AND DETECTION METHOD
  申请人：KABUSHIKI KAISHA DNAFORM

  公开号：US20150252070A1

  公开（公告）日：2015-09-10

  The present invention provides a compound represented by the following chemical formula (I); a tautomer or stereoisomer of the compound; or a salt of the compound, the tautomer, or the stereoisomer. In the chemical formula (I), R 1 and R 2 are each a Group 1 element or a protecting group of an amino group and may be identical to or different from each other, or alternatively, R 1 and R 2 together may form a protecting group of an amino group. R 3 is a Group 1 element or a protecting group of a hydroxy group. R 4 is a Group 1 element or —PR 5 R 6 R 7 R 8 (R 5 , R 6 , R 7 , and R 8 are each a Group 1 element, a lone electron pair, a Group 16 element, a Group 17 element, or a protecting group of a phosphorus atom, and may be identical to or different from each other). J is a hydrogen atom or an arbitrary atomic group, A is a hydrogen atom, a hydroxy group, an alkyl group, an aralkyl group, an alkoxy group, an electron-withdrawing group, a silylene group, or a sulfide group, or alternatively, J and A together may form a linker. L is a single bond or a linker (a linking atom or a linking atomic group), the main chain length (the number of main chain atoms) of the linker is arbitrary, L may or may not contain each of C, N, O, S, P, and Si in the main chain, and may or may not contain each of a single bond, a double bond, a triple bond, an amide bond, an ester bond, a disulfide bond, an imino group, an ether bond, a thioether bond, and a thioester bond in the main chain. Z is an atomic group that can form a peptide bond with a labeling compound, or is an atom or atomic group including a label.

  本发明提供了以下化学式（I）所代表的化合物；该化合物的互变异构体或立体异构体；或者该化合物、互变异构体或立体异构体的盐。在化学式（I）中，R1和R2分别是第1族元素或氨基的保护基，它们可以相同也可以不同，或者R1和R2可以一起形成氨基的保护基。R3是第1族元素或羟基的保护基。R4是第1族元素或-PR5R6R7R8（R5、R6、R7和R8分别是第1族元素、孤立电子对、第16族元素、第17族元素或磷原子的保护基，它们可以相同也可以不同）。J是氢原子或任意的原子基团，A是氢原子、羟基、烷基、芳基烷基、烷氧基、吸电子基团、硅烷基团或硫化物基团，或者J和A可以一起形成连接基团。L是单键或连接基团（连接原子或连接原子基团），连接基团的主链长度（主链原子数）是任意的，L可能包含或不包含主链中的C、N、O、S、P和Si，也可能包含或不包含主链中的单键、双键、三键、酰胺键、酯键、二硫键、亚胺基团、醚键、硫醚键和硫酯键。Z是能与标记化合物形成肽键的原子基团，或者是包括标记的原子或原子基团。
• NUCLEIC ACID PROBE, METHOD FOR DESIGNING NUCLEIC ACID PROBE, AND METHOD FOR DETECTING TARGET SEQUENCE
  申请人：KABUSHIKI KAISHA DNAFORM

  公开号：US20150203902A1

  公开（公告）日：2015-07-23

  The present invention provides a nucleic acid probe that can achieve high detection sensitivity and high specificity in mutation detection, mismatch detection, etc. by the PCR method, a method for designing such a nucleic acid probe, and a method for detecting a target sequence. The nucleic acid probe includes a nucleic acid molecule, and the nucleic acid molecule includes a plurality of fluorescent dye moieties that exhibit an excitonic effect. At least two of the fluorescent dye moieties that exhibit an excitonic effect are bound to the same base or two adjacent bases in the nucleic acid molecule with each fluorescent dye moiety being bound via a linker (a linking atom or a linking atomic group). The extension-side end of the nucleic acid molecule is chemically modified, thereby preventing an extension reaction of the nucleic acid molecule.

  本发明提供了一种核酸探针，该探针可以通过PCR方法在突变检测、错配检测等方面实现高检测灵敏度和高特异性，以及设计这种核酸探针的方法和检测目标序列的方法。该核酸探针包括一个核酸分子，该核酸分子包括多个表现出激子效应的荧光染料基团。至少两个表现出激子效应的荧光染料基团与核酸分子中的同一碱基或相邻的两个碱基结合，每个荧光染料基团通过连接物（连接原子或连接原子团）结合。核酸分子的延伸侧端经过化学修饰，从而防止核酸分子的扩增反应。