4-[(1E,3E)-3-(3-甲基-1,3-苯并噻唑-2(3H)-亚基)-1-丙烯-1-基]-1-[3-(三甲基铵基)丙基]喹啉鎓二碘化物

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 中文名称: 4-[(1E,3E)-3-(3-甲基-1,3-苯并噻唑-2(3H)-亚基)-1-丙烯-1-基]-1-[3-(三甲基铵基)丙基]喹啉鎓二碘化物
• 英文名称: TO-PRO3 iodide
• 英文别名: trimethyl-[3-[4-[3-(3-methyl-1,3-benzothiazol-2-ylidene)prop-1-enyl]quinolin-1-ium-1-yl]propyl]azanium;diiodide
• 化学式: C26H31I2N3S
• 分子量: 671.4
• InChiKey: QHNORJFCVHUPNH-UHFFFAOYSA-L

计算性质

• 辛醇/水分配系数(LogP)：
  -0.67
• 重原子数：
  32
• 可旋转键数：
  6
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.27
• 拓扑面积：
  32.4
• 氢给体数：
  0
• 氢受体数：
  4

文献信息

• Blood analyzer and method for determining existence and nonexistence of lymphoblasts in blood sample
  申请人：Sysmex Corporation

  公开号：EP2237019A2

  公开（公告）日：2010-10-06

  The invention provides a blood analyzer (1) comprising a blood sample supply section, a sample preparation section (22) for preparing an assay sample by mixing the blood sample with a nucleic acid-staining fluorescent dye, a light source for irradiating the assay sample, an optical detecting section (23) for receiving fluorescence emitted from the irradiated assay sample and a controller for performing operations comprising detecting a cell group comprising lymphoblasts on the basis of the fluorescence received by the optical detecting section (23), and outputting an information on an appearance of the lymphoblasts in the blood sample, as well as a method for determining the existence and nonexistence of lymphoblasts in a blood sample.

  本发明提供了一种血液分析仪（1），包括一个血液样本供应部分、一个用于通过将血液样本与核酸染色荧光染料混合来制备检测样本的样本制备部分（22）、一个用于照射检测样本的光源、光学检测部分（23），用于接收从照射后的检测样本发出的荧光；控制器，用于执行包括根据光学检测部分（23）接收到的荧光检测包括淋巴母细胞在内的细胞群、输出血液样本中淋巴母细胞的外观信息等操作，以及确定血液样本中是否存在淋巴母细胞的方法。
• METHOD FOR CLASSIFYING/COUNTING LEUKOCYTES, REAGENT KIT FOR CLASSIFYING LEUKOCYTES, AND REAGENT FOR CLASSIFYING LEUKOCYTES
  申请人：Sysmex Corporation

  公开号：EP2703812A1

  公开（公告）日：2014-03-05

  The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The method for classifying and counting leukocytes comprises the steps of: preparing a measurement sample by mixing a biological sample; a first reagent for staining nucleic acid; and a second reagent containing cationic and nonionic surfactants for lysing erythrocytes and damaging cell membranes of leukocytes so as to be permeable to a fluorescent dye and an aromatic organic acid at a concentration of not less than 20 mM and not more than 50 mM; obtaining scattered light information and fluorescence information generated by applying light to the measurement sample; and based on the obtained scattered light information and fluorescence information, classifying the leukocytes in the biological sample and detecting blast cells and atypical lymphocytes by differentiating therebetween, wherein when the second reagent contains the aromatic organic acid at a concentration of not less than 20 mM and less than 30 mM, the second reagent has pH of not lower than 5.5 and not higher than 6.4, and when the concentration is not less than 30 mM and not more than 50 mM, the pH is not lower than 5.5 and not higher than 7.0.

  本发明提供了一种白细胞分类和计数方法，该方法可对正常白细胞进行分类和计数，并可区分突变细胞和非典型淋巴细胞。白细胞分类和计数方法包括以下步骤混合生物样本、用于核酸染色的第一试剂和第二试剂，后者含有阳离子和非离子表面活性剂，用于裂解红细胞和破坏白细胞的细胞膜，从而使荧光染料和芳香族有机酸的渗透性不低于 20 mM 且不高于 50 mM；获取散射光信息和通过对测量样本施加光而产生的荧光信息；根据所获得的散射光信息和荧光信息，对生物样品中的白细胞进行分类，并通过区分白细胞和非典型淋巴细胞来检测爆炸细胞和非典型淋巴细胞，其中，当第二试剂含有浓度不小于20 mM且小于30 mM的芳香族有机酸时，第二试剂的pH值不低于5.5且不高于6.4，而当浓度不低于30 mM且不高于50 mM时，pH值不低于5.5且不高于7.0。
• Method for analyzing blood cells and blood cell analyzer
  申请人：SYSMEX CORPORATION

  公开号：EP2735859A2

  公开（公告）日：2014-05-28

  A method for analyzing blood cells includes: preparing a measurement specimen for classifying white blood cells, by mixing a sample with a reagent in order to hemolyze red blood cells contained in the sample and to fluorescently stain nucleic acid in white blood cells contained in the sample; detecting, by flowing the prepared measurement specimen in a flow cell, fluorescence emitted from each blood cell in the measurement specimen and two types of scattered light at respective different angles, and obtaining a fluorescence signal and two types of scattered light signals; and classifying the white blood cells into at least four groups and detecting neoplastic lymphocytes, by performing analysis using at least three types of parameters based on the obtained fluorescence signal and two types of scattered light signals.

  一种分析血细胞的方法包括通过将样品与试剂混合以溶解样品中所含的红细胞并对样品中所含的白细胞中的核酸进行荧光染色，制备用于白细胞分类的测量样本；通过将制备好的测量样本在流动池中流动，检测测量样本中每个血细胞发出的荧光以及在各自不同角度的两种散射光，并获得荧光信号和两种散射光信号；根据获得的荧光信号和两种散射光信号，使用至少三种参数进行分析，将白细胞分为至少四组，并检测出肿瘤性淋巴细胞。
• SPECIMEN ANALYZING METHOD, SPECIMEN ANALYZER, AND REAGENT
  申请人：SYSMEX CORPORATION

  公开号：EP3249384A1

  公开（公告）日：2017-11-29

  A specimen analyzing method of an embodiment includes: preparing a measurement specimen from a biological specimen containing blood cells by staining, with a nucleic acid staining fluorescent dye, nucleic acids contained in neutrophils not having released neutrophil extracellular traps; obtaining optical information including fluorescence information by irradiating the measurement specimen with light; and detecting, as neutrophil extracellular traps on the basis of the optical information, particles having lower fluorescence intensity than fluorescence intensity obtained from the neutrophils not having released neutrophil extracellular traps.

  一个实施例的标本分析方法包括通过使用核酸染色荧光染料对未释放中性粒细胞胞外捕获物的中性粒细胞中所含的核酸进行染色，从含有血细胞的生物标本中制备测量标本；通过用光照射测量标本，获得包括荧光信息在内的光学信息；根据光学信息检测出荧光强度低于未释放中性粒细胞胞外捕获物的中性粒细胞的荧光强度的颗粒，将其作为中性粒细胞胞外捕获物。
• SAMPLE PREPARING APPARATUS AND SAMPLE PREPARING METHOD
  申请人：SYSMEX CORPORATION

  公开号：EP3454038A1

  公开（公告）日：2019-03-13

  [Object] To accurately analyze measurement target particles in each specimen, while reducing variation in dispensing amounts of specimens which is caused by different viscosities of the respective specimens. [Solution] A sample preparing apparatus 1 includes: a measurement section 2 configured to measure a specimen obtained from a specimen container 10, and obtain viscosity information relating to viscosity of the specimen; a sample preparation section 3 configured to prepare a measurement sample by aspirating the specimen from the specimen container 10, discharging the specimen into a mixing container 11, and mixing the specimen with a labeling substance in the mixing container 11; and a control section 4 configured to determine, on the basis of viscosity information, at least one of an aspiration condition for aspirating the specimen and a discharge condition for discharging the specimen, and control the sample preparation section 3.

  [目的]准确分析各试样中的测量目标颗粒，同时减少因各试样粘度不同而导致的试样分配量的变化。 [解决方案] 样品制备装置 1 包括测量部分 2，用于测量从试样容器 10 中获得的试样，并获得与试样粘度相关的粘度信息； 试样制备部分 3，用于通过从试样容器 10 中吸入试样、将试样排放到混合容器 11 中并将试样与混合容器 11 中的标记物质混合来制备测量试样；控制部分 4 配置用于根据粘度信息确定吸入试样的吸入条件和排出试样的排出条件中的至少一个条件，并控制样品制备部分 3。