名称:
Immobilization of fluorous oligosaccharide recognized by influenza virus on polytetrafluoroethylene filter
摘要:
The lactoside with PEG-fluorous tag was introduced to BHK-21(C-13) cells to generate a GM3-type oligosaccharide (Sia alpha 2-3Gal beta 1-4Glc). The GM3-type oligosaccharide obtained was easily immobilized by spotting onto commercially available polytetrafluoroethylene (PTFE) filter through non-covalent fluorous affinity and simply assessed by dot blot method using the interaction of carbohydrate- with proteins which recognize sialic acid such as virus membrane proteins. (C) 2011 Elsevier Ltd. All rights reserved.
DOI:
10.1016/j.bmcl.2011.11.057