4-[(Z)-(3-甲基-1,3-苯并噻唑-2(3H)-亚基)甲基]-1-[3-(三甲基铵基)丙基]喹啉鎓二碘化物 | 157199-59-2

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 中文名称: 4-[(Z)-(3-甲基-1,3-苯并噻唑-2(3H)-亚基)甲基]-1-[3-(三甲基铵基)丙基]喹啉鎓二碘化物
• 英文名称: To-Pro-1
• 英文别名: trimethyl-[3-[4-[(3-methyl-1,3-benzothiazol-2-ylidene)methyl]quinolin-1-ium-1-yl]propyl]azanium;diiodide
• CAS: 157199-59-2
• 化学式: C24H29I2N3S
• 分子量: 645.4
• InChiKey: DPXHITFUCHFTKR-UHFFFAOYSA-L

物化性质

• 熔点：
  >250℃
• 溶解度：
  溶于二甲基亚砜
• 最大波长(λmax)：
  515 nm

计算性质

• 辛醇/水分配系数(LogP)：
  -1.23
• 重原子数：
  30
• 可旋转键数：
  5
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.29
• 拓扑面积：
  32.4
• 氢给体数：
  0
• 氢受体数：
  4

文献信息

• Blood analyzer and method for determining existence and nonexistence of lymphoblasts in blood sample
  申请人：Sysmex Corporation

  公开号：EP2237019A2

  公开（公告）日：2010-10-06

  The invention provides a blood analyzer (1) comprising a blood sample supply section, a sample preparation section (22) for preparing an assay sample by mixing the blood sample with a nucleic acid-staining fluorescent dye, a light source for irradiating the assay sample, an optical detecting section (23) for receiving fluorescence emitted from the irradiated assay sample and a controller for performing operations comprising detecting a cell group comprising lymphoblasts on the basis of the fluorescence received by the optical detecting section (23), and outputting an information on an appearance of the lymphoblasts in the blood sample, as well as a method for determining the existence and nonexistence of lymphoblasts in a blood sample.

  本发明提供了一种血液分析仪（1），包括一个血液样本供应部分、一个用于通过将血液样本与核酸染色荧光染料混合来制备检测样本的样本制备部分（22）、一个用于照射检测样本的光源、光学检测部分（23），用于接收从照射后的检测样本发出的荧光；控制器，用于执行包括根据光学检测部分（23）接收到的荧光检测包括淋巴母细胞在内的细胞群、输出血液样本中淋巴母细胞的外观信息等操作，以及确定血液样本中是否存在淋巴母细胞的方法。
• METHOD FOR CLASSIFYING/COUNTING LEUKOCYTES, REAGENT KIT FOR CLASSIFYING LEUKOCYTES, AND REAGENT FOR CLASSIFYING LEUKOCYTES
  申请人：Sysmex Corporation

  公开号：EP2703812A1

  公开（公告）日：2014-03-05

  The present invention provides a method for classifying and counting leukocytes which allows classification and count of normal leukocytes as well as discrimination between blast cells and atypical lymphocytes. The method for classifying and counting leukocytes comprises the steps of: preparing a measurement sample by mixing a biological sample; a first reagent for staining nucleic acid; and a second reagent containing cationic and nonionic surfactants for lysing erythrocytes and damaging cell membranes of leukocytes so as to be permeable to a fluorescent dye and an aromatic organic acid at a concentration of not less than 20 mM and not more than 50 mM; obtaining scattered light information and fluorescence information generated by applying light to the measurement sample; and based on the obtained scattered light information and fluorescence information, classifying the leukocytes in the biological sample and detecting blast cells and atypical lymphocytes by differentiating therebetween, wherein when the second reagent contains the aromatic organic acid at a concentration of not less than 20 mM and less than 30 mM, the second reagent has pH of not lower than 5.5 and not higher than 6.4, and when the concentration is not less than 30 mM and not more than 50 mM, the pH is not lower than 5.5 and not higher than 7.0.

  本发明提供了一种白细胞分类和计数方法，该方法可对正常白细胞进行分类和计数，并可区分突变细胞和非典型淋巴细胞。白细胞分类和计数方法包括以下步骤混合生物样本、用于核酸染色的第一试剂和第二试剂，后者含有阳离子和非离子表面活性剂，用于裂解红细胞和破坏白细胞的细胞膜，从而使荧光染料和芳香族有机酸的渗透性不低于 20 mM 且不高于 50 mM；获取散射光信息和通过对测量样本施加光而产生的荧光信息；根据所获得的散射光信息和荧光信息，对生物样品中的白细胞进行分类，并通过区分白细胞和非典型淋巴细胞来检测爆炸细胞和非典型淋巴细胞，其中，当第二试剂含有浓度不小于20 mM且小于30 mM的芳香族有机酸时，第二试剂的pH值不低于5.5且不高于6.4，而当浓度不低于30 mM且不高于50 mM时，pH值不低于5.5且不高于7.0。
• Method for analyzing blood cells and blood cell analyzer
  申请人：SYSMEX CORPORATION

  公开号：EP2735859A2

  公开（公告）日：2014-05-28

  A method for analyzing blood cells includes: preparing a measurement specimen for classifying white blood cells, by mixing a sample with a reagent in order to hemolyze red blood cells contained in the sample and to fluorescently stain nucleic acid in white blood cells contained in the sample; detecting, by flowing the prepared measurement specimen in a flow cell, fluorescence emitted from each blood cell in the measurement specimen and two types of scattered light at respective different angles, and obtaining a fluorescence signal and two types of scattered light signals; and classifying the white blood cells into at least four groups and detecting neoplastic lymphocytes, by performing analysis using at least three types of parameters based on the obtained fluorescence signal and two types of scattered light signals.

  一种分析血细胞的方法包括通过将样品与试剂混合以溶解样品中所含的红细胞并对样品中所含的白细胞中的核酸进行荧光染色，制备用于白细胞分类的测量样本；通过将制备好的测量样本在流动池中流动，检测测量样本中每个血细胞发出的荧光以及在各自不同角度的两种散射光，并获得荧光信号和两种散射光信号；根据获得的荧光信号和两种散射光信号，使用至少三种参数进行分析，将白细胞分为至少四组，并检测出肿瘤性淋巴细胞。
• METHOD FOR DETERMINING ABNORMALITY IN PARTICLE ANALYZER AND PARTICLE ANALYZER
  申请人：SYSMEX CORPORATION

  公开号：EP3002581A1

  公开（公告）日：2016-04-06

  Disclosed is a method for determining abnormality in a particle analyzer (10). The method includes: staining first control particles but not staining second control particles which emit fluorescence; irradiating with light the first control particles and the second control particles flowing in a flow cell, and detecting fluorescence from the first control particles and the second control particles; obtaining a first management value indicating a detection result of the fluorescence emitted from the first control particles and a second management value indicating a detection result of the fluorescence emitted from the second control particles; and determining abnormality in the staining step, based on a value calculated from the first management value and the second management value or a ratio between the first management value and the second management value.

  所公开的是一种用于确定颗粒分析仪（10）异常的方法。该方法包括对发射荧光的第一对照粒子进行染色，但不对第二对照粒子进行染色；用光照射在流动池中流动的第一对照粒子和第二对照粒子，并检测来自第一对照粒子和第二对照粒子的荧光；获得表示第一对照粒子发出的荧光的检测结果的第一管理值和表示第二对照粒子发出的荧光的检测结果的第二管理值；根据从第一管理值和第二管理值计算出的值或第一管理值和第二管理值之间的比率，确定染色步骤中的异常。
• TUMOR CELL ANALYZER AND TUMOR CELL ANALYZING METHOD
  申请人：SYSMEX CORPORATION

  公开号：EP3141884A1

  公开（公告）日：2017-03-15

  Disclosed is a cell analyzer comprising: a flow cell through which a measurement specimen containing a body fluid flows; a light emission unit that applies light onto the measurement specimen flowing through the flow cell; a light detection unit that detects forward scattered light generated from cells in the measurement specimen to which the light is applied; an analysis unit that detects the cells in the body fluid based on a forward scattered light signal detected by the light detection unit; and an output unit, wherein the analysis unit outputs information about tumor cells in the body fluid to the output unit, based on forward scattered light signal intensity and forward scattered light signal width.

  所公开的细胞分析仪包括一个流动池，含有体液的测量样本流经该流动池；一个光发射装置，将光照到流经流动池的测量样本上；一个光检测装置，检测光照到的测量样本中的细胞产生的前向散射光；分析单元，根据光检测单元检测到的前向散射光信号检测体液中的细胞；以及输出单元，其中分析单元根据前向散射光信号强度和前向散射光信号宽度向输出单元输出体液中肿瘤细胞的信息。