Mixed SAMs and MALDI–ToF MS: Preparation of N-glycosylamine derivative and thioctic acid methyl ester bearing 1,2-dithiolane groups and detection of enzymatic reaction on Au
摘要:
Herein, we report an enzymatic galactosylation reaction of beta-glucopyranosylamide 4 and thioctic acid methyl ester 5 bearing 1,2-dithiolane groups to form a new system of mixed self-assembled monolayers (SAMs) on gold. Characterization of the enzymatic activity was conveniently achieved by mass spectrometry. (C) 2011 Elsevier Inc. All rights reserved.