4-[(3-Methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]-1-propylquinolin-1-ium | 849592-32-1

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: 4-[(3-Methyl-1,3-benzothiazol-2(3H)-ylidene)methyl]-1-propylquinolin-1-ium
• 英文别名: 3-methyl-2-[(1-propylquinolin-1-ium-4-yl)methylidene]-1,3-benzothiazole
• CAS: 849592-32-1
• 化学式: C21H21N2S+
• 分子量: 333.5
• InChiKey: SRUFJYGZWRFDHS-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  5.6
• 重原子数：
  24
• 可旋转键数：
  3
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.19
• 拓扑面积：
  32.4
• 氢给体数：
  0
• 氢受体数：
  2

文献信息

• Detection of immobilised nucleic acid
  申请人：Molecular Probes Inc.

  公开号：EP1988177A1

  公开（公告）日：2008-11-05

  The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.

  本发明提供了利用非对称氰基染料（噻唑橙的衍生物）、染色溶液和特征为基本无遗传毒性的精选致氟化合物来确定固定化核酸存在的方法。这些方法包括将核酸、单链或双链 DNA、RNA 或它们的组合固定在固体或半固体支持物上，将固定的核酸与不对称氰基染料化合物接触，然后用适当的波长照射固定的核酸，从而确定核酸的存在。氰基染料化合物通常存在于水性染色溶液中，该溶液由染料化合物和三醋酸盐或三硼酸盐缓冲液组成，其中的溶液有利于染料化合物与固定化核酸的接触。固体或半固体支持物通常选自聚合物凝胶、膜、阵列、玻璃珠、玻璃载玻片和聚合物微粒组成的组。聚合物凝胶最好是琼脂糖或聚丙烯酰胺。使用无遗传毒性化合物的方法是对使用溴化乙锭的常用方法的改进，本方法保留了溴化乙锭的优点，使用方便，成本低，但没有已知诱变剂的缺点，需要特殊的处理和废物处理程序。
• DETECTION OF IMMOBILIZED NUCLEIC ACID
  申请人：Molecular Probes Inc.

  公开号：EP1668162B1

  公开（公告）日：2009-04-08
• Detection of Immobilized Nucleic Acid
  申请人：LIFE TECHNOLOGIES CORPORATION

  公开号：US20140076726A1

  公开（公告）日：2014-03-20

  The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid.
• METHOD FOR VISUALISING BIOMOLECULES, SUCH AS PROTEINS OR NUCLEIC ACIDS, WITH THE UNAIDED EYE, WITHOUT NEEDING TO USE POTENTIALLLY TOXIC COMPOUNDS, EXPOSURE TO ULTRAVIOLET (UV) LIGHT OR FLUORESCENCE
  申请人：UNIVERSIDAD DE CHILE

  公开号：US20170356919A1

  公开（公告）日：2017-12-14

  The present invention relates to a method of visualizing biomolecules, having the steps of: a) providing a sample of immobilized biomolecules in a matrix and carry on the electrophoresis process; b) incubating the matrix of step a) in a solution containing a cyanine-derived molecule, for a time of 5 to 60 minutes, at room temperature, in a container preventing exposure to light, shaking the container at less of 75 rpm; c) transferring the matrix from step b) to a container with a solution having: at least one tetrazolium salt and incubating for a time of 15 to 120 minutes at room temperature under light exposure; d) removing the matrix with immobilized biomolecules from the previous step and washing with distilled water; and e) visualizing directly by the naked eye the biomolecules immobilized in the matrix.
• US7727716B2
  申请人：——

  公开号：US7727716B2

  公开（公告）日：2010-06-01