Uridylyl-3'->1-O-naphthalin | 13090-05-6

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: Uridylyl-3'->1-O-naphthalin
• 英文别名: [(2R,3S,4R,5R)-5-(2,4-dioxopyrimidin-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] naphthalen-1-yl hydrogen phosphate
• CAS: 13090-05-6
• 化学式: C₁₉H₁₉N₂O₉P
• 分子量: 450.342
• InChiKey: CARALYKQJQFQHT-VDHUWJSZSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.3
• 重原子数：
  31
• 可旋转键数：
  6
• 环数：
  4.0
• sp3杂化的碳原子比例：
  0.26
• 拓扑面积：
  155
• 氢给体数：
  4
• 氢受体数：
  9

文献信息

• A method for carrying out non-isotopic immunoassays, labeled analytes and kits for use in such assays
  申请人：Baker Instruments Corporation

  公开号：EP0062277A1

  公开（公告）日：1982-10-13

  A highly sensitive, immunoassay method for determining the amount of an analyte in a sample containing a known analyte in an unknown concentration is provided. Said sample, a polypeptide-labeled analog of the analyte, an antibody specific for said analyte, a polypeptide partner capable of non-covalently binding with the polypeptide-labeled analyte to form a complex having catalytic activity, and a substrate capable of being converted to a reporter molecule by the catalytic activity of said complex are brought together in a medium. The polypeptide-labeled analyte analog is capable of competitively binding to the antibody and the polypeptide partner, the antibody inhibiting the formation of a catalytically active complex in the absence of analyte, and the concentrations of the antibody, polypeptide partner and polypeptide-labeled analyte are such as to cause varying amounts of analyte, to be directly related to the conversion of the substrate to the reporter molecule. Conversion of the substrate to the reporter molecule is then determined, and compared to conversions of substrate to reporter molecule obtained with known concentrations of the analyte.

  本发明提供了一种高灵敏度的免疫测定方法，用于测定含有未知浓度已知分析物的样品中分析物的含量。将所述样品、多肽标记的分析物类似物、针对所述分析物的特异性抗体、能够与多肽标记的分析物非共价结合形成具有催化活性的复合物的多肽伙伴，以及能够在所述复合物的催化活性作用下转化为报告分子的底物一起置于培养基中。多肽标记的分析物类似物能够竞争性地与抗体和多肽伙伴结合，抗体在没有分析物的情况下抑制具有催化活性的复合物的形成，抗体、多肽伙伴和多肽标记的分析物的浓度使分析物的量变化与底物向报告分子的转化直接相关。然后测定底物到报告分子的转化率，并与已知浓度的分析物得到的底物到报告分子的转化率进行比较。
• A method for preparing a mononucleotide-3'-phosphodiester-based substrate
  申请人：Baker Instruments Corporation

  公开号：EP0061760B1

  公开（公告）日：1988-03-02
• US4378428A
  申请人：——

  公开号：US4378428A

  公开（公告）日：1983-03-29
• US4529796A
  申请人：——

  公开号：US4529796A

  公开（公告）日：1985-07-16
• US4785080A
  申请人：——

  公开号：US4785080A

  公开（公告）日：1988-11-15