| 1039152-07-2

• 分子结构分类: 有机化合物 - 有机氧化合物
• CAS: 1039152-07-2
• 化学式: C₄₆H₆₀N₉O₁₈P
• 分子量: 1058.01
• InChiKey: ADWXVEDDJCABCT-UXGMEINLSA-N

反应信息

• 作为反应物：
  描述：

  在 ammonium hydroxide 作用下， 以 甲醇 为溶剂， 反应 14.0h， 生成
  参考文献：
  名称：

  Photocleavable Initiator Nucleotide Substrates for an Aldolase Ribozyme

  摘要：

  We have previously reported the in vitro selection of a ribozyme that catalyzes an aldol reaction between a levulinic amide aldol donor and a benzaldehyde substrate. The selection scheme involved the priming of the RNA library with a levulinic amide aldol donor group that was introduced via transcription priming in the presence of a modified guanosine mononucleotide derivative. Here we provide a detailed description of the synthesis of the ribozyme substrates and the substrate oligonucleotides used for its isolation and characterization. The aldol donor group was attached to the phosphate moiety of guanosine monophosphate via a photocleavable linker molecule. This initiator nucleotide was efficiently incorporated into RNA molecules of differing sizes and composition by transcription priming with T7 RNA polymerase. With this method modified RNA oligonucleotides as small as a 6-mer sequence can be generated. A temperature profile of the intermolecular reaction indicates that the modified RNA hexamer binds the ribozyme largely by Watson-Crick pairing and only to a minor extent via the non-RNA moiety, whereas the ribozyme appears to have evolved a specific binding site for the aldehyde substrate.

  DOI：

  10.1021/jo800639p
• 作为产物：
  描述：

  在 吡啶 、 水 、 碘 作用下， 以 乙腈 为溶剂， 反应 0.58h， 以0.926 g的产率得到
  参考文献：
  名称：

  Photocleavable Initiator Nucleotide Substrates for an Aldolase Ribozyme

  摘要：

  We have previously reported the in vitro selection of a ribozyme that catalyzes an aldol reaction between a levulinic amide aldol donor and a benzaldehyde substrate. The selection scheme involved the priming of the RNA library with a levulinic amide aldol donor group that was introduced via transcription priming in the presence of a modified guanosine mononucleotide derivative. Here we provide a detailed description of the synthesis of the ribozyme substrates and the substrate oligonucleotides used for its isolation and characterization. The aldol donor group was attached to the phosphate moiety of guanosine monophosphate via a photocleavable linker molecule. This initiator nucleotide was efficiently incorporated into RNA molecules of differing sizes and composition by transcription priming with T7 RNA polymerase. With this method modified RNA oligonucleotides as small as a 6-mer sequence can be generated. A temperature profile of the intermolecular reaction indicates that the modified RNA hexamer binds the ribozyme largely by Watson-Crick pairing and only to a minor extent via the non-RNA moiety, whereas the ribozyme appears to have evolved a specific binding site for the aldehyde substrate.

  DOI：

  10.1021/jo800639p