m-nitrophenyl-α-D-galactopyranoside | 56140-66-0

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: m-nitrophenyl-α-D-galactopyranoside
• 英文别名: (3-nitro-phenyl)-α-D-glucopyranoside；(3-Nitro-phenyl)-α-D-glucopyranosid；<3-Nitro-phenol>-α-D-glucopyranosid；m-Nitrophenyl-α-D-glucopyranosid；(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-(3-nitrophenoxy)oxane-3,4,5-triol
• CAS: 56140-66-0
• 化学式: C₁₂H₁₅NO₈
• 分子量: 301.253
• InChiKey: VCCMGHVCRFMITI-ZIQFBCGOSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.5
• 重原子数：
  21
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  145
• 氢给体数：
  4
• 氢受体数：
  8

反应信息

• 作为反应物：
  描述：

  m-nitrophenyl-α-D-galactopyranoside 在 Ganoderma lucidium α-galactosidase 作用下， 以 various solvent(s) 为溶剂， 生成 D-葡萄糖 、 间硝基苯酚
  参考文献：
  名称：

  Purification and Characterization of Thermostable α-Galactosidase fromGanoderma lucidum

  摘要：

  通过硫酸铵沉淀以及 DEAE-Sephadex 和 Con A-Sepharose 柱层析，从灵芝新鲜子实体中纯化了 α-半乳糖苷酶。纯化后的酶在聚丙烯酰胺凝胶电泳中呈均匀分布。其 N 端氨基酸序列与醋酸莫蒂尔氏菌 α-半乳糖苷酶相似。经 SDS 聚丙烯酰胺凝胶电泳测定，该酶的分子质量约为 56 kDa；经凝胶过滤柱层析测定，该酶的分子质量约为 249 kDa。最适 pH 值和温度分别为 6.0 和 70°C。该酶在 70°C 下加热 30 分钟完全稳定。它能水解对硝基苯-α-D-吡喃半乳糖苷（Km=0.4 mM），但很少水解邻硝基苯-α-D-吡喃半乳糖苷。它还能水解三聚果糖、棉子糖和水苏糖。该酶催化了反式半乳糖基化反应，合成了三聚果糖。通过各种分析确认了该产物。

  DOI：

  10.1271/bbb.67.1485
• 作为产物：
  描述：

  3-nitrophenyl 2,3,4,6-tetra-O-acetyl-α-D-glucopyranoside 在 甲醇 、 barium methoxide 作用下， 生成 m-nitrophenyl-α-D-galactopyranoside
  参考文献：
  名称：

  Pigman, Journal of Research of the National Bureau of Standards (United States), 1944, vol. 33, p. 129,143

  摘要：

  DOI：

文献信息

• Plating media for the presumptive identification of the genus Shigella and the species Shigella sonnei and shigella boydii
  申请人：——

  公开号：US20030049718A1

  公开（公告）日：2003-03-13

  A solid growth plating medium in which Shigella organisms will grow and form colonies in the medium, and substantially, other microorganisms are inhibited or their colonies are differentiated from Shigella organisms. In one embodiment of the invention, colonies produced by Shigella appear with the color of the plating medium, usually a clear off-white color, that can be readily observed. In another embodiment, the fact that Shigella boydii and Shigella sonnei produce the enzyme alpha-galactosidase, but most Shigella dysenteriae and Shigella flexneri strains do not, is utilized with a chromogenic substrate to produce colonies of these microorganisms of a distinguishing color.

  一种固体生长电镀培养基，其中的志贺氏杆菌将在培养基中生长并形成菌落，而其他微生物基本上被抑制或其菌落与志贺氏杆菌区分开来。在本发明的一个实施方案中，由志贺氏杆菌产生的菌落与电镀培养基的颜色一起出现，通常是一种清晰的米白色，可以很容易地观察到。在另一个实施方案中，利用男孩志贺氏菌和松内志贺氏菌产生α-半乳糖苷酶，而大多数痢疾志贺氏菌和柔性志贺氏菌菌株不产生这种酶这一事实，用显色底物来产生这些微生物的菌落，使其具有明显的颜色。
• Yu, Jaehoon; Hsieh, Linda C.; Kochersperger, Lynn, Angewandte Chemie, 1994, vol. 106, # 3, p. 327 - 329
  作者：Yu, Jaehoon、Hsieh, Linda C.、Kochersperger, Lynn、Yonkovich, Shirlee、Stephans, James C.、et al.

  DOI：——

  日期：——
• US6764832B2
  申请人：——

  公开号：US6764832B2

  公开（公告）日：2004-07-20
• Pigman, Journal of Research of the National Bureau of Standards (United States), 1944, vol. 33, p. 129,143
  作者：Pigman

  DOI：——

  日期：——
• Purification and Characterization of Thermostable α-Galactosidase from<i>Ganoderma lucidum</i>
  作者：Thida SRIPUAN、Kazuhiro AOKI、Kenji YAMAMOTO、Dararat TONGKAO、Hidehiko KUMAGAI

  DOI：10.1271/bbb.67.1485

  日期：2003.1

  α-Galactosidase was purified from a fresh fruiting body of Ganoderma lucidum by precipitation with ammonium sulfate and column chromatographies with DEAE-Sephadex and Con A-Sepharose. The purified enzyme was homogeneous on polyacrylamide gel electrophoresis. Its N-terminal amino acid sequence was similar to that of Mortierella vinacea α-galactosidase. The molecular mass of the enzyme was about 56 kDa by SDS-polyacrylamide gel electrophoresis, and about 249 kDa by gel filtration column chromatography. The optimum pH and temperature were 6.0 and 70°C, respectively. The enzyme was fully stable to heating at 70°C for 30 min. It hydrolyzed p-nitrophenyl-α-D-galactopyranoside (Km=0.4 mM) but hydrolyzed little o-nitrophenyl-α-D-galactopyranoside. It also hydrolyzed melibiose, raffinose, and stachyose. The enzyme catalyzed the transgalactosylation reaction which synthesized melibiose. The product was confirmed by various analyses.

  通过硫酸铵沉淀以及 DEAE-Sephadex 和 Con A-Sepharose 柱层析，从灵芝新鲜子实体中纯化了 α-半乳糖苷酶。纯化后的酶在聚丙烯酰胺凝胶电泳中呈均匀分布。其 N 端氨基酸序列与醋酸莫蒂尔氏菌 α-半乳糖苷酶相似。经 SDS 聚丙烯酰胺凝胶电泳测定，该酶的分子质量约为 56 kDa；经凝胶过滤柱层析测定，该酶的分子质量约为 249 kDa。最适 pH 值和温度分别为 6.0 和 70°C。该酶在 70°C 下加热 30 分钟完全稳定。它能水解对硝基苯-α-D-吡喃半乳糖苷（Km=0.4 mM），但很少水解邻硝基苯-α-D-吡喃半乳糖苷。它还能水解三聚果糖、棉子糖和水苏糖。该酶催化了反式半乳糖基化反应，合成了三聚果糖。通过各种分析确认了该产物。