1-O-α-D-glucopyranosyl-β-D-fructofuranose | 90701-11-4

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: 1-O-α-D-glucopyranosyl-β-D-fructofuranose
• 英文别名: β-D-fructofuranosyl-α-D-glucopyranoside；trehalulose；1-O-alpha-D-glucopyranosyl-beta-D-fructofuranose；(2R,3S,4S,5R,6S)-2-(hydroxymethyl)-6-[[(2R,3S,4S,5R)-2,3,4-trihydroxy-5-(hydroxymethyl)oxolan-2-yl]methoxy]oxane-3,4,5-triol
• CAS: 90701-11-4
• 化学式: C₁₂H₂₂O₁₁
• 分子量: 342.3
• InChiKey: NMXLJRHBJVMYPD-IPFGBZKGSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -4.3
• 重原子数：
  23
• 可旋转键数：
  5
• 环数：
  2.0
• sp3杂化的碳原子比例：
  1.0
• 拓扑面积：
  190
• 氢给体数：
  8
• 氢受体数：
  11

反应信息

• 作为反应物：
  描述：

  1-O-α-D-glucopyranosyl-β-D-fructofuranose 在 sucrase; glucoamylase; maltase; isomaltase; mixture of 作用下， 反应 1.0h， 生成 D-葡萄糖
  参考文献：
  名称：

  EP1864670

  摘要：

  公开号：
• 作为产物：
  描述：

  蔗糖 生成 1-O-α-D-glucopyranosyl-β-D-fructofuranose
  参考文献：
  名称：

  Trehalulose Synthase Native and Carbohydrate Complexed Structures Provide Insights into Sucrose Isomerization

  摘要：

  Various diseases related to the overconsumption of sugar make a growing need for sugar substitutes. Because sucrose is an inexpensive and readily available D-glucose donor, the industrial potential for enzymatic synthesis of the sucrose isomers trehalulose and/or isomaltulose from sucrose is large. The product specificity of sucrose isomerases that catalyze this reaction depends essentially on the possibility for tautomerization of sucrose, which is required for trehalulose formation. For optimal use of the enzyme, targeting controlled synthesis of these functional isomers, it is necessary to minimize the side reactions. This requires an extensive analysis of substrate binding modes and of the specificity-determining sites in the structure. The 1.6-2.2-angstrom resolution three-dimensional structures of native and mutant complexes of a trehalulose synthase from Pseudomonas mesoacidophila MX-45 mimic successive states of the enzyme reaction. Combined with mutagenesis studies they give for the first time thorough insights into substrate recognition and processing and reaction specificities of these enzymes. Among the important outcomes of this study is the revelation of an aromatic clamp defined by Phe(256) and Phe(280) playing an essential role in substrate recognition and in controlling the reaction specificity, which is further supported by mutagenesis studies. Furthermore, this study highlights essential residues for binding the glucosyl and fructosyl moieties. The introduction of subtle changes informed by comparative three-dimensional structural data observed within our study can lead to fundamental modifications in the mode of action of sucrose isomerases and hence provide a template for industrial catalysts.

  DOI：

  10.1074/jbc.m704515200

文献信息

• A New Colorimetric Method for Determining the Isomerization Activity of Sucrose Isomerase
  作者：Sang-Eun PARK、Mee-Hyun CHO、Jin Kyu LIM、Jong-Sang KIM、Jeong Hwan KIM、Dae Young KWON、Cheon-Seok PARK

  DOI：10.1271/bbb.60509

  日期：2007.2.23

  A new colorimetric method for determining the isomerization activity of sucrose isomerase was developed. This colorimetric method is based on the enzymatic reactions of invertase and glucose oxidase-peroxidase (GOD-POD). The main scheme for assaying sucrose isomerase activity is to degrade sucrose in the reaction mixture to glucose and fructose by invertase and to detect the concentration of glucose

  建立了测定蔗糖异构酶异构化活性的新比色法。该比色法基于转化酶和葡萄糖氧化酶过氧化物酶（GOD-POD）的酶促反应。测定蔗糖异构酶活性的主要方案是通过转化酶将反应混合物中的蔗糖降解为葡萄糖和果糖，并检测使用GOD-POD产生的葡萄糖浓度。由葡萄糖的浓度计算出蔗糖异构酶的反应产物海藻糖和异麦芽酮糖的浓度。该方法可以快速而准确地确定蔗糖异构酶的异构化活性，而不受水解活性的抑制。
• Präparat zur Wirkstoffapplikation in Kleinsttröpfchenform
  申请人：Cevc, Gregor, Prof. Dr.

  公开号：EP0475160A1

  公开（公告）日：1992-03-18

  Die Erfindung betrifft ein Präparat zur Applikation von Wirkstoffen in Form kleinster, insbesondere mit einer membranartigen Hülle aus einer oder wenigen Lagen amphiphiler Moleküle bzw. mit einer amphiphilen Trägersubstanz versehenen Flüssigkeitströpfchen, insbesondere zum Transport des Wirkstoffes in und durch natürliche Barrieren und Konstriktionen wie Häute und dergleichen. Das Präparat weist einen Gehalt einer randaktiven Substanz auf, der bis zu 99 Mol.-% des Gehaltes dieser Substanz entspricht, durch den der Solubilisierungspunkt der Tröpfchen erreicht wird. Das Präparat eignet sich zur nichtinvasiven Verabreichung von Antidiabetica, insbesondere von Insulin. Die Erfindung betrifft außerdem ein Verfahren zur Herstellung solcher Präparate.

  本发明涉及一种以微小液滴形式施用活性物质的制剂，特别是具有一层或几层两亲性分子或两亲性载体物质的膜状包膜，尤其是用于将活性物质输送到或通过皮肤等天然屏障和收缩物。制剂中边缘活性物质的含量最高可达 99 摩尔%，从而达到液滴的溶解点。该制剂适用于非侵入性给药抗糖尿病药物，特别是胰岛素。本发明还涉及生产这种制剂的工艺。
• Trockenchemischer Test
  申请人：Analyticon Biotechnologies AG

  公开号：EP1544619A2

  公开（公告）日：2005-06-22

  1. Trockenchemischer Test 2. Die Erfindung betrifft ein langzeitstabilisiertes Mittel zum Nachweis von Bestandteilen in Flüssigkeiten, bestehend aus: flächenförmigemTräger, katalytisch aktiven und/oder katalytisch nichtaktiven Eiweißkörpern und monomeren oder peptidartig-verküpften Aminocarbonsäuren und Polyhydroxyverbindungen. Der Träger weist Langzeitstabilität auf.

  干化学试验 2. 本发明涉及一种用于检测液体中成分的长期稳定的方法，它包括：片状载体、具有催化活性和/或无催化活性的蛋白质体以及单体或肽状封端氨基羧酸和多羟基化合物。载体具有长期稳定性。
• Cross-linked collagen matrices and methods for their preparation
  申请人：——

  公开号：US20020019516A1

  公开（公告）日：2002-02-14

  A method for preparing cross-linked collagen and cross-linked collagen products. The method includes incubating collagen in a solution including water, at least one polar solvent and at least one sugar, to form cross-linked collagen. The solution may include a buffer having a suitable pH and ionic strength. The method may include removing excess unreacted sugar(s) and polar solvent(s) by washing the cross-linked collagen or by other methods. The method may also include dehydrating the cross-linked collagen, and may include subjecting the cross-linked collagen to critical point drying, or subjecting the collagen to drying or freeze-drying prior to cross-linking. The collagen may be prepared from atelopeptide collagen to reduce antigenicity, but may also be prepared from other suitable collagen types. The concentration and type of the polar solvent(s), the concentration and type of the reducing sugar(s), and the incubation duration may be varied to control the degree of cross-linking. The cross-linked collagen product may be in the form of a wet or dry matrix or membrane or may be suspended in a liquid in the form of an injectable preparation. The method may be applied to collagenous proteins and collagen-like peptides.

  一种制备交联胶原蛋白和交联胶原蛋白产品的方法。该方法包括在包括水、至少一种极性溶剂和至少一种糖的溶液中培养胶原蛋白，以形成交联胶原蛋白。溶液可包括具有合适 pH 值和离子强度的缓冲液。该方法可包括通过洗涤交联胶原蛋白或其他方法去除多余的未反应糖和极性溶剂。该方法还可包括使交联胶原脱水，可包括使交联胶原进行临界点干燥，或在交联之前使胶原进行干燥或冷冻干燥。为了降低抗原性，胶原蛋白可以由阿特肽胶原蛋白制备，但也可以由其他合适的胶原蛋白类型制备。可改变极性溶剂的浓度和类型、还原糖的浓度和类型以及培养时间，以控制交联程度。交联的胶原蛋白产品可以是湿的或干的基质或膜的形式，也可以是悬浮在液体中的可注射制剂的形式。该方法可用于胶原蛋白和胶原蛋白样肽。
• CLONAGE ET EXPRESSION DU GENE DE LA BETA-GLUCOSIDASE D'ASPERGILLUS ORYZAE BGII
  申请人：INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE

  公开号：EP1071791A1

  公开（公告）日：2001-01-31