<2.4-Dinitro-phenyl>-α-D-mannosid | 25694-53-5

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: <2.4-Dinitro-phenyl>-α-D-mannosid
• 英文别名: (2.4-Dinitro-phenyl)-α-D-mannosid；2,4-Dinitrophenyl alpha-D-mannopyranoside；(2R,3S,4S,5S,6R)-2-(2,4-dinitrophenoxy)-6-(hydroxymethyl)oxane-3,4,5-triol
• CAS: 25694-53-5
• 化学式: C₁₂H₁₄N₂O₁₀
• 分子量: 346.251
• InChiKey: XAJUPNGKLHFUED-GCHJQGSQSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  -0.7
• 重原子数：
  24
• 可旋转键数：
  3
• 环数：
  2.0
• sp3杂化的碳原子比例：
  0.5
• 拓扑面积：
  191
• 氢给体数：
  4
• 氢受体数：
  10

反应信息

• 作为产物：
  描述：

  甘露糖 、 2,4-二硝基氟苯 在 碳酸氢钠 作用下， 以 乙醇 为溶剂， 以25%的产率得到<2.4-Dinitro-phenyl>-α-D-mannosid
  参考文献：
  名称：

  Single Step Stereoselective Synthesis of Unprotected 2,4-Dinitrophenyl Glycosides

  摘要：

  DOI：

  10.1016/00404-0399(50)1031c-

文献信息

• Protein glycosylation modification in Pichia pastoris
  申请人：Research Corporation Technologies, Inc.

  公开号：EP2302047A1

  公开（公告）日：2011-03-30

  The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, G1cNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the contemplated vectors are also included in this invention.

  本发明提供了能够生产糖基化程度降低或修饰的蛋白质的养甲酵母（包括 Pichia，尤其是 Pichia pastoris）的基因工程菌株。本发明还提供了使用这种经基因工程改造的 Pichia 菌株生产糖基化程度降低和/或糖基化程度改变的糖蛋白的方法。本发明考虑使用包含α-1,2-甘露糖苷酶 I、葡萄糖苷酶 II、G1cNAc-转移酶 I 和甘露糖苷酶 II 的编码序列或包含 OCH1 干扰序列的载体来转化甲基营养酵母。本发明还包括提供所述载体的试剂盒。
• PROTEIN GLYCOSYLATION MODIFICATION IN METHYLOTROPHIC YEAST
  申请人：——

  公开号：US20040018588A1

  公开（公告）日：2004-01-29

  The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for &agr;-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.

  本发明提供了经基因工程改造的甲基营养酵母菌株，包括 Pichia，特别是 糊粉菌 能够生产糖基化程度降低或糖基化程度改变的蛋白质。本发明还提供了使用这种经基因工程改造的 Pichia 菌株生产糖基化程度降低和/或糖基化程度改变的糖蛋白的方法。本发明考虑使用包含&agr;-1,2-甘露糖苷酶 I、葡萄糖苷酶 II、GlcNAc-转移酶 I 和甘露糖苷酶 II 的编码序列或包含 OCH1 干扰序列的载体来转化甲基营养酵母。本发明还包括提供所述载体的试剂盒。
• PROTEIN GLYCOSYLATION MODIFICATION IN PICHIA PASTORIS
  申请人：Flanders Interuniversity Institute for Biotechnology (VIB)

  公开号：EP1516048A2

  公开（公告）日：2005-03-23
• Protein glycosylation modification in methylotrophic yeast
  申请人：Contreras Roland

  公开号：US20080009037A1

  公开（公告）日：2008-01-10

  The present invention provides genetically engineered strains of methylotrophic yeast including Pichia and especially Pichia pastoris capable of producing proteins with reduced or modified glycosylation. Methods of producing glycoproteins with reduced and/or modified glycosylation using such genetically engineered strains of Pichia are also provided. Vectors, which comprise coding sequences for α-1,2-mannosidase I, glucosidase II, GlcNAc-tranferase I and mannosidase II or comprising OCH1 disrupting sequence, for transforming methylotrophic yeasts are contemplated by the present invention. Kit for providing the comtemplated vectors are also included in this invention.
• US7252933B2
  申请人：——

  公开号：US7252933B2

  公开（公告）日：2007-08-07