3-Azidoacridin | 34947-28-9

• 分子结构分类: 有机化合物 - 有机杂环化合物 - 喹啉及其衍生物
• 英文名称: 3-Azidoacridin
• 英文别名: 3-azido-acridine；Acridine, 3-azido-；3-azidoacridine
• CAS: 34947-28-9
• 化学式: C₁₃H₈N₄
• 分子量: 220.233
• InChiKey: MDQUSQOIBBZWLQ-UHFFFAOYSA-N

计算性质

• 辛醇/水分配系数(LogP)：
  4.4
• 重原子数：
  17
• 可旋转键数：
  1
• 环数：
  3.0
• sp3杂化的碳原子比例：
  0.0
• 拓扑面积：
  27.2
• 氢给体数：
  0
• 氢受体数：
  3

文献信息

• Labelled nucleic acid probes and adducts for their preparation
  申请人：MOLECULAR DIAGNOSTICS, INC.

  公开号：EP0131830A1

  公开（公告）日：1985-01-23

  A labeled nucleic acid probe comprising (a) a nucleic acid component, (b) a nucleic acid-binding ligand photochemically linked to the nucleic acid component, and (c) a label chemically linkedto the nucleic acid-binding ligand. The label can be a specifically bindable ligand such as a hapten or biotin, an enzyme such as a ß-galactosidase or horse radish peroxidase, a fluorescent radical, a phycobiliprotein, a luminescent radical, or a radioisotope. The probe can be used in assays of nucleic acids, taking advantage of the ability of the nucleic acid component to hybridize.

  一种标记核酸探针，包括：(a) 核酸成分；(b) 与核酸成分光化学连接的核酸结合配体；(c) 与核酸结合配体化学连接的标记。标签可以是特异性可结合配体，如合蛋白或生物素；也可以是酶（如ß-半乳糖苷酶或马萝卜过氧化物酶）、荧光基、藻脂蛋白、发光基或放射性同位素。探针可用于核酸检测，利用核酸成分的杂交能力。
• Hybridization assay with immobilization of hybrids by antihybrid binding
  申请人：MILES INC.

  公开号：EP0146039A2

  公开（公告）日：1985-06-26

  A nucleic acid hybridization assay wherein the hybrid formed between the polynucleotide sequence to be detected and the probe is immobilized by binding of an immobilized or immobilizable form of an antibody reagent selective for binding such hybrid. Preferred antibody reagents are selective for DNA·RNA or RNA·RNA hybrids or for intercalated duplexes. The immobilized hybrid is detected preferably by use of a labeled form of a second such antibody reagent or a labeled form of the probe. The method dispenses with the need to immobilize either the probe or sample nucleic acids and permits hybridization to proceed between the sequence to be detected and the probe in solution, resulting in rapid rates of hybridization.

  一种核酸杂交检测方法，其中待检测的多核苷酸序列与探针之间形成的杂交体通过与选择性结合这种杂交体的固定或可固定形式的抗体试剂结合而被固定。 优选的抗体试剂对 DNA-RNA 或 RNA-RNA 杂交体或夹杂双链体具有选择性。 固定的杂交体最好通过使用标记形式的第二种抗体试剂或标记形式的探针来检测。 该方法无需固定探针或样本核酸，允许待检测序列与溶液中的探针进行杂交，从而实现快速杂交。
• Nucleic acid hybridization assay employing antibodies to intercalation complexes
  申请人：MILES INC.

  公开号：EP0146815A2

  公开（公告）日：1985-07-03

  Nucleic acid hybridization assay methods and reagent systems for detecting a particular polynucleotide sequence in a test medium. An aggregate is formed in the assay reaction mixture comprising intercalation complexes between a nucleic acid intercalator and double stranded nucleic acid associated with the hybridization product of the sequence to be detected and a nucleic acid probe sequence. Hybridization of the probe with the sequence to be detected can then be determined by addition of an antibody, or a fragment thereof, capable of binding with the intercalation complexes in the formed aggregate and measuring the antibody or fragment thereof which becomes bound to such intercalation complexes associated with hybridized probe. In one preferred embodiment, this method eliminates the need to chemically modify the probe in order to form a labeled reagent. In another embodiment, the method provides an advantageous method for labeling the probe by chemical modification.

  用于检测测试介质中特定多核苷酸序列的核酸杂交检测方法和试剂系统。在检测反应混合物中形成一个聚合体，该聚合体由核酸插入剂和双链核酸之间的插入复合物组成，双链核酸与待检测序列和核酸探针序列的杂交产物有关。然后，通过加入能与所形成的混合物中的插层复合物结合的抗体或其片段，并测量与杂交探针相关的插层复合物结合的抗体或其片段，就能确定探针与待检测序列的杂交情况。在一个优选的实施方案中，该方法无需对探针进行化学修饰以形成标记试剂。在另一个实施方案中，该方法提供了一种通过化学修饰对探针进行标记的有利方法。
• Hybridization assay employing labeled pairs of hybrid binding reagents
  申请人：MILES INC.

  公开号：EP0144914A2

  公开（公告）日：1985-06-19

  @ A nucleic acid hybridization assay wherein the hybrid formed with the probe has binding sites for two specific binding reagents, one of which comprises a first label and the other a second label, interaction of the first and second labels providing a detectable response which is measurably different when the two labeled reagents are both bound to the same hybrid compared to when the two labeled reagents are not so bound. The formation of the hybrid assay product brings the two labels within a proximate interaction distance of one another, e.g., as in the cases of sequential catalyst (enzyme) interaction and energy transfer. Since the labels provide a response which is distinguishable when they are associated with a hybridized probe, no separation step is required.

  一种核酸杂交检测方法，其中与探针形成的杂交体具有两个特异性结合试剂的结合位点，其中一个包括第一标签，另一个包括第二标签，第一标签和第二标签的相互作用提供了可检测的反应，当两个标签试剂都结合到同一个杂交体上时，与两个标签试剂没有结合到同一个杂交体上时相比，反应有可测量的差异。 杂交检测产物的形成使两个标签在彼此近似的相互作用距离内，例如，在顺序催化剂（酶）相互作用和能量转移的情况下。 由于标签在与杂交探针结合时提供了可区分的反应，因此不需要分离步骤。
• Hybridization assay employing labeled probe and anti-hybrid
  申请人：MILES INC.

  公开号：EP0144913A2

  公开（公告）日：1985-06-19

  A nucleic acid hybridization assay involving a labeled probe and formation of a hybrid having epitopes for an antibody reagent. The label provides a detectable response which is measurably different when the labeled probe is comprised in a hybrid that is bound by the antibody reagent compared to when not comprised in such a hybrid. Particularly useful antibody reagents are antibodies such as anti-DNA'RNA, anti-RNA'RNA and antibodies to intercalated duplexes which do not bind substantially to single stranded nucleic acids. Modulation of the label response can be accomplished in a variety of ways such as by steric inactivation or hindrance or by labeling the antibody reagent with a second label which interacts with the first label. The assay is particularly advantageous because no separation of hybridized and unhybridized probe is required.

  一种核酸杂交试验，涉及标记探针和具有抗体试剂表位的杂交体的形成。当标记探针与抗体试剂结合的杂交体中含有标记探针时，与不含有标记探针的杂交体中含有标记探针时相比，标记探针会产生可测量的不同反应。特别有用的抗体试剂是抗 DNA'RNA、抗 RNA'RNA 和不与单链核酸结合的夹层双链抗体。标记反应的调节可以通过多种方式实现，如立体失活或阻碍，或用与第一个标记相互作用的第二个标记对抗体试剂进行标记。由于无需分离杂交探针和未杂交探针，因此这种检测方法特别有优势。