[U-13C6]glucose | 110187-42-3

• 分子结构分类: 有机化合物 - 有机氧化合物
• 英文名称: [U-13C6]glucose
• 英文别名: 2,3,4,5,6-Pentakis(oxidanyl)hexanal；2,3,4,5,6-pentahydroxy(1,2,3,4,5,6-13C6)hexanal
• CAS: 110187-42-3
• 化学式: C₆H₁₂O₆
• 分子量: 186.092
• InChiKey: GZCGUPFRVQAUEE-IDEBNGHGSA-N

物化性质

• 熔点：
  150-152 °C(lit.)
• 溶解度：
  可溶于甲醇（轻微、加热、超声处理）、水（少量、超声处理）

计算性质

• 辛醇/水分配系数(LogP)：
  -2.9
• 重原子数：
  12
• 可旋转键数：
  5
• 环数：
  0.0
• sp3杂化的碳原子比例：
  0.83
• 拓扑面积：
  118
• 氢给体数：
  5
• 氢受体数：
  6

安全信息

• WGK Germany：
  3
• 危险性防范说明：
  P261,P280,P301+P312,P302+P352,P305+P351+P338
• 危险性描述：
  H302,H315,H319,H335

制备方法与用途

应用

13C6-葡萄糖已被用作：

• 作为从头合成脂肪酸(FA)的示踪剂，分析稳定同位素示踪剂在重组蛋白生产中的作用；
• 作为示踪介质中分析稳定同位素示踪剂的组分；
• 用于重组蛋白生产的基本培养基中；
• 用于悬浮细胞。

生物活性

D-Glucose-13C6（葡萄糖-13C6）是一种追踪与葡萄糖相关的合成分解代谢的示踪剂，它是一种低成本的替代品。其显著优势在于天然葡萄糖的第六种同位素实际上具有零自然丰度，这有助于在输注液中使用小于 1% 标记的葡萄糖进行同位素异构体分析。

文献信息

• Yeast host cells and methods for producing fatty alcohols
  申请人：D'Espaux Leopold

  公开号：US10557152B2

  公开（公告）日：2020-02-11

  The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.

  本发明提供了一种转基因酵母细胞，该细胞包含至少六种或六种以上的下列修饰：增加麝香草脂肪酸还原酶、乙酰-CoA羧化酶、脂肪酸合成酶1、脂肪酸合成酶2、对转录后和翻译后抑制不敏感的ACC1编码的瓶颈酶突变体和/或OLE1编码的去饱和酶的表达，以及减少DGA1、HFD1、ADH6和/或GDH1的表达。本发明提供了一种构建转基因酵母细胞的方法，以及一种利用转基因酵母细胞生产脂肪醇的方法。
• Yeast cells and methods for producing fatty alcohols
  申请人：The Regents of the University of California

  公开号：US11365431B2

  公开（公告）日：2022-06-21

  The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.

  本发明提供了一种转基因酵母细胞，该细胞包含至少六种或六种以上的下列修饰：增加麝香草脂肪酸还原酶、乙酰-CoA羧化酶、脂肪酸合成酶1、脂肪酸合成酶2、对转录后和翻译后抑制不敏感的ACC1编码的瓶颈酶突变体和/或OLE1编码的去饱和酶的表达，以及减少DGA1、HFD1、ADH6和/或GDH1的表达。本发明提供了一种构建转基因酵母细胞的方法，以及一种利用转基因酵母细胞生产脂肪醇的方法。
• Yeast Host Cells and Methods for Producing Fatty Alcohols
  申请人：D'Espaux Leopold

  公开号：US20190136266A1

  公开（公告）日：2019-05-09

  The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.
• Yeast Cells and Methods for Producing Fatty Alcohols
  申请人：The Regents of the University of California

  公开号：US20200231990A1

  公开（公告）日：2020-07-23

  The present invention provides for a genetically modified yeast cell comprising at least six or more of the following modifications: increased expression of Mus musculus fatty acid reductase, acetyl-CoA carboxylase, fatty acid synthase 1, fatty acid synthase 2, a mutant of the bottleneck enzyme encoded by ACC1 insensitive to post-transcriptional and post-translational repression, and/or a desaturase encoded by OLE1, and reduced expression of DGA1, HFD1, ADH6, and/or GDH1. The present invention provides a method for constructing the genetically modified yeast cell, and a method for producing a fatty alcohol from the genetically modified yeast cell.